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Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF

Images

 
1 μg/lane of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 22 kDa.
Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus (Catalog #1845-SH/CF) induces alkaline phosphatase production by the C3H10T1/2 mouse embryonic fibroblast cell line. The ED50 for this effect is 0.1‑0.4 ...read more
MALDI-TOF analysis of Recombinant Human Sonic Hedgehog/Shh (C24II), N-Terminus. The major peak corresponds to the calculated molecular mass, 19814 Da. The minor peak at 20029 is a matrix-associated artifact of the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF Summary

Additional Information
A New rh Shh is Available! C-term cholesterol, N-term fatty acid-modified; 250-fold activity!
Details of Functionality
Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun. 237:465. The ED50 for this effect is 0.1-0.4 µg/mL.
Source
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197 (Cys24Ile-Ile), with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
SHH
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
22 kDa, reducing conditions
Publications
Read Publications using
1845-SH/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS, and allow up to 24 hours at 2 to 8 °C for complete reconstitution.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Sonic Hedgehog/Shh (C24II) N-Terminus, CF

  • HHG1
  • HHG-1
  • HLP3
  • HPE3
  • MCOPCB5
  • MCOPCB5sonic hedgehog (Drosophila) homolog
  • Shh
  • ShhNC
  • SMMCI
  • SMMCIsonic hedgehog homolog (Drosophila)
  • sonic hedgehog homolog
  • sonic hedgehog protein
  • Sonic Hedgehog
  • TPT
  • TPTPS

Background

Sonic Hedgehog (Shh) is expressed in embryonic tissues that are critical for the patterning of the developing central nervous system, somite, and limb. It is also involved in whisker, hair, foregut, tooth, and bone development. Shh regulates neural and hematopoietic stem cell fate and is important for thymocyte differentiation and proliferation as well as T cell determination. In adult tissue Shh is associated with cancer development and tissue remodeling following injury (1-3). Human Shh encodes a 462 amino acid (aa) precursor protein that is autocatalytically processed to yield a non-glycosylated 19 kDa N-terminal fragment (Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at its
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
  1. Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
  2. Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
  3. Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
  4. Marigo, V. et al. (1995) Genomics 28:44.
  5. Zeng, X. et al. (2001) Nature 411:716.
  6. Feng, J. et al. (2004) Development 131:4357.
  7. Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
  8. Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
  9. Chen, M.-H. et al. (2004) Genes Dev. 18:641.
  10. Etheridge, L.A. et al. (2010) Development 137:133.
  11. Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
  12. Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
  13. Lewis, P.M. et al. (2001) Cell 105:599.
  14. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
  15. Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
  16. Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.

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Applications: Bioactivity

Publications for Sonic Hedgehog/Shh (1845-SH/CF)(48)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Pig, Xenopus.

We have publications tested in 4 applications: Bioassay, Cell Culture, In Vivo, Pull-Down Assay.


Filter By Application
Bioassay
(39)
Cell Culture
(5)
In Vivo
(1)
Pull-Down Assay
(1)
All Applications
Filter By Species
Human
(34)
Mouse
(7)
Rat
(2)
Bovine
(1)
Pig
(1)
Xenopus
(1)
All Species
Showing Publications 1 - 10 of 48. Show All 48 Publications.
Publications using 1845-SH/CF Applications Species
L Torz, K Niss, S Lundh, JC Rekling, CD Quintana, SED Frazier, AJ Mercer, A Cornea, CV Bertelsen, MK Gerstenber, AMK Hansen, M Guldbrandt, J Lykkesfeld, LM John, JC Villaescus, N Petersen NPFF Decreases Activity of Human Arcuate NPY Neurons: A Study in Embryonic-Stem-Cell-Derived Model International Journal of Molecular Sciences, 2022;23(6):. 2022 [PMID: 35328681] (Cell Culture, Human) Cell Culture Human
K Kerekes, M Trexler, L Bányai, L Patthy Wnt Inhibitory Factor 1 Binds to and Inhibits the Activity of Sonic Hedgehog Cells, 2021;10(12):. 2021 [PMID: 34944004] (Pull-Down Assay, Human) Pull-Down Assay Human
AM Earley, LF Burbulla, D Krainc, R Awatramani Identification of ASCL1 as a determinant for human iPSC-derived dopaminergic neurons Scientific Reports, 2021;11(1):22257. 2021 [PMID: 34782629] (Bioassay, Human) Bioassay Human
A Azatyan, S Zhang, A Darabi, P Siesjö, T Wang, PG Zaphiropou Circular RNAs in Hedgehog Signaling Activation and Hedgehog-Mediated Medulloblastoma Tumors Cancers, 2021;13(20):. 2021 [PMID: 34680287] (Cell Culture, Human) Cell Culture Human
K Nakai, T Shiga, R Yasuhara, AK Sarkar, Y Abe, S Nakamura, Y Hoashi, K Kotani, S Tatsumoto, H Ishikawa, Y Go, T Inoue, K Mishima, W Akamatsu, K Baba In vitro monitoring of HTR2A-positive neurons derived from human-induced pluripotent stem cells Scientific Reports, 2021;11(1):15437. 2021 [PMID: 34326453] (Bioassay, Human) Bioassay Human
JS Fleck, F Sanchís-Ca, Z He, M Santel, MJ Boyle, JG Camp, B Treutlein Resolving organoid brain region identities by mapping single-cell genomic data to reference atlases Cell Stem Cell, 2021;0(0):. 2021 [PMID: 33711282] (Bioassay, Human) Bioassay Human
L Cui, J Zheng, Q Zhao, JR Chen, H Liu, G Peng, Y Wu, C Chen, Q He, H Shi, S Yin, RA Friedman, Y Chen, MX Guan Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss JCI Insight, 2020;5(23):. 2020 [PMID: 33268592] (Bioassay, Human) Bioassay Human
CR Cochrane, V Vaghjiani, A Szczepny, WSN Jayasekara, A Gonzalez-R, K Kikuchi, GW McCaughan, A Burgess, DJ Gough, DN Watkins, JE Cain Trp53 and Rb1 regulate autophagy and ligand-dependent Hedgehog signaling J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32568216] (Bioassay, Mouse) Bioassay Mouse
C Yang, L Yong, C Liang, Y Li, Y Ma, F Wei, L Jiang, H Zhou, G He, X Pan, B Hai, J Wu, Y Xu, Z Liu, X Liu Genetic landscape and ligand-dependent activation of sonic hedgehog-Gli1 signaling in chordomas: a novel therapeutic target Oncogene, 2020;0(0):. 2020 [PMID: 32404987] (Cell Culture, Human) Cell Culture Human
B Ghaleh, J Thireau, O Cazorla, R Soleti, V Scheuerman, A Bizé, L Sambin, F Roubille, R Andriantsi, MC Martinez, A Lacampagne Cardioprotective effect of sonic hedgehog ligand in pig models of ischemia reperfusion Theranostics, 2020;10(9):4006-4016. 2020 [PMID: 32226535] (In Vivo, Pig) In Vivo Pig
Show All 48 Publications.

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Bioinformatics

Gene Symbol SHH
Uniprot