Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF

Measured by its binding ability in a functional ELISA. When Recombinant Human beta -NGF (256-GF) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag Protein (Catalog # AVI11378) binds with an ED50 of 7.00‑70.0 ng/mL.

2 μg/lane of Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag Protein (Catalog # AVI11378) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-120 kDa and 210-240 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA.

  When Recombinant Human beta -NGF (Catalog # 256-GF) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human TrkA Fc Chimera Avi-tag (Catalog # AVI11378) binds with an ED₅₀ of 7.00-70.0 ng/mL.

• Source: Chinese Hamster Ovary cell line, CHO-derived human TrkA protein
  

  Human TrkA (Ala34-Gly423) Accession # P04629.4	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: P04629.4
• N-terminal Sequence: Ala34
• Structure / Form: Disulfide-linked homodimer
  Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-120 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TrkA Fc Chimera Avi-tag Protein, CF

• DKFZp781I14186
• EC 2.7.10
• EC 2.7.10.1
• MTChigh affinity nerve growth factor receptor
• Neurotrophic tyrosine kinase receptor type 1
• neurotrophic tyrosine kinase, receptor, type 1
• NTRK1
• NTRK-1
• p140-TrkA
• TRK1-transforming tyrosine kinase protein
• TrkA
• Trk-A
• TRKAOncogene TRK
• TRKTRK1
• tyrosine kinase receptor A

Background

TrkA (Tyrosine kinase receptor A), also known as High affinity NGF receptor, is a member of the neurotrophic tyrosine kinase receptor family that has three members,  TrkA, Trk B and Trk C, which preferentially bind NGF, NT-4 and BDNF, and NT-3, respectively (1). All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct TrkA isoforms that differ by virtue of a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA is expressed mainly in non-neuronal tissues (1). NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the coexpression of TrkA with the p75 NGF receptor (NGFR), a member of the tumor necrosis factor receptor superfamily (2). NGFR binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGFR can also mediate cell death when being expressed independent of Trk (3). Our Avi-tag Biotinylated human TrkA Fc Chimeria features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Shelton D.L. et al. (1995) J. Neurosci. 15:477.
   
2. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
3. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.

FAQs for TrkA (AVI11378). (Showing 1 - 1 of 1 FAQ).

1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
   • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Bioinformatics

• Uniprot:
  • P04629.4()