Recombinant Human TWEAK R/TNFRSF12 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TWEAK R/TNFRSF12 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED 50 for this effect is 10-30 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.
• Source: Mouse myeloma cell line, NS0-derived human TWEAK R/TNFRSF12 protein

  Human TWEAK R (Glu28-Trp79) Accession # Q9NP84

  IEGRMD

  Human IgG 1 (Pro100-Lys330)

  N-terminus

  C-terminus

• Accession #: Q9NP84
• N-terminal Sequence: Glu28
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: TNFRSF12A
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 32 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 35-45 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TWEAK R/TNFRSF12 Fc Chimera Protein, CF

• CD266 antigen
• CD266
• FGF-inducible 14
• Fibroblast growth factor-inducible immediate-early response protein 14
• Fn14
• FN14tumor necrosis factor receptor superfamily member 12A
• TNFRSF12
• TNFRSF12A
• tumor necrosis factor receptor superfamily, member 12A
• TWEAK R
• TWEAKR
• tweak-receptor
• type I transmembrane protein Fn14

Background

The gene for TNF-related weak inducer of apoptosis receptor (TWEAK R) was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Human TWEAK R cDNA encodes a 129 amino acid (aa) residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain (1 - 3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain conatins one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death, cell proliferation, and angiogenesis (2 - 6). In new born mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

1. Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
2. Feng, S. et al. (2000) Am J. Pathol. 156:1253.
3. Wiley, S. et al. (2001) Immunity 15:837.
4. Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
5. Nakayama, M. et al. (2002) J. Immunol. 168:734.
6. Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

Publications for TWEAK R/TNFRSF12 (1199-TW)(2)

Publications using 1199-TW

• Jordan V. Price, David J. Haddon, Dodge Kemmer, Guillaume Delepine, Gil Mandelbaum, Justin A. Jarrell et al. Protein microarray analysis reveals BAFF-binding autoantibodies in systemic lupus erythematosus Journal of Clinical Investigation 2013-12-02 [PMID: 24270423] (Bioassay, Human)
• Dhruv H, Loftus J, Narang P, Petit J, Fameree M, Burton J, Tchegho G, Chow D, Yin H, Al-Abed Y, Berens M, Tran N, Meurice N Structural basis and targeting of the interaction between fibroblast growth factor-inducible 14 and tumor necrosis factor-like weak inducer of apoptosis. J Biol Chem, 2013-09-20;288(45):32261-76. 2013-09-20 [PMID: 24056367] (Bioassay, Human)

Bioinformatics

• Gene Symbol: TNFRSF12A
• Uniprot:
  • Q9NP84()