Recombinant Human Sirtuin 3/SIRT3 Protein, CF Summary
Details of Functionality
Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC (Catalog # ES016) in a coupled assay. The specific activity is >2 pmol/min/μg, as measured under the described conditions.
E. coli-derived human Sirtuin 3/SIRT3 protein Ser101-Lys399, with an N-terminal Met and 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<1.0 EU per 1 μg of the protein by the LAL method.
34 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
30-33 kDa, reducing conditions
Read Publication using 7488-DA in the following applications:
SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
SIR2-like protein 3
sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
sirtuin type 3
Sirtuin 3 (SIRT3) is a NAD+-dependent class III histone deacetylase. It is primarily compartmentalized to mitochondria (1) and activates mitochondrial target proteins, including ACSS1, IDH2 and GDH by deacetylating key lysine residues (2-4). SIRT3 plays an important role in regulating mitochondrial metabolism and energy production and thus has emerged as a potential therapeutic target to treat metabolic and neurological diseases (5). Polymorphisms in human SIRT3 have been linked to survivorship among the elderly, suggesting a possible involvement of SIRT3 in age-related phenomena (6).
North, B. J. et al. (2004) Genome Biology 5:224.
Schwer, B. et al. (2006) Proc. Natl. Acad. Sci. 103:11224.
Schlicker, C. et al. (2008) J. Mol. Biol. 382:790.
Ahn, B.-H. et al. (2008) Proc. Natl. Acad. Sci. 105:14447.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Review this Product
Be the first to review our Recombinant Human Sirtuin 3/SIRT3 Protein, CF and receive a gift card or discount.
PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.