Recombinant Human TrkA Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human TrkA Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit NGF-induced proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.8-4 µg/mL in the presence of 10 ng/mL of rhNGF.
Source
Mouse myeloma cell line, NS0-derived human TrkA protein
Human TrkA
(Ala33-Glu407)
Accession #AAA36770
IEGRID Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala33
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
NTRK1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
67.4 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
115-125 kDa, reducing conditions
Publications
Read Publications using
175-TK in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TrkA Fc Chimera Protein, CF

  • DKFZp781I14186
  • EC 2.7.10
  • EC 2.7.10.1
  • MTChigh affinity nerve growth factor receptor
  • Neurotrophic tyrosine kinase receptor type 1
  • neurotrophic tyrosine kinase, receptor, type 1
  • NTRK1
  • NTRK-1
  • p140-TrkA
  • TRK1-transforming tyrosine kinase protein
  • TrkA
  • Trk-A
  • TRKAOncogene TRK
  • TRKTRK1
  • tyrosine kinase receptor A

Background

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, Trk B and Trk C preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily. NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when expressed independent of Trk.
  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (200) Annu. Rev. Neurosci. 24:1217.

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175-TK
Species: Hu
Applications: Bioactivity

Publications for TrkA (175-TK)(8)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bacteria, N/A.

We have publications tested in 6 applications: Bioassay, ELISA (Capture), In Vivo, In vivo, PcIP, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
ELISA (Capture)
(1)
In Vivo
(1)
In vivo
(1)
PcIP
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(2)
Mouse
(1)
Rat
(2)
Bacteria
(1)
N/A
(2)
All Species
Showing Publications 1 - 8 of 8.
Publications using 175-TK Applications Species
AJ Poole, L Frigotto, ME Smith, C Baar, G Ivanova-Be, A Jaulent, C Stace, CG Ullman, AV Hine A C-terminal cysteine residue is required for peptide-based inhibition of the NGF/TrkA interaction at nM concentrations: implications for peptide-based analgesics Sci Rep, 2019;9(1):930. 2019 [PMID: 30700786] (ELISA (Capture), Human) ELISA (Capture) Human
JM Hall, F Gomez-Pini, LM Savage Nerve Growth Factor Is Responsible for Exercise-Induced Recovery of Septohippocampal Cholinergic Structure and Function Front Neurosci, 2018;12(0):773. 2018 [PMID: 30443202] (In vivo, Rat) In vivo Rat
JI Hurtado-Za, B Ramachandr, S Ahmed, R Halder, C Bolleyer, A Awasthi, MA Stahlberg, RJ Wagener, K Anderson, RM Drenan, HA Lester, JM Miwa, JF Staiger, A Fischer, C Dean TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus Nat Commun, 2017;8(0):15878. 2017 [PMID: 28722015] (Bioassay, Mouse) Bioassay Mouse
JN Sleigh, JM Dawes, SJ West, N Wei, EL Spaulding, A Gómez-Mart, Q Zhang, RW Burgess, MZ Cader, K Talbot, XL Yang, DL Bennett, G Schiavo Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28351971] (Bioassay, N/A) Bioassay N/A
Prencipe G, Minnone G, Strippoli R, De Pasquale L, Petrini S, Caiello I, Manni L, De Benedetti F, Bracci-Laudiero L Nerve growth factor downregulates inflammatory response in human monocytes through TrkA. J Immunol, 2014;192(7):3345-54. 2014 [PMID: 24585880] (Bioassay, Human) Bioassay Human
Tsai CY, Chan JY, Hsu KS Brain-derived neurotrophic factor ameliorates brain stem cardiovascular dysregulation during experimental temporal lobe status epilepticus. PLoS ONE, 2012;7(3):e33527. 2012 [PMID: 22442695] (In Vivo, Rat) In Vivo Rat
Vaegter CB, Jansen P, Fjorback AW Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 2011;14(1):54-61. 2011 [PMID: 21102451] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Lu B, PereiraPerrin M A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 2008;76(8):3530-8. 2008 [PMID: 18541656] (PcIP, Bacteria) PcIP Bacteria

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FAQs for TrkA (175-TK). (Showing 1 - 1 of 1 FAQs).

  1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
    • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

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Blogs on TrkA.

TrkB: Bridging Ontogenesis and Oncogenesis
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. Interaction of brain-derived neurotrophic factor (BDNF) with its...  Read full blog post.

TrkB: Docking for Neurotrophins and Beyond.
Tropomyosin receptor kinase B (TrkB) is a member of the Trk receptor tyrosine kinases family consisting of TrkA, TrkB and TrkC. The sequence of these family members is highly conserved. TrK's are activated by several neurotrophins, which are small pro...  Read full blog post.

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Bioinformatics

Gene Symbol NTRK1
Uniprot