Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus


1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029 is a matrix-associated artifact of the more
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog # 1845-SH) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus Summary

Additional Information
A New rh Shh is Available! C-term cholesterol, N-term fatty acid-modified; 14-fold better activity!
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 µg/mL.
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
22 kDa, reducing conditions
Read Publications using
1845-SH in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT


Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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Publications for Sonic Hedgehog/Shh (1845-SH)(35)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Xenopus.

We have publications tested in 2 applications: Bioassay, Cell Culture.

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Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using 1845-SH Applications Species
WW Feng, O Wilkins, S Bang, M Ung, J Li, J An, C Del Genio, K Canfield, J DiRenzo, W Wells, A Gaur, RB Robey, JY Guo, RL Powles, C Sotiriou, L Pusztai, M Febbraio, C Cheng, WB Kinlaw, M Kurokawa CD36-Mediated Metabolic Rewiring of Breast Cancer Cells Promotes Resistance to HER2-Targeted Therapies Cell Rep, 2019;29(11):3405-3420.e5. 2019 [PMID: 31825825] (Bioassay, Mouse) Bioassay Mouse
J Ferent, S Constable, ED Gigante, PT Yam, LE Mariani, E Legué, KF Liem, T Caspary, F Charron The Ciliary Protein Arl13b Functions Outside of the Primary Cilium in Shh-Mediated Axon Guidance Cell Rep, 2019;29(11):3356-3366.e3. 2019 [PMID: 31825820] (Bioassay, Mouse) Bioassay Mouse
CL White, WSN Jayasekara, D Picard, J Chen, DN Watkins, JE Cain, M Remke, DJ Gough A Sexually Dimorphic Role for STAT3 in Sonic Hedgehog Medulloblastoma Cancers (Basel), 2019;11(11):. 2019 [PMID: 31683879] (Cell Culture, Mouse) Cell Culture Mouse
JP Zanin, JL Verpeut, Y Li, MW Shiflett, SS Wang, V Santhakuma, WJ Friedman The p75NTR influences cerebellar circuit development and adult behavior via regulation of cell cycle duration of granule cell progenitors J. Neurosci., 2019;0(0):. 2019 [PMID: 31582529] (Bioassay, Rat) Bioassay Rat
L Ma, Y Wang, Y Hui, Y Du, Z Chen, H Feng, S Zhang, N Li, J Song, Y Fang, X Xu, L Shi, B Zhang, J Cheng, S Zhou, L Liu, X Zhang WNT/NOTCH Pathway Is Essential for the Maintenance and Expansion of Human MGE Progenitors Stem Cell Reports, 2019;0(0):. 2019 [PMID: 31056478] (Bioassay, Human) Bioassay Human
SK Riaz, Y Ke, F Wang, MA Kayani, MFA Malik Influence of SHH/GLI1 axis on EMT mediated migration and invasion of breast cancer cells Sci Rep, 2019;9(1):6620. 2019 [PMID: 31036836] (Bioassay, Human) Bioassay Human
M Elkouris, G Kouroupi, A Vourvoukel, N Papagianna, V Kaltezioti, R Matsas, L Stefanis, M Xilouri, PK Politis Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson&#039;s Disease Patients Front Cell Neurosci, 2019;13(0):58. 2019 [PMID: 30853899] (Bioassay, Human) Bioassay Human
J Ooi, SR Langley, X Xu, KH Utami, B Sim, Y Huang, NP Harmston, YL Tay, A Ziaei, R Zeng, D Low, F Aminkeng, RM Sobota, F Ginhoux, E Petretto, MA Pouladi Unbiased Profiling of Isogenic Huntington Disease hPSC-Derived CNS and Peripheral Cells Reveals Strong Cell-Type Specificity of CAG Length Effects Cell Rep, 2019;26(9):2494-2508.e7. 2019 [PMID: 30811996]
C Lang, KR Campbell, BJ Ryan, P Carling, M Attar, J Vowles, OV Perestenko, R Bowden, F Baig, M Kasten, MT Hu, SA Cowley, C Webber, R Wade-Marti Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes Cell Stem Cell, 2018;0(0):. 2018 [PMID: 30503143]
KKB Tan, WWM Lim, C Chai, M Kukumberg, KL Lim, ELK Goh, EKF Yim Sequential Application of Discrete Topographical Patterns Enhances Derivation of Functional Mesencephalic Dopaminergic Neurons from Human Induced Pluripotent Stem Cells Sci Rep, 2018;8(1):9567. 2018 [PMID: 29934644] (Bioassay, Human) Bioassay Human
Show All 35 Publications.

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Gene Symbol SHH