Recombinant Human PDGF-BB Biotinylated Protein, CF

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Both Biotinylated Recombinant Human PDGF‑BB (Catalog # BT220/CF) and unlabeled Recombinant Human PDGF‑BB (Catalog # 220‑BB) stimulate NR6R‑3T3 mouse fibroblast cell proliferation. The ED50 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human PDGF-BB Biotinylated Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is 1.5-6 ng/mL.
Source
E. coli-derived human PDGF-BB protein
Ser82-Thr190
Accession #
Structure / Form
Disulfide-linked homodimer, biotinylated protein via amines
Protein/Peptide Type
Recombinant Proteins
Gene
PDGFB
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12 kDa (monomer, unlabeled).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12 kDa, reducing conditions
Publications
Read Publication using
BT220/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF-BB Biotinylated Protein, CF

  • PDGFBB
  • PDGF-BB

Background

Platelet-Derived Growth Factor (PDGF)-BB is synthesized as a 35 kDa, 241 amino acid (aa) prepro-precurser. It contains a signal peptide, an N-terminal prodomain, a mature region, and a C-terminal prodomain (1-4). The propercursor is initially dimerized and then intracellulary processed twice. The N-terminal prodomain is cleaved first, followed by cleavage of the C-terminal prodomain. The resulting mature region is 16-17 kDa in size (or 29-32 kDa as a homodimer) (4). Mature human PDGF-B shares 89% aa sequence identity with mouse mature PDGF-B. PDGF-BB is expressed by hepatocytes and nonresorbing osteoclasts, generating osteoblasts and bone formation (4, 5). It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization (6, 7). The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta (8, 9). PDGF-BB has been shown to bind the alpha alpha homodimer, alpha beta heterodimer, and the beta beta  homodimer in vitro, and act through the beta beta homodimer in vivo (8, 10).
  1. Rao, C.D. et al. (1986) Proc. Natl. Acad. Sci. USA 83:2392.
  2. Kaetzel, D.M. et al. (1996) Biochim. Biophys. Acta. 1298:250.
  3. Ostman, A. et al. (1992) J. Cell Biol. 118:509.
  4. Siegfried, G. et al. (2005) Oncogene 24:6925.
  5. Kreja, L. et al. (2010) J. Cell. Biochem. 109:347.
  6. van Steensel, L. et al. (2012) J. Clin. Endocrinol. Metab. 97:E400.
  7. Barrientos, S. et al. (2008) Wound Repair Regen. 16:585.
  8. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  9. Heldin, C.H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  10. Li, X. and U. Eriksson (2003) Cytokine Growth Factor Rev. 14:91.

Publications for PDGF-BB (BT220/CF)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol PDGFB
Entrez
Uniprot