Recombinant Human TRAIL/TNFSF10 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human TRAIL/TNFSF10 Protein, CF Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-12 ng/mL.
The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED50 for this effect is 1-4 ng/mL.
Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.
Source
Mouse myeloma cell line, NS0-derived
Thr95-Gly281, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Met
Structure / Form
Noncovalently-linked homotrimer and disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TNFSF10
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
24 kDa, reducing conditions
Publications
Read Publications using
375-TL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TRAIL/TNFSF10 Protein, CF

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).

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375-TL/CF
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Applications: BA

Publications for (24)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(23)
All Applications
Filter By Species
Human
(23)
All Species
Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using 375-TL/CF Applications Species
MJ Mitchell, J Webster, A Chung, PP Guimarães, OF Khan, R Langer Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis Nat Commun, 2017;8(0):14179. 2017 [PMID: 28317839] (Bioassay, Human) Bioassay Human
C Liang, Y Xu, G Li, T Zhao, F Xia, G Li, D Zhang, J Wu Downregulation of DcR3 sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis Onco Targets Ther, 2017;10(0):417-428. 2017 [PMID: 28176915] (Bioassay, Human) Bioassay Human
TNFSF10/TRAIL regulates human T4 effector memory lymphocyte radiosensitivity and predicts radiation-induced acute and subacute dermatitis Oncotarget, 2016;7(16):21416-27. 2016 [PMID: 26982083] (Bioassay, Human) Bioassay Human
Lizarraga F, Ceballos-Cancino G, Espinosa M, Vazquez-Santillan K, Maldonado V, Melendez-Zajgla J Tissue Inhibitor of Metalloproteinase-4 Triggers Apoptosis in Cervical Cancer Cells. PLoS ONE, 2015;10(8):e0135929. 2015 [PMID: 26291714] (Bioassay, Human) Bioassay Human
Jang, Jae Youn, Kim, Seong-Ju, Cho, Eun Kyun, Jeong, Soung Wo, Park, Eui Ju, Lee, Woong Ch, Lee, Sae Hwan, Kim, Sang Gyu, Kim, Young Se, Kim, Hong Soo, Kim, Boo Sung, Lin, Wenyu, Chung, Raymond TRAIL enhances apoptosis of human hepatocellular carcinoma cells sensitized by hepatitis C virus infection: therapeutic implications. PLoS ONE, 2014;9(6):e98171. 2014 [PMID: 24927176] (Bioassay, Human) Bioassay Human
Meng X, Koh B, Zhang J, Flatten K, Schneider P, Billadeau D, Hess A, Smith B, Karp J, Kaufmann S Poly(ADP-ribose) polymerase inhibitors sensitize cancer cells to death receptor-mediated apoptosis by enhancing death receptor expression. J Biol Chem, 2014;289(30):20543-58. 2014 [PMID: 24895135] (Bioassay, Human) Bioassay Human
Mathieu J, Zhou W, Xing Y, Sperber H, Ferreccio A, Agoston Z, Kuppusamy K, Moon R, Ruohola-Baker H Hypoxia-inducible factors have distinct and stage-specific roles during reprogramming of human cells to pluripotency. Cell Stem Cell, 2014;14(5):592-605. 2014 [PMID: 24656769] (Bioassay, Human) Bioassay Human
You B, Park W Suberoylanilide hydroxamic acid-induced HeLa cell death is closely correlated with oxidative stress and thioredoxin 1 levels. Int J Oncol, 2014;44(5):1745-55. 2014 [PMID: 24626405] (Bioassay, Human) Bioassay Human
Wehrkamp, Cody J, Gutwein, Ashley R, Natarajan, Sathish, Phillippi, Mary Ann, Mott, Justin L XIAP antagonist embelin inhibited proliferation of cholangiocarcinoma cells. PLoS ONE, 2014;9(3):e90238. 2014 [PMID: 24603802]
Kim, Seong Mu, Woo, Ji Sun, Jeong, Chang Hy, Ryu, Chung He, Jang, Jae-Deog, Jeun, Sin-Soo Potential application of temozolomide in mesenchymal stem cell-based TRAIL gene therapy against malignant glioma. Stem Cells Transl Med, 2014;3(2):172-82. 2014 [PMID: 24436439] (Bioassay, Human) Bioassay Human
Show All 24 Publications.

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Bioinformatics

Gene Symbol TNFSF10
Entrez
Uniprot