Recombinant Human Proinsulin Protein, CF

Images

 
There are currently no images for Proinsulin (1336-PN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Proinsulin Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.15-0.75 μg/mL.
Source
E. coli-derived human Proinsulin protein
Phe25-Asn110, with an N-terminal Met, 6-His tag and Lys
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
INS
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Publications
Read Publications using
1336-PN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Proinsulin Protein, CF

  • IDDM2
  • ILPR
  • insulin
  • IRDN
  • MODY10
  • Proinsulin

Background

Proinsulin is synthesized as a single chain, 110 amino acid (aa) preproprecursor that contains a 24 aa signal sequence and an 86 aa proinsulin propeptide. Following removal of the signal peptide, the proinsulin peptide undergoes further proteolysis to generate mature insulin, a 51 aa disulfide-linked dimer that consists of a 30 aa B chain (aa 25 - 54) bound to a 21 aa A chain (aa 90 - 110). The 34 aa intervening peptide (aa 55 - 89) that connects the B and A chains is termed the C-peptide. Human proinsulin shares 84% and 80% aa sequence identity with rat and bovine proinsulin, respectively. Most of the sequence variation between species occurs in the region of the C-peptide (1). This peptide generates a structural conformation that allows for the correct formation of the intrachain disulphide bonds (1). Insulin is a molecule that facilitates the cellular uptake of glucose. This is accomplished by regulating the appearance of membrane glucose transporters. Low insulin levels or lack of insulin are associated with type 2 and type 1 diabetes mellitus, respectively. These conditions are associated with an increased risk for microvascular complications such as retinopathy, nephropathy, and peripheral neuropathy (3). Proinsulin also circulates, but its physiologic role is less well understood. It does possess about 25% of the activity of mature insulin, but it would seem unlikely to be a natural substitute for insulin (4). In type 2 diabetes, an elevated proinsulin to insulin ratio in the circulation is a well-known abnormality (5 - 9). Perhaps this abnormality represents either compromised proteolytic processing or a general inability to process increased levels of insulin precursor (5). In any event, proinsulin will stimulate amylin secretion by beta -cells, and amyloid formation in pancreatic islets that promotes decreased beta cell function (10). Studies also suggest that fasting serum proinsulin may be a better predictor of future type 2 diabetes than fasting insulin levels in obese children (11).

  1. Bell, G.I. et al. (1980) Nature 284:26.
  2. Barbetti, F. et al. (1990) J. Clin. Endocrinol. Metab. 71:164.
  3. Forst, T. et al. (2008) Exp. Diabetes Res. 2008:176245.
  4. Steffes, M.W. et al. (2003) Diabetes Care 26:832.
  5. Roder, M.E. et al. (1999) Diabetes Care 22:609.
  6. Porte, D. Jr. (1991) Diabetes 40:166.
  7. Gordon, P. et al. (1974) Diabetologia 34:483.
  8. Saad, M.F. et al. (1990) J. Clin. Endocrinol. Metab. 70:1247.
  9. Roder, M.E. et al. (1995) J. Clin. Endocrinol. Metab. 80:2359.
  10. Dworacka, M. et al. (2006) Int. J. Clin. Pharmacol. Ther. 44:14.
  11. Kamoda, T. et al. (2006) Diabetes Obes. Metab. 8:192.

Customers Who Viewed This Item Also Viewed...

MAB1249
Species: Hu, Mu
Applications: CyTOF-ready, ICC, ICFlow
AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
NBP2-12793
Species: Hu, Mu
Applications: WB, IP
AF1067
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, Neut
NB100-64650
Species: Hu, Mu, Rt, Po, GP, Ze
Applications: IHC, IHC-Fr, IHC-P
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-1533
Species: Hu, Mu, Po, Sh
Applications: WB, ICC/IF, IHC, IHC-P, PEP-ELISA
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-02142
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P

Publications for Proinsulin (1336-PN)(6)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 3 applications: Bioassay, ELISA Standard, In Vivo.


Filter By Application
Bioassay
(4)
ELISA Standard
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(1)
N/A
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 1336-PN Applications Species
YJ Hsieh, KY Chien, IF Yang, IN Lee, CC Wu, TY Huang, JS Yu Oxidation of protein-bound methionine in Photofrin-photodynamic therapy-treated human tumor cells explored by methionine-containing peptide enrichment and quantitative proteomics approach Sci Rep, 2017;7(1):1370. 2017 [PMID: 28465586] (Bioassay, N/A) Bioassay N/A
Farino Z, Morgenstern T, Vallaghe J, Gregor N, Donthamsetti P, Harris P, Pierre N, Freyberg R, Charrier-Savournin F, Javitch J, Freyberg Z Development of a Rapid Insulin Assay by Homogenous Time-Resolved Fluorescence. PLoS ONE, 2016;11(2):e0148684. 2016 [PMID: 26849707] (ELISA Standard, Rat) ELISA Standard Rat
Rulifson I, Cao P, Miao L, Kopecky D, Huang L, White R, Samayoa K, Gardner J, Wu X, Chen K, Tsuruda T, Homann O, Baribault H, Yamane H, Carlson T, Wiltzius J, Li Y Identification of Human Islet Amyloid Polypeptide as a BACE2 Substrate. PLoS ONE, 2016;11(2):e0147254. 2016 [PMID: 26840340] (Bioassay, Human) Bioassay Human
Mbongue J, Nicholas D, Zhang K, Kim N, Hamilton B, Larios M, Zhang G, Umezawa K, Firek A, Langridge W Induction of indoleamine 2, 3-dioxygenase in human dendritic cells by a cholera toxin B subunit-proinsulin vaccine. PLoS ONE, 2015;10(2):e0118562. 2015 [PMID: 25714914] (Bioassay, Human) Bioassay Human
Nardi F, Lipina C, Magill D, Hage Hassan R, Hajduch E, Gray A, Hundal H Enhanced insulin sensitivity associated with provision of mono and polyunsaturated fatty acids in skeletal muscle cells involves counter modulation of PP2A. PLoS ONE, 0;9(3):e92255. 0 [PMID: 24632852] (Bioassay, Human) Bioassay Human
Wang, Yan, Shao, Juntang, Zaro, Jennica, Shen, Wei-Chia Proinsulin-transferrin fusion protein as a novel long-acting insulin analog for the inhibition of hepatic glucose production. Diabetes, 2014;63(5):1779-88. 2014 [PMID: 24353179] (In Vivo, Mouse) In Vivo Mouse

Reviews for Proinsulin (1336-PN) (0)

There are no reviews for Proinsulin (1336-PN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Proinsulin (1336-PN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Proinsulin (1336-PN)

Discover related pathways, diseases and genes to Proinsulin (1336-PN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Proinsulin (1336-PN)

Discover more about diseases related to Proinsulin (1336-PN).
 

Pathways for Proinsulin (1336-PN)

View related products by pathway.

PTMs for Proinsulin (1336-PN)

Learn more about PTMs related to Proinsulin (1336-PN).

Blogs on Proinsulin

There are no specific blogs for Proinsulin, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Albumin Antibody
NB600-41532

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Proinsulin Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol INS
Entrez
Uniprot