Recombinant Human TGF-beta 2 Protein, CF

Images

 
1 μg/lane of Recombinant Human TGF-beta 2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 12 kDa and 24 kDa, respectively.
Recombinant Human TGF-beta 2 (Catalog # 302-B2/CF) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced cell proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.025-0.25 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Catalog# & Formulation Size Price

Recombinant Human TGF-beta 2 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 0.025-0.25 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human TGF-beta 2 protein
Ala303-Ser414
Accession #
N-terminal Sequence
Ala303
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
12.7 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
24 kDa, non-reducing condtions
Publications
Read Publications using
302-B2/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute 2 µg vial at 5 µg/mL in sterile 4 mM HCl. Reconstitute 10 µg or larger vials at 100 µg/mL in sterile 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-beta 2 Protein, CF

  • BSC-1 cell growth inhibitor
  • cetermin
  • Glioblastoma-derived T-cell suppressor factor
  • G-TSF
  • MGC116892
  • polyergin
  • TGFB2
  • TGFbeta 2
  • TGF-beta 2
  • TGF-beta2
  • TGF-beta-2
  • transforming growth factor beta-2
  • transforming growth factor, beta 2

Background

TGF-beta 2 (transforming growth factor beta 2) is one of three closely related mammalian members of the large TGF-beta  superfamily that share a characteristic cysteine knot structure (1-7). TGF-beta 1, -2 and -3 are highly pleiotropic cytokines proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition (1-4). Each TGF-beta isoform has some non-redundant functions; for TGF-beta 2, mice with targeted deletion show defects in development of cardiac, lung, craniofacial, limb, eye, ear and urogenital systems (2). Human TGF-beta 2 cDNA encodes a 414 amino acid (aa) precursor that contains a 19 aa signal peptide and a 395 aa proprotein (8). A furin-like convertase processes the proprotein to generate an N-terminal 232 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 2 (8, 9). Disulfide-linked homodimers of LAP and TGF-beta 2 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (8-10). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature human TGF-beta 2 shows 100% aa identity with porcine, canine, equine and bovine TGF-beta 2, and 97% aa identity with mouse and rat TGF-beta 2. It demonstrates cross-species activity (1). TGF-beta 2 signaling begins with binding to a complex of the accessory receptor betaglycan (also known as TGF-beta  RIII) and a type II ser/thr kinase receptor termed TGF-beta  RII. This receptor then phosphorylates and activates another ser/thr kinase receptor, TGF-beta  RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK-1. The whole complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Use of other signaling pathways that are Smad-independent allows for disparate actions observed in response to TGF-beta in different contexts (11).

  1. Sporn, M.B. (2006) Cytokine Growth Factor Rev. 17:3.
  2. Dunker, N. and K. Krieglstein, 2000, Eur. J. Biochem. 267:6982.
  3. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  4. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  5. Lin, J.S. et al. (2006) Reproduction 132:179.
  6. Hinck, A.P. et al. (1996) Biochemistry 35:8517.
  7. Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
  8. deMartin, R. et al. (1987) EMBO J. 6:3673.
  9. Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
  10. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  11. de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
  12. Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.

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Publications for TGF-beta 2 (302-B2/CF)(66)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, Cynomolgus Monkey, N/A.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance, Western Blot.


Filter By Application
Bioassay
(56)
Cell Culture
(4)
ELISA (Standard)
(2)
In Vivo
(2)
Surface Plasmon Resonance
(1)
Western Blot
(1)
All Applications
Filter By Species
Human
(46)
Mouse
(8)
Rat
(3)
Canine
(3)
Chicken
(1)
Cynomolgus Monkey
(1)
N/A
(3)
All Species
Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using 302-B2/CF Applications Species
E Takahashi, J Saruwatari, T Fujimoto, Y Tanoue, T Fukuda, T Inoue The effects of exosomes derived from trabecular meshwork cells on Schlemm&#039;s canal endothelial cells Scientific Reports, 2021;11(1):21942. 2021 [PMID: 34754027] (Bioassay, Cynomolgus Monkey) Bioassay Cynomolgus Monkey
CC Hsiao, YC Chang, YT Hsiao, PH Chen, MC Hsieh, WC Wu, YH Kao Triamcinolone acetonide modulates TGF?beta2?induced angiogenic and tissue?remodeling effects in cultured human retinal pigment epithelial cells Molecular Medicine Reports, 2021;24(5):. 2021 [PMID: 34523693] (Bioassay, Human) Bioassay Human
T Tabib, M Huang, N Morse, A Papazoglou, R Behera, M Jia, M Bulik, DE Monier, PV Benos, W Chen, R Domsic, R Lafyatis Myofibroblast transcriptome indicates SFRP2hi fibroblast progenitors in systemic sclerosis skin Nature Communications, 2021;12(1):4384. 2021 [PMID: 34282151] (Bioassay, Human) Bioassay Human
WT Shelton, SM Thomas, HR Alexander, CE Thomes, DE Conway, AD Dubash Desmoglein-2 harnesses a PDZ-GEF2/Rap1 signaling axis to control cell spreading and focal adhesions independent of cell-cell adhesion Scientific Reports, 2021;11(1):13295. 2021 [PMID: 34168237] (Cell Culture, Human) Cell Culture Human
T Matsumura, T Fujimoto, A Futakuchi, Y Takihara, F Watanabe-K, E Takahashi, M Inoue-Moch, H Tanihara, T Inoue TGF-&amp;beta-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness PLoS ONE, 2020;15(11):e0242626. 2020 [PMID: 33206726] (Cell Culture, Human) Cell Culture Human
F Yemanyi, J Vranka, VK Raghunatha Glucocorticoid-induced cell-derived matrix modulates transforming growth factor &amp;beta2 signaling in human trabecular meshwork cells Scientific Reports, 2020;10(1):15641. 2020 [PMID: 32973273] (Bioassay, Human) Bioassay Human
Y Fan, J Wei, L Guo, S Zhao, C Xu, H Sun, T Guo Osthole Reduces Mouse IOP Associated With Ameliorating Extracellular Matrix Expression of Trabecular Meshwork Cell Invest. Ophthalmol. Vis. Sci., 2020;61(10):38. 2020 [PMID: 32821914] (Cell Culture, Human) Cell Culture Human
SK Meurer, O Tezcan, T Lammers, R Weiskirche Differential regulation of Lipocalin 2 (LCN2) in doxorubicin-resistant 4T1 triple negative breast cancer cells Cell. Signal., 2020;74(0):109731. 2020 [PMID: 32758668] (Bioassay, Human) Bioassay Human
DC Briggs, AWW Langford-S, HL Birchenoug, TA Jowitt, CM Kielty, JJ Enghild, C Baldock, CM Milner, AJ Day Inter-&amp;alpha-inhibitor heavy chain-1 has an integrin-like 3D structure mediating immune regulatory activities and matrix stabilization during ovulation J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32144206] (Surface Plasmon Resonance) Surface Plasmon Resonance
H Yonemura, A Futakuchi, M Inoue-Moch, T Fujimoto, E Takahashi, H Tanihara, T Inoue DNA methyltransferase inhibitor suppresses fibrogenetic changes in human conjunctival fibroblasts Mol. Vis., 2019;25(0):382-390. 2019 [PMID: 31523116] (Bioassay, Human) Bioassay Human
Show All 66 Publications.

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Bioinformatics

Gene Symbol TGFB2
Uniprot