Recombinant Human Siglec-15 Fc Chimera Protein, CF

Images

 
Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
When Neu5Ac(a2-6)GalNAc-PAA-biotin is immobilized onto a Streptavidin-coated plate, Recombinant Human Siglec-15 Fc Chimera (Catalog # 9227-SL) binds with an ED50 of150-900 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Siglec-15 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect is 1-10 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Siglec-15 protein
Human Siglec-15
(Phe20-Thr263)
Accession # Q6ZMC9
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Phe20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
53-64 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, NaCl, CHAPS and Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 200 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Siglec-15 Fc Chimera Protein, CF

  • CD33 antigen-like 3SIGLEC-15
  • CD33 molecule-like 3
  • CD33L3
  • HsT1361
  • sialic acid binding Ig-like lectin 15
  • sialic acid-binding Ig-like lectin 15
  • Siglec15
  • Siglec-15

Background

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).
  1. Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
  2. Angata, T. et al. (2007) Glycobiology 17:838.
  3. Takamiya, R. et al. (2013) Glycobiology 23:178.
  4. Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
  5. Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
  6. Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

Customers Who Viewed This Item Also Viewed...

NB600-534
Species: Hu, Mu, Po, V-Vi
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, RIA
NBP1-32945
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
MAB5240
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
NB100-56508
Species: Hu, Mu, Rt, Av
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
MAB97861
Species: Hu
Applications: Flow, IHC, IHC-P
NBP2-22377
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-46125
Species: Hu, Mu, Rt, Ca, Pm
Applications: WB, ICC/IF
MAB10721
Species: Hu
Applications: Flow, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NB100-56732
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF
H00057410-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, S-ELISA
NBP1-98585
Species: Rt
Applications: WB
NBP2-92897
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
9227-SL
Species: Hu
Applications: Bioactivity

Publications for Siglec-15 (9227-SL) (0)

There are no publications for Siglec-15 (9227-SL).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Siglec-15 (9227-SL) (0)

There are no reviews for Siglec-15 (9227-SL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Siglec-15 (9227-SL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Siglec-15 Products

Diseases for Siglec-15 (9227-SL)

Discover more about diseases related to Siglec-15 (9227-SL).
 

Pathways for Siglec-15 (9227-SL)

View related products by pathway.

Blogs on Siglec-15

There are no specific blogs for Siglec-15, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Siglec-15 Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot