Recombinant Human Plexin A3 His-tag Protein, CF

2 μg/lane of Recombinant Human Plexin A3 His-tag Protein (10519-PA) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 135-160 kDa.

• Reactivity: Hu
• Applications: NULL
• Format: Carrier-Free

Recombinant Human Plexin A3 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Plexin A3 protein Asn20-Ala1220, with a C-terminal 6-His tag
• Accession #: NP_059984.3
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • N/A
• Theoretical MW: 133 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 135-160 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Plexin A3 His-tag Protein, CF

• HSSEXGENE
• Plexin A3
• plexin-4
• plexin-A3
• Plxn3
• PLXN4
• PLXNA3
• Semaphorin receptor SEX
• Sex chromosome X transmembrane protein of HGF receptor family 3
• SEX
• SEXPLEXIN-A3,6.3
• XAP-6

Background

Plexin A3, also known as Plexin-4, is a member of the large Plexin family of conserved transmembrane receptors that mediate Semaphorin functions (1, 2). There are four classes plexins (A, B, C, and D) and they share a similar domain structure: An extracellular domain (ECD) that binds the semaphorin ligand, a single transmembrane domain and an approximately 600 residue-long cytoplasmic region (2). The ECD of mature, human plexinA-3 consists of a Sema domain and four IPT (Ig-like, plexins, transcription factors) domains and shares 93% amino acid identity with both mouse and rat PlexinA-3. Plexin A3 is a coreceptor for class 3 semaphorins and plays an important role in axon guidance in the developing nervous system and regulates the migration of sympathetic neurons (3, 4). Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin (4). Plexin A3 is required for normal dendrite spine morphology in pyramidal neurons and may play a role in regulating semaphorin-mediated programmed cell death in the developing nervous system (5). The cytoplasmic domain of plexinA-3 is required for the activation of down-stream signaling events in the cytoplasm (6).

1. Pascoe, H.G. et al. (2015) Prog Biophys Mol Biol. 118(3):161.
2. Tamagnone L et al. (1999) Cell. 99:71.
3. Alto, L. T. et al. (2017) Methods Mol Biol. 1493:1.
4. Yaron, A. et al. (2005) Neuron. 45:513.
5. Cheng, H. J. et al. (2001) Neuron. 32:249.
6. He H. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:15610.

Bioinformatics

• Uniprot:
  • NP_059984.3()