Recombinant Human TRAIL/TNFSF10 Protein, CF Summary
Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 4-12 ng/mL. The apoptotic effect of soluble trimeric rhTRAIL can be enhanced by oligimerization of the soluble rhTRAIL through the use of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). The ED50 for this effect is 1-4 ng/mL. Note: This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the variants.
Mouse myeloma cell line, NS0-derived human TRAIL/TNFSF10 protein Thr95-Gly281, with an N-terminal Met and 6-His tag
Noncovalently-linked homotrimer and disulfide-linked homodimer
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<1.0 EU per 1 μg of the protein by the LAL method.
21 kDa (monomer). Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
tumor necrosis factor (ligand) superfamily, member 10
tumor necrosis factor ligand superfamily member 10
TRAIL (TNF-related apoptosis-inducing ligand), also known as APO-2 ligand, is a type II transmembrane protein with a carboxy-terminal extracellular domain which exhibits homology to other TNF family members. In the new TNF superfamily nomenclature, TRAIL is referred to as TNFSF10. Human TRAIL cDNA encodes a 281 amino acid (aa) residue protein with an amino-terminal intracellular domain of 17 residues and a predicted internal hydrophobic domain between residues 18 and 38. The extracellular carboxy-terminal domain contains a potential N-linked glycosylation site at amino acid residue 109. Among TNF family members, TRAIL is most homologous to FAS ligand, sharing 28% amino acid sequence identity in their extracellular domains. Mouse TRAIL has also been cloned. The human TRAIL shares 65% amino acid sequence identity with mouse TRAIL and is active on mouse cells. Both membrane bound and soluble TRAIL have been shown to induce rapid apoptosis of many transformed cell lines. Like most TNF family members, the bioactive TRAIL exists as a homotrimer. TRAIL transcripts have been shown to be constitutively expressed in a variety of human tissues. A family of TRAIL receptors, including two receptors that tranduce the apoptotic signals and two TRAIL decoy receptors that function to antagonize TRAIL-induced apoptosis, have been identified (1 - 3). Osteoprotegerin has been identified as a fifth TRAIL receptor (4).
Golstein, P. (1997) Current Biology 7:R750.
Wiley, S.R. et al. (1995) Immunity 3:673.
Pitti, R.M. et al. (1996) J. Biol. Chem. 271:12687.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human TRAIL/TNFSF10 Protein, CF and receive a gift card or discount.