Recombinant Human TGF-beta 2 Protein, CF


Recombinant Human TGF-beta 2 (Catalog # 302-B2/CF) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced cell proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.025-0.25 ng/mL.
1 μg/lane of Recombinant Human TGF-beta 2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human TGF-beta 2 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.025-0.25 ng/mL.
Mouse myeloma cell line, NS0-derived human TGF-beta 2 protein
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
12.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
12 kDa, reducing conditions
24 kDa, non-reducing condtions
Read Publications using
302-B2/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute 2 µg vial at 5 µg/mL in sterile 4 mM HCl. Reconstitute 10 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 2 Protein, CF

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2


TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA, ICC, WB
Species: Mu
Applications: ELISA
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, IHC, Neut, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: IHC
Species: Hu
Applications: BA
Species: Hu
Applications: Bioactivity

Publications for TGF-beta 2 (302-B2/CF)(82)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Canine, Chicken, Cynomolgus Monkey.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.

Filter By Application
Cell Culture
ELISA (Standard)
In Vivo
Surface Plasmon Resonance
Western Blot
All Applications
Filter By Species
Cynomolgus Monkey
All Species
Showing Publications 1 - 10 of 82. Show All 82 Publications.
Publications using 302-B2/CF Applications Species
Ma, HY;Li, Q;Wong, WR;N'Diaye, EN;Caplazi, P;Bender, H;Huang, Z;Arlantico, A;Jeet, S;Wong, A;Emson, C;Brightbill, H;Tam, L;Newman, R;Roose-Girma, M;Sandoval, W;Ding, N; LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lung Science advances 2023-05-26 [PMID: 37235663] (Bioassay, Human) Bioassay Human
H Han, Y Yang, Z Han, L Wang, L Dong, H Qi, B Liu, J Tian, B Vanhaesebr, A Kazlauskas, G Zhang, S Zhang, H Lei NFkappaB-Mediated Expression of Phosphoinositide 3-Kinase delta Is Critical for Mesenchymal Transition in Retinal Pigment Epithelial Cells Cells, 2023-01-04;12(2):. 2023-01-04 [PMID: 36672142] (Bioassay, Human) Bioassay Human
M Morita, A Yoneda, N Tokunoh, T Masaki, K Shirakura, M Kinoshita, R Hashimoto, N Shigesada, J Takahashi, M Tachibana, S Tanaka, M Obana, N Hino, M Ikawa, K Tsujikawa, C Ono, Y Matsuura, H Kidoya, N Takakura, Y Kubota, T Doi, K Takayama, Y Yoshioka, Y Fujio, Y Okada Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models Proceedings of the National Academy of Sciences of the United States of America, 2023-01-12;120(3):e2213317120. 2023-01-12 [PMID: 36634143] (Bioassay, Human) Bioassay Human
B Kramer, C Corallo, A van den He, J Crawford, T Olivier, E Elstak, N Giordano, P Vulto, HL Lanz, RAJ Janssen, MA Tessari High-throughput 3D microvessel-on-a-chip model to study defective angiogenesis in systemic sclerosis Scientific Reports, 2022-10-08;12(1):16930. 2022-10-08 [PMID: 36209279] (Bioassay, Human) Bioassay Human
K Santerre, S Cortez Ghi, S Proulx TGF-beta-Mediated Modulation of Cell-Cell Interactions in Postconfluent Maturing Corneal Endothelial Cells Investigative Ophthalmology & Visual Science, 2022-10-03;63(11):3. 2022-10-03 [PMID: 36194422] (Bioassay, Human) Bioassay Human
J Knox, G Bou-Ghario, KJ Hamill, CE Willoughby MiR-18a-5p Targets Connective Tissue Growth Factor Expression and Inhibits Transforming Growth Factor beta2-Induced Trabecular Meshwork Cell Contractility Genes, 2022-08-22;13(8):. 2022-08-22 [PMID: 36011411] (Bioassay, Human) Bioassay Human
B Callaghan, K Lester, B Lane, X Fan, K Goljanek-W, DA Simpson, C Sheridan, CE Willoughby Genome-wide transcriptome profiling of human trabecular meshwork cells treated with TGF-beta2 Oncogene, 2022-06-10;12(1):9564. 2022-06-10 [PMID: 35689009] (Bioassay, Human) Bioassay Human
A Utsunomiya, T Chino, H Kasamatsu, T Hasegawa, N Utsunomiya, VH Luong, T Matsushita, Y Sasaki, D Ogura, SI Niwa, N Oyama, M Hasegawa The compound LG283 inhibits bleomycin-induced skin fibrosis via antagonizing TGF-beta signaling Arthritis Research & Therapy, 2022-04-29;24(1):94. 2022-04-29 [PMID: 35488265] (Bioassay, Human) Bioassay Human
K Sobierajsk, ME Wawro, J Niewiarows Oxidative Stress Enhances the TGF-beta2-RhoA-MRTF-A/B Axis in Cells Entering Endothelial-Mesenchymal Transition International Journal of Molecular Sciences, 2022-02-13;23(4):. 2022-02-13 [PMID: 35216178] (Bioassay, Human) Bioassay Human
RB Kasetti, P Maddineni, B Kodati, B Nagarajan, S Yacoub Astragaloside IV Attenuates Ocular Hypertension in a Mouse Model of TGFbeta2 Induced Primary Open Angle Glaucoma International Journal of Molecular Sciences, 2021-11-19;22(22):. 2021-11-19 [PMID: 34830390] (Bioassay, Human) Bioassay Human
Show All 82 Publications.

Reviews for TGF-beta 2 (302-B2/CF) (0)

There are no reviews for TGF-beta 2 (302-B2/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for TGF-beta 2 (302-B2/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TGF-beta 2 Products

Bioinformatics Tool for TGF-beta 2 (302-B2/CF)

Discover related pathways, diseases and genes to TGF-beta 2 (302-B2/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TGF-beta 2 (302-B2/CF)

Discover more about diseases related to TGF-beta 2 (302-B2/CF).

Pathways for TGF-beta 2 (302-B2/CF)

View related products by pathway.

PTMs for TGF-beta 2 (302-B2/CF)

Learn more about PTMs related to TGF-beta 2 (302-B2/CF).

Blogs on TGF-beta 2

There are no specific blogs for TGF-beta 2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human TGF-beta 2 Protein, CF and receive a gift card or discount.


Gene Symbol TGFB2