Plexin C1 Antibody [Unconjugated]

Western blot shows lysates of mouse thymus tissue and embryonic mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin C1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Mouse splenocytes were stained with Sheep Anti-Mouse Plexin C1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5375, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

Plexin C1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurites in the developing midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

Sema7A induces the retraction of GnRH neurites through the PlexinC1 transduction pathway and Rap1 inactivation.(a) Representative images of semaphorin-induced neurite retraction in GnV3 neurons transfected with an empty vector (mock), a construct encoding a PlexinC1 short hairpin RNA (GnV3 shRNA PlexinC1) or a Rap1-V12 plasmid encoding a constitutively active form of Rap1. One day following Sema7A treatment (250 ng ml−1), cells were labelled for F-actin (red) to visualize cytoskeletal changes. (b) Quantitative analysis of neurite length in GnV3 cells under different treatment conditions (n=5 independent experiments, n=303 mock-transfected control cells, n=303 mock-transfected cells after Sema7A treatment, unpaired Student’s t-test, ***P0.05; n=131 Rap1-V12-transfected cells, n=115 Rap1-V12-transfected cells+Sema7A, unpaired Student’s t-test, P>0.05). (c) Immunoblotting for markers indicated using transfected GnV3 cells. (d–f) Saline, Sema7A or Sema7A+PlexinC1 was infused (0.2 μg μl−1, 0.5 μl h−1 for 7 days) by stereotaxic implantation of a 28-gauge infusion cannula connected to a subcutaneously implanted mini-osmotic pump in the ME of cycling female rats. Representative oestrous cycle profiles showing the disruption of oestrous cyclicity by the infusion of Sema7A but not of PBS into the ME. Infusion was started on day 9 (downward arrow) and ended 7 days later (upward arrow), when pump contents were exhausted. Die, Diestrus; Es, estrus; Pro, proestrus. (g) Quantitative analysis of alterations in ovarian cyclicity (percentage of time in diestrus) caused by PBS, Sema7A or Sema7A+soluble PlexinC1 infusion into the rat ME (n=6 animals per group, Kruskal–Wallis test, **P<0.005). Scale bar, (a) 10 μm. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25721933), licensed under a CC-BY license. Not internally tested by R&D Systems.

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

PlexinC1 expression in the subgranular zone is largely confined to early progenitors.(a–f) Double immunohistochemistry for plexinC1 and Ki-67 (a), glial fibrillary acidic protein (GFAP; b), sex determining region Y-box 2 (Sox2; c), t-box brain 2 (TBR2; d), doublecortin (DCX; e) or neuronal nuclei (NeuN; f) in sections of the adult mouse dentate gyrus (DG). The majority of plexinC1-positive cells express Ki-67, GFAP and Sox2 but not TBR2, DCX or NeuN. Arrowheads indicate cells expressing specific markers. Arrows indicate plexinC1-positive cells not expressing the indicated marker. (g,h) Quantification of the fraction of cells expressing a specific marker that also express plexinC1 (g) or the fraction of plexinC1-positive cells that also express the indicated marker protein (h). Data are presented as means±s.e.m. n≥3 (mice). (i) Schematic representation of lineage-marker and plexinC1 expression in cell lineage subtypes during neuronal differentiation in the adult DG. Ki-67 is a marker for proliferating cells during all active phases of the cell cycle. PlexinC1 is expressed in GFAP-positive and Sox2-positive radial glia-like cells (RGLs) and early intermediate progenitor cells (IPCs). Ki-67-positive proliferating cells express plexinC1. In contrast, only a small fraction of TBR2-, DCX- or NeuN-positive intermediate progenitor cells and (immature) granule cells express plexinC1. Scale bars: 10 μm. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms14666), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Plexin C1 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin C1
  Ala35-Thr950
  Accession # Q9QZC2
• Specificity: Detects mouse Plexin C1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human Plexin C1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: PLXNC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin C1 Antibody [Unconjugated]

• CD232 antigen
• CD232
• plexin (semaphorin receptor)
• Plexin C1
• PLXNC1
• PLXN-C1
• receptor for viral semaphorin protein
• receptor for virally-encoded semaphorin
• VESPR
• VESPRplexin-C1
• Virus-encoded semaphorin protein receptor

Background

Plexin C1 (also VESPR and CD232) is a 200‑210 kDa member of the plexin C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells, neutrophils and embryonic neuroendocrine neurons of the hyypothalamus. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature mouse Plexin C1 is 1540 amino acids (aa) in length. It contains a 916 aa extracellular domain that shows one Sema-domain (aa 35‑452) and a 603 aa cytoplasmic tail. There are two potential splice variants that show a five and 67 aa substitution for aa 861‑870 and 1206‑1574, respectively. Over aa 35‑950, mouse Plexin C1 is 95% and 85% aa identical to rat and human Plexin C1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Plexin C1 Antibody (AF5375)(9)

Publications using AF5375

• Bernard, A;Eggstein, C;Tang, L;Keller, M;Körner, A;Mirakaj, V;Rosenberger, P; Plexin C1 influences immune response to intracellular LPS and survival in murine sepsis Journal of biomedical science 2024-08-21 [PMID: 39169397] (Western Blot, Mouse)
• N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy Cell Reports, 2023-03-21;42(4):112295. 2023-03-21 [PMID: 36947543] (IHC, Mouse)
• Gioele W. Albisetti, Robert P. Ganley, Francesca Pietrafesa, Karolina Werynska, Marília Magalhaes de Sousa, Rebecca Sipione et al. Inhibitory Kcnip2 neurons of the spinal dorsal horn control behavioral sensitivity to environmental cold Neuron 2023-01-04 [PMID: 36323322]
• N Vilallongu, J Schaeffer, AM Hesse, C Delpech, B Blot, A Paccard, E Plissonnie, B Excoffier, Y Couté, S Belin, H Nawabi Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system Nature Communications, 2022-10-13;13(1):6040. 2022-10-13 [PMID: 36229455] (IHC, Mouse)
• A Chabrat, G Brisson, H Doucet-Bea, C Salesse, M Schaan Pro, A Dovonou, C Akitegetse, J Charest, S Lemstra, D Côté, RJ Pasterkamp, MI Abrudan, E Metzakopia, SL Ang, M Lévesque Transcriptional repression of Plxnc1 by Lmx1a and Lmx1b directs topographic dopaminergic circuit formation Nat Commun, 2017-10-16;8(1):933. 10/16/2017 [PMID: 29038581]
• H Lee, LJ Macpherson, CA Parada, CS Zuker, NJP Ryba Rewiring the taste system Nature, 2017-08-09;548(7667):330-333. 2017-08-09 [PMID: 28792937] (IHC, Mouse)
• Minlong Zhang, Xue Yan, Wei Liu, Ruilin Sun, Yonghong Xie, Faguang Jin Endothelial semaphorin 7A promotes seawater aspiration-induced acute lung injury through plexin C1 and beta 1 integrin Molecular Medicine Reports 2017-10-01 [PMID: 28765893]
• Parkash J, Messina A, Langlet F, Cimino I, Loyens A, Mazur D, Gallet S, Balland E, Malone S, Pralong F, Cagnoni G, Schellino R, De Marchis S, Mazzone M, Pasterkamp R, Tamagnone L, Prevot V, Giacobini P Semaphorin7A regulates neuroglial plasticity in the adult hypothalamic median eminence. Nat Commun, 2015-02-27;6(0):6385. 2/27/2015 [PMID: 25721933]
• Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. Nat Neurosci, 2014-11-24;18(1):145-53. 2014-11-24 [PMID: 25420068] (IHC, Mouse)

Bioinformatics

• Gene Symbol: PLXNC1
• Uniprot:
  • Q9QZC2()