Recombinant Human TRAIL/TNFSF10 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TRAIL/TNFSF10 Protein, CF Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-12 ng/mL.
The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED50 for this effect is 1-4 ng/mL.
Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.
Source
Mouse myeloma cell line, NS0-derived human TRAIL/TNFSF10 protein
Thr95-Gly281, with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
Met
Structure / Form
Noncovalently-linked homotrimer and disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TNFSF10
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
21 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
24 kDa, reducing conditions
Publications
Read Publications using
375-TL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TRAIL/TNFSF10 Protein, CF

  • Apo-2 ligand
  • APO2L
  • Apo-2Ltumor necrosis factor (ligand) family, member 10
  • APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
  • CD253 antigen
  • CD253
  • Protein TRAIL
  • TL2
  • TNF-related apoptosis-inducing ligand
  • TNFSF10
  • TRAIL
  • TRAILTNF-related apoptosis inducing ligand TRAIL
  • tumor necrosis factor (ligand) superfamily, member 10
  • tumor necrosis factor ligand superfamily member 10

Background

TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).

  1. Golstein, P. (1997) Current Biology 7:R750.
  2. Wiley, S.R. et al. (1995) Immunity 3:673.
  3. Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
  4. Emery, J. et al. (1998) J. Biol. Chem. 273:14363.

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375-TL/CF
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Applications: Bioactivity

Publications for TRAIL/TNFSF10 (375-TL/CF)(30)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(29)
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Filter By Species
Human
(29)
All Species
Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 375-TL/CF Applications Species
DY Liang, W Huang, Q Chang, YQ Hou ShDcR3 sensitizes TRAIL-resistant HCC cells by inducing caspase-dependent apoptosis while suppressing NF-?B dependent cFLIPL expression PLoS ONE, 2018;13(2):e0191545. 2018 [PMID: 29444104] (Bioassay, Human) Bioassay Human
SH Hong, C Min, Y Jun, DJ Lee, SH Kim, JH Park, JH Cheong, YJ Park, SY Kim, S Lee, SW Kang Silencing of peroxiredoxin II by promoter methylation is necessary for the survival and migration of gastric cancer cells Exp. Mol. Med., 2018;50(2):e443. 2018 [PMID: 29422545] (Bioassay, Human) Bioassay Human
SM Woo, KJ Min, SU Seo, S Kim, JW Park, DK Song, HS Lee, SH Kim, TK Kwon Up-regulation of 5-lipoxygenase by inhibition of cathepsin G enhances TRAIL-induced apoptosis through down-regulation of survivin Oncotarget, 2017;8(63):106672-106684. 2017 [PMID: 29290980] (Bioassay, Human) Bioassay Human
P Zhang, H Kawakami, W Liu, X Zeng, K Strebhardt, K Tao, S Huang, FA Sinicrope Targeting CDK1 and MEK/ERK Overcomes Apoptotic Resistance in BRAF Mutated human Colorectal Cancer Mol. Cancer Res., 2017;0(0):. 2017 [PMID: 29233910] (Bioassay, Human) Bioassay Human
J Yang, Y Wu, X Wang, L Xu, X Zhao, Y Yang Chemoresistance is associated with overexpression of HAX-1, inhibition of which resensitizes drug-resistant breast cancer cells to chemotherapy Tumour Biol, 2017;39(3):1010428317692. 2017 [PMID: 28347249] (Bioassay, Human) Bioassay Human
MJ Mitchell, J Webster, A Chung, PP Guimarães, OF Khan, R Langer Polymeric mechanical amplifiers of immune cytokine-mediated apoptosis Nat Commun, 2017;8(0):14179. 2017 [PMID: 28317839] (Bioassay, Human) Bioassay Human
SH Lee, SK Hyun, HB Kim, CD Kang, SH Kim Potential Role of CD133 Expression in the Susceptibility of Human Liver Cancer Stem-Like Cells to TRAIL Oncol. Res., 2016;24(6):495-509. 2016 [PMID: 28281970] (Bioassay, Human) Bioassay Human
C Liang, Y Xu, G Li, T Zhao, F Xia, G Li, D Zhang, J Wu Downregulation of DcR3 sensitizes hepatocellular carcinoma cells to TRAIL-induced apoptosis Onco Targets Ther, 2017;10(0):417-428. 2017 [PMID: 28176915] (Bioassay, Human) Bioassay Human
TNFSF10/TRAIL regulates human T4 effector memory lymphocyte radiosensitivity and predicts radiation-induced acute and subacute dermatitis Oncotarget, 2016;7(16):21416-27. 2016 [PMID: 26982083] (Bioassay, Human) Bioassay Human
Lizarraga F, Ceballos-Cancino G, Espinosa M, Vazquez-Santillan K, Maldonado V, Melendez-Zajgla J Tissue Inhibitor of Metalloproteinase-4 Triggers Apoptosis in Cervical Cancer Cells. PLoS ONE, 2015;10(8):e0135929. 2015 [PMID: 26291714] (Bioassay, Human) Bioassay Human
Show All 30 Publications.

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Bioinformatics

Gene Symbol TNFSF10
Entrez
Uniprot