Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

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1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029 is a matrix-associated artifact of the ...read more
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Summary

Additional Information
A New rh Shh is Available! C-term cholesterol, N-term fatty acid-modified; 250-fold activity!
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 µg/mL.
Source
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
SHH
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22 kDa, reducing conditions
Publications
Read Publications using
1845-SH in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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1845-SH
Species: Hu
Applications: Bioactivity

Publications for Sonic Hedgehog/Shh (1845-SH)(26)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Xenopus.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(26)
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Human
(20)
Mouse
(3)
Rat
(1)
Bovine
(1)
Xenopus
(1)
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Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using 1845-SH Applications Species
KKB Tan, WWM Lim, C Chai, M Kukumberg, KL Lim, ELK Goh, EKF Yim Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells Sci Rep, 2018;8(1):9567. 2018 [PMID: 29934644] (Bioassay, Human) Bioassay Human
C Xu, C Zou, M Hussain, W Shi, Y Shao, Z Jiang, X Wu, M Lu, J Wu, Q Xie, Y Ke, F Long, L Tang, X Wu High expression of Sonic hedgehog in allergic airway epithelia contributes to goblet cell metaplasia Mucosal Immunol, 2018;0(0):. 2018 [PMID: 29867080] (Bioassay, Human) Bioassay Human
X Hu, JW Lee, X Zheng, J Zhang, X Lin, Y Song, B Wang, X Hu, HH Chang, Y Chen, CP Lin, Y Zhang Efficient induction of functional ameloblasts from human keratinocyte stem cells Stem Cell Res Ther, 2018;9(1):126. 2018 [PMID: 29720250] (Bioassay, Human) Bioassay Human
JY Kim, JS Lee, HS Hwang, DR Lee, CY Park, SJ Jung, YR You, DS Kim, DW Kim Wnt signal activation induces midbrain specification through direct binding of the beta-catenin/TCF4 complex to the EN1 promoter in human pluripotent stem cells Exp. Mol. Med., 2018;50(4):24. 2018 [PMID: 29650976] (Bioassay, Human) Bioassay Human
C Chen, MB Breslin, MS Lan Sonic hedgehog signaling pathway promotes INSM1 transcription factor in neuroendocrine lung cancer Cell. Signal., 2018;46(0):83-91. 2018 [PMID: 29501727] (Bioassay, Human) Bioassay Human
X Ye, W Hong, B Hao, G Peng, L Huang, Z Zhao, Y Zhou, M Zheng, C Li, C Liang, E Yi, J Pu, B Li, P Ran PM2.5 promotes human bronchial smooth muscle cell migration via the sonic hedgehog signaling pathway Respir. Res., 2018;19(1):37. 2018 [PMID: 29499705] (Bioassay, Human) Bioassay Human
G Shall, M Menosky, S Decker, P Nethala, R Welchko, X Leveque, M Lu, M Sandstrom, U Hochgeschw, J Rossignol, G Dunbar Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2018;19(3):. 2018 [PMID: 29498713] (Bioassay, Rat) Bioassay Rat
DM Fink, MR Sun, GW Heyne, JL Everson, HM Chung, S Park, MD Sheets, RJ Lipinski Coordinated d-cyclin/Foxd1 activation drives mitogenic activity of Sonic Hedgehog signaling pathway Cell. Signal., 2017;0(0):. 2017 [PMID: 29284139] (Bioassay, Mouse) Bioassay Mouse
SY Xie, G Li, C Han, YY Yu, N Li RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer Onco Targets Ther, 2017;10(0):5605-5619. 2017 [PMID: 29200875] (Bioassay, Human) Bioassay Human
S Kasiri, C Shao, B Chen, AN Wilson, P Yenerall, BC Timmons, L Girard, H Tian, C Behrens, II Wistuba, AF Gazdar, J Kim GLI1 blockade potentiates the antitumor activity of PI3K antagonists in lung squamous cell carcinoma Cancer Res., 2017;0(0):. 2017 [PMID: 28652248] (Bioassay, Human) Bioassay Human
Show All 26 Publications.

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Bioinformatics

Gene Symbol SHH
Uniprot