Recombinant Human TRANCE/RANK L/TNFSF11 Protein


Recombinant human TRANCE / RANK L / TNFSF11 (390-TN) induces osteoclast differentiation of the RAW264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 1.5-7.5 ng/mL in the presence of 2.5 µg/mL of a more

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Applications Bioactivity

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Recombinant Human TRANCE/RANK L/TNFSF11 Protein Summary

Additional Information
A New rh TRANCE is Now Available! The new protein has no tag!
Details of Functionality
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 1.5-7.5 ng/mL in the presence of 2.5 µg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Mouse myeloma cell line, NS0-derived human TRANCE/TNFSF11/RANK L protein
Accession # AAC51762
N-terminus C-terminus
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
35 kDa, reducing conditions
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390-TN in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with BSA as a carrier protein.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TRANCE/RANK L/TNFSF11 Protein

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11


RANK Ligand (receptor activator of NF-kappa B ligand [RANKL], also called TNF-related activation-induced cytokines (TRANCE), osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. RANK Ligand was originally identified as an immediate early gene up‑regulated by T cell receptor stimulation. The human RANK Ligand cDNA encodes a type II transmembrane protein of 317 amino acids with a predicted cytoplasmic domain of 47 amino acids, a 21 amino acids transmembrane region, and an extracellular domain of 249 amino acids. The extracellular domain contains two potential N‑linked glycosylation sites. Mouse and human RANK Ligand share 85% amino acid identity. RANK Ligand is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of RANK Ligand include induction of activation of the c-jun N‑terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of RANK Ligand. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds RANK Ligand, is a naturally occurring decoy receptor that counterbalances the effects of RANK Ligand.

  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.

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Publications for TRANCE/TNFSF11/RANK L (390-TN)(37)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Equine, N/A.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Standard), Surface Plasmon Resonance (SPR.

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Cell Culture
ELISA (Standard)
Surface Plasmon Resonance (SPR
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Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using 390-TN Applications Species
D Ding, L Wang, J Yan, Y Zhou, G Feng, L Ma, Y Yang, X Pei, Q Jin Zoledronic acid generates a spatiotemporal effect to attenuate osteoarthritis by inhibiting potential Wnt5a-associated abnormal subchondral bone resorption PLoS ONE, 2022;17(7):e0271485. 2022 [PMID: 35900969] (Bioassay, Rat) Bioassay Rat
H Guo, D Ding, L Wang, J Yan, L Ma, Q Jin Metformin attenuates osteoclast-mediated abnormal subchondral bone remodeling and alleviates osteoarthritis via AMPK/NF-kappaB/ERK signaling pathway PLoS ONE, 2021;16(12):e0261127. 2021 [PMID: 34914744] (Bioassay, Mouse) Bioassay Mouse
J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research & Therapy, 2020;22(1):221. 2020 [PMID: 32958023] (Bioassay, Human) Bioassay Human
JS Brunner, L Vulliard, M Hofmann, M Kieler, A Lercher, A Vogel, M Russier, JB Brüggenthi, M Kerndl, V Saferding, B Niederreit, A Junza, A Frauenstei, C Scholtysek, Y Mikami, K Klavins, G Krönke, A Bergthaler, JJ O'Shea, T Weichhart, F Meissner, JS Smolen, P Cheng, O Yanes, J Menche, PJ Murray, O Sharif, S Blüml, G Schabbauer Environmental arginine controls multinuclear giant cell metabolism and formation Nat Commun, 2020;11(1):431. 2020 [PMID: 31969567] (Cell Culture, Human) Cell Culture Human
KA Lee, KW Kim, BM Kim, JY Won, HK Min, DW Lee, HR Kim, SH Lee Promotion of osteoclastogenesis by IL-26 in rheumatoid arthritis Arthritis Res. Ther., 2019;21(1):283. 2019 [PMID: 31831038] (Bioassay, Human) Bioassay Human
I Zoi, MV Karamouzis, E Xingi, P Sarantis, D Thomaidou, P Lembessis, S Theocharis, AG Papavassil Combining RANK/RANKL and ERBB-2 targeting as a novel strategy in ERBB-2-positive breast carcinomas Breast Cancer Res., 2019;21(1):132. 2019 [PMID: 31796128] (Bioassay, Human) Bioassay Human
K Li, S Chen, P Cai, K Chen, L Li, X Yang, J Yi, X Luo, Y Du, H Zheng MiRNA-483-5p is involved in the pathogenesis of osteoporosis by promoting osteoclast differentiation Mol. Cell. Probes, 2019;0(0):101479. 2019 [PMID: 31706013] (Cell Culture, Human) Cell Culture Human
N Ogasawara, JA Poposki, AI Klingler, BK Tan, KE Hulse, WW Stevens, AT Peters, LC Grammer, KC Welch, SS Smith, DB Conley, JR Raviv, P Soroosh, KI Takano, T Himi, RC Kern, RP Schleimer, A Kato Role of RANK-L as a potential inducer of ILC2-mediated type 2 inflammation in chronic rhinosinusitis with nasal polyps Mucosal Immunol, 2020;13(1):86-95. 2020 [PMID: 31641233] (Cell Culture, Human) Cell Culture Human
IJ Yeh, SC Chen, MC Yen, YH Wu, CH Hung, PL Kuo 6-Shogaol Suppresses 2-Amino-1-Methyl-6-Phenylimidazo [4,5-b] Pyridine (PhIP)-Induced Human 786-O Renal Cell Carcinoma Osteoclastogenic Activity and Metastatic Potential Nutrients, 2019;11(10):. 2019 [PMID: 31569368] (Bioassay, Human) Bioassay Human
FL Collins, MD Stone, J Turton, LR McCabe, ECY Wang, AS Williams Oestrogen-deficiency induces bone loss by modulating CD14+ monocyte and CD4+ T cell DR3 expression and serum TL1A levels BMC Musculoskelet Disord, 2019;20(1):326. 2019 [PMID: 31299941] (Bioassay, Human) Bioassay Human
Show All 37 Publications.

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The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Gene Symbol TNFSF11