Recombinant Human OSCAR Fc Chimera Protein, CF

When Bovine Collagen I is coated at 10 µg/mL, RecombinantHuman OSCAR Fc Chimera binds with an ED50 of 1-8 ng/mL.

2 μg/lane of Recombinant Human OSCAR was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 61-70 kDa and 120-140 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human OSCAR Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Bovine Collagen I is coated at 10 µg/mL, Recombinant Human OSCAR Fc Chimera binds with an ED₅₀ of 1-8 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human OSCAR protein
  

  Human OSCAR (Asp19-Asn229) Accession # NP_573399	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_573399
• N-terminal Sequence: Asp19
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 61-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human OSCAR Fc Chimera Protein, CF

• hOSCAR
• MGC33613
• OSCAR
• osteoclast associated receptor OSCAR-S1
• osteoclast associated receptor OSCAR-S2
• osteoclast associated, immunoglobulin-like receptor
• osteoclast-associated immunoglobulin-like receptor
• Osteoclast-associated receptor
• PIGR3
• PIgR-3
• Poly-Ig receptor 3
• Polymeric immunoglobulin receptor 3

Background

OSCAR (Osteoclast-associated immunoglobulin-like receptor), also known as PIgR-3 (polymeric immunoglobulin-like receptor), is a type I transmembrane protein of the leukocyte receptor complex (LRC) family (1). The mature human OSCAR includes an extracellular domain (ECD) with two Ig domains homology to PIR family proteins, a transmembrane region, and a cytoplasmic domain. The ECD of human OSCAR shares 77% and 72% amino acid sequence identity with the ECD of mouse and rat OSCAR, respectively. OSCAR is specifically expressed by pre-osteoclasts and signals via FcR gamma (2). OSCAR co-stimulates one of the major FcR gamma -associated pathways required for osteoclastogenesis in vivo (2). Collagen type I and collagen type II serve as ligands for OSCAR (3). OSCAR binds to specific motifs within fibrillary collagens in the extracellular matrix (ECM) (2). OSCAR promoted osteoclastogenesis in vivo, and its binding to the collagen motif led to signaling that increased numbers of osteoclasts in culture (2). OSCAR contains two immunoglobulin-like domains, D1 and D2. Direct binding assays on OSCAR molecules showed that D2, but not D1, is critical for collagen binding (4). OSCAR may contribute to the pathogenesis and severity of a number of diseases including osteoporosis, atherosclerosis, chronic obstructive pulmonary disease and rheumatoid arthritis (4).

1. Kim N. el al. (2002) J. Exp. Med. 195:201.
2. Barrow, A.D. et al. (2011) J. Clin. Invest. 121:3505.
3. Shultz, H.S. et al. (2016) Eur. J. Immunol. 46:952.
4. Zhou, L. et al. (2016) Blood 127:529.

Publications for OSCAR (9955-OS)(1)

Publication using 9955-OS

• Vitale, M;Ligorio, C;Richardson, SM;Hoyland, JA;Bella, J; Collagen-like Osteoclast-Associated Receptor (OSCAR)-Binding Motifs Show a Co-Stimulatory Effect on Osteoclastogenesis in a Peptide Hydrogel System International journal of molecular sciences 2023-12-28 [PMID: 38203618] (Surface Plasmon Resonance (SPR), N/A)

Bioinformatics

• Uniprot:
  • NP_573399()