Recombinant Human ST2/IL-33R His-tag Protein, CF

When Recombinant Human ST2/IL-33R His-tag Protein (Catalog # 11272-ST) is immobilized at 1 μg/mL (100 µL/well), Recombinant Human IL-33 (3625-IL) binds with an ED50 of 10.0-80.0 ng/mL.

2 μg/lane of Recombinant Human ST2/IL-33R His-tag Protein (Catalog # 11272-ST) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-62 kDa.

• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human ST2/IL-33R His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human ST2/IL-33R His-tag (Catalog # 11272-ST) is immobilized at 1 μg/mL (100 µL/well), Recombinant Human IL-33 (Catalog # 3625-IL) binds with an ED₅₀ of 10.0-80.0 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human ST2/IL-33R protein
  Lys19-Ser328, with a C-terminal 6-His tag
• Accession #: Q01638.4
• N-terminal Sequence: Lys19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-62 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ST2/IL-33R His-tag Protein, CF

• DER4
• DER4ST2growth stimulation-expressed
• Fit-1
• IL-1 R4
• IL1R4
• IL-1R4
• IL-1RL1
• IL-33 R
• IL33R
• IL-33R
• interleukin 1 receptor-like 1
• interleukin 1 receptor-related protein
• interleukin-1 receptor-like 1
• Ly84
• MGC32623
• Protein ST2
• ST2
• ST2L
• ST2V
• T1
• T1homolog of mouse growth stimulation-expressed

Background

Serum stimulation-2 (ST2), also known as Interleukin receptor like-1 (IL1RL1) and T1, is a member of the Interleukin-1 receptor superfamily family glycoprotein that contributes to Th2 immune responses (1, 2). Human ST2 consists of an extracellular domain (ECD) with three Ig-like domains, a transmembrane segment, and a cytoplasmic domain with an intracellular Toll/interleukin-1 receptor (TIR) domain (3, 4). Within the ECD, human ST2 shares 68% and 64% amino acid sequence identity with mouse and rat ST2, respectively. Alternate splicing of human ST2 generates a soluble isoform that lacks the transmembrane and cytoplasmic regions as well as an isoform that additionally lacks the third Ig‑like domain (4). ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (5-8). It binds IL33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (5, 9). IL-33 binding induces the association of ST2 with IL1R AcP, a shared signaling subunit that also associates with IL1RI and IL1R rp2 (1, 10, 11). In macrophages, ST2 interferes with signaling from IL1RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (7). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (5, 12-14). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (6, 8, 15). Soluble ST2 functions as a decoy receptor that blocks IL33 signaling by full-length ST2 (10, 13‑15). 

1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
3. Tominaga, S. et al. (1992) Biochim. Biophys. Acta. 1171:215.
4. Li, H. et al. (2000) Genomics 67:284.
5. Schmitz, J. et al. (2005) Immunity 23:479.
6. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
7. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
8. Weinberg, E.O. et al. (2002) Circulation 106:2961.
9. Sanada S. et al. (2007) J. Clin. Invest. 117:1538.
10. Palmer, G. et al. (2008) Cytokine 42:358.
11. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
12. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
13. Verri, Jr. W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
14. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
15. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.

Bioinformatics

• Uniprot:
  • Q01638.4()