Recombinant Human VEGF 162 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human VEGF 162 Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 1.0-5.0 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human VEGF protein
  Ala27-Arg188
• Accession #: P15692
• N-terminal Sequence: Ala27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: VEGFA
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-20 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGF 162 Protein, CF

• MVCD1
• VAS
• vascular endothelial growth factor A
• Vascular permeability factor
• Vasculotropin
• VEGF
• VEGFA
• VEGF-A
• VEGFMGC70609
• VPF
• VPFvascular endothelial growth factor

Background

The human VEGF-A gene contains eight exons plus a nontraditional ninth exon in the 3’ UTR (1). The A gene gives rise to multiple splice variants of VEGF-A, ranging from 121 amino acids (aa) to 206 aa in length. All human splice variants contain exons 1-5 and some part of exon 8. Exons 6 and 7 are split into at least two parts, some of which may be included in any one molecule (1-5). The nontraditional 9^th exon is a substitute for exon 8. Incorporation of the 9^th exon results in a non-signaling form of VEGF (5). VEGF₁₆₂ is a VEGF isoform that induces endothelial cell proliferation in vitro and angiogenesis in vivo (5). It is synthesized as a 40-45 kDa disulfide-linked homodimer whose subunits are a product of exons 1-5, 6a, 6b and 8 (5). The three aa difference between VEGF₁₆₅ and VEGF₁₆₂ is attributable to the fact that the 165 form only utilizes exon 7 while the 162 form only utilizes exon 6. Within exon 6, exon 6a contributes 24 aa while exon 6b contributes 17 aa (aa 116‑156 in the mature segment) (2). Exon 6a is not commonly found with exon 6b. In VEGF₁₆₂, exon 6b appears to act as a counterpart to the activity of exon 6a. That is, the product of exon 6a normally promotes VEGF binding to basement membrane while the product of exon 6b promotes VEGF dissociation and solubilization. In this case, VEGF₁₆₂ is notably soluble.

1. Ferrara, N. (2004) Endocrine Rev. 25:581.
2. Lange, T. et al. (2003) J. Biol. Chem. 278:17164.
3. Whittle, C. et al. (1999) Clin. Sci. 97:303.
4. Jingjing, L. et al. (1999) Invest. Ophthalmol. Vis. Sci. 40:752.
5. Woolard, J. et al. (2004) Cancer Res. 64:7822.

Publications for VEGF (2347-VE/CF)(2)

Publications using 2347-VE/CF

• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)
• Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation. J. Immunol., 2005-01-01;174(1):215-22. 2005-01-01 [PMID: 15611243] (Bioassay, Mouse)

FAQs for VEGF (2347-VE/CF). (Showing 1 - 1 of 1 FAQs).

1. Why is the molecular weight of VEGF different from the similar antibody, for some companies the the molecular weight is 40KD)?
   • I can't comment on another company's antibody because I don't have any information about their products. I can tell you that VEGF is expressed in a variety of isoforms and is subject to various post-translational modifications that influence its apparent molecular weight in an SDS-PAGE gel compared to the theoretical molecular weight.

Bioinformatics

• Gene Symbol: VEGFA
• Uniprot:
  • P15692()