Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF


Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog #1845-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 more
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029 is a matrix-associated artifact of the more

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Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF Summary

Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 µg/mL.
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
22 kDa, reducing conditions
Read Publications using
1845-SH/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT


Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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Publications for Sonic Hedgehog/Shh (1845-SH/CF)(58)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Porcine, Xenopus.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Pull-Down Assay.

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Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using 1845-SH/CF Applications Species
Ö Karabiyik, GD Nozhatzade, A Tuncer, G Torun Köse, E Hacihasano, F Sahin, E Aysan Production of parathyroid-like cells from thyroid stem cells in co-culture environment Medicine, 2022-12-02;101(48):e32009. 2022-12-02 [PMID: 36482540] (Bioassay, Human) Bioassay Human
X Li, PJ Gordon, JA Gaynes, AW Fuller, R Ringuette, CP Santiago, V Wallace, S Blackshaw, P Li, EM Levine Lhx2 is a progenitor-intrinsic modulator of Sonic Hedgehog signaling during early retinal neurogenesis Elife, 2022-12-02;11(0):. 2022-12-02 [PMID: 36459481] (Cell Culture, Mouse) Cell Culture Mouse
DS Lee, YJ Song, HR Gug, JH Lee, HS Bae, JC Park Nuclear Factor I-C Regulates Stemness Genes and Proliferation of Stem Cells in Various Mineralized Tissue through Epithelial-Mesenchymal Interactions in Dental Epithelial Stem Cells Stem Cells International, 2022-09-27;2022(0):1092184. 2022-09-27 [PMID: 36213683] (Cell Culture, Mouse) Cell Culture Mouse
JS Fleck, SMJ Jansen, D Wollny, F Zenk, M Seimiya, A Jain, R Okamoto, M Santel, Z He, JG Camp, B Treutlein Inferring and perturbing cell fate regulomes in human brain organoids Nature, 2022-10-05;0(0):. 2022-10-05 [PMID: 36198796] (Bioassay, Human) Bioassay Human
MA Hossain, M Hasegawa-O, Y Manome, M Igarashi, C Wu, K Suzuki, J Igarashi, T Iwamoto, HJ Okano, Y Eto Generation and characterization of motor neuron progenitors and motor neurons using metachromatic leukodystrophy-induced pluripotent stem cells Oncogene, 2022-02-21;31(0):100852. 2022-02-21 [PMID: 35782608] (Bioassay, Human) Bioassay Human
JSY Tan, B Lee, J Lim, DR Ma, JX Goh, SY Goh, MY Gulam, SM Koh, WW Lee, L Feng, Q Wang, Y Chao, O Rötzschke, EK Tan Parkinson&#039;s Disease-Specific Autoantibodies against the Neuroprotective Co-Chaperone STIP1 Cells, 2022-05-16;11(10):. 2022-05-16 [PMID: 35626686] (Bioassay, Human) Bioassay Human
M Lo, A Sharir, MD Paul, H Torosyan, C Agnew, A Li, C Neben, P Marangoni, L Xu, DR Raleigh, N Jura, OD Klein CNPY4 inhibits the Hedgehog pathway by modulating membrane sterol lipids Nature Communications, 2022-05-03;13(1):2407. 2022-05-03 [PMID: 35504891] (Bioassay, Mouse) Bioassay Mouse
A Moore, K Chinnaiya, DW Kim, S Brown, I Stewart, S Robins, GKC Dowsett, C Muir, M Travaglio, JE Lewis, F Ebling, S Blackshaw, A Furley, M Placzek Loss of Function of the Neural Cell Adhesion Molecule NrCAM Regulates Differentiation, Proliferation and Neurogenesis in Early Postnatal Hypothalamic Tanycytes Frontiers in Neuroscience, 2022-04-07;16(0):832961. 2022-04-07 [PMID: 35464310] (Bioassay, Human) Bioassay Human
L Torz, K Niss, S Lundh, JC Rekling, CD Quintana, SED Frazier, AJ Mercer, A Cornea, CV Bertelsen, MK Gerstenber, AMK Hansen, M Guldbrandt, J Lykkesfeld, LM John, JC Villaescus, N Petersen NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model International Journal of Molecular Sciences, 2022-03-17;23(6):. 2022-03-17 [PMID: 35328681] (Cell Culture, Human) Cell Culture Human
MM Capeling, S Huang, CJ Childs, JH Wu, YH Tsai, A Wu, N Garg, EM Holloway, N Sundaram, C Bouffi, M Helmrath, JR Spence Suspension culture promotes serosal mesothelial development in human intestinal organoids Cell Reports, 2022-02-15;38(7):110379. 2022-02-15 [PMID: 35172130] (Bioassay, Human) Bioassay Human
Show All 58 Publications.

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Gene Symbol SHH