ILT3/CD85k/LILRB4 Antibody (293623) [Unconjugated]

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human IL‑3 R alpha /CD123 PE‑conjugated Monoclonal Antibody (Catalog # FAB301P) and either (A) Mouse Anti-Human LILRB4/CD85k/ILT3 Monoclonal Antibody (Catalog # MAB24251) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready
• Clone: 293623
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ILT3/CD85k/LILRB4 Antibody (293623) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human LILRB4/CD85k/ILT3
  Pro17-His257
  Accession # ABM86208
• Specificity: Detects human LILRB4/CD85k/ILT3 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with with recombinant human (rh) ILT5 is observed. No cross-reactivity with rhILT1, rhILT2, rhILT4, rhILT7, rhILT11, rhLIR-6 or rhLIR-8 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LILRB1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ILT3/CD85k/LILRB4 Antibody (293623) [Unconjugated]

• CD85 antigen-like family member K
• CD85k antigen
• CD85k
• HM18
• ILT3
• ILT-3
• ILT3CD85K
• leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains)
• LILRB4
• LIR5
• LIR5LILRB5
• LIR-5subfamily B, member 4
• member 4

Background

Immunoglobulin-Like Transcript 3 (ILT3), also known as Leukocyte Immunoglobulin-Like Receptor subfamily B member 4 (LILRB4) and Leukocyte Immunoglobulin-like Receptor 5 (LIR-5), is a type I membrane protein that functions as a receptor for class I MHC antigens. It contains cytoplasmic ITIM motifs and is involved in the down-regulation of immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ILT3/CD85k/LILRB4 Antibody (MAB24251)(3)

Publications using MAB24251

• S Tomi?, N Ili?, V Kokol, A Gruden-Mov, D Mihajlovi?, M Beki?, L Sofroni?-M, M ?oli?, D Vu?evi? Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells Int J Nanomedicine, 2018;13(0):6941-6960. 2018 [PMID: 30464452] (Neutralization, Human)
• Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli. J. Immunol., 2011;186(12):6701-9. 2011 [PMID: 21543643] (Flow Cytometry, Human)
• Wenink MH, Santegoets KC, Roelofs MF, Huijbens R, Koenen HJ, van Beek R, Joosten I, Meyer-Wentrup F, Mathsson L, Ronnelid J, Adema GJ, Bonvini E, Koenig S, van den Berg WB, van Riel PL, Radstake TR The inhibitory Fc gamma IIb receptor dampens TLR4-mediated immune responses and is selectively up-regulated on dendritic cells from rheumatoid arthritis patients with quiescent disease. J. Immunol., 2009;183(7):4509-20. 2009 [PMID: 19734236] (Flow Cytometry, Human)

Review for ILT3/CD85k/LILRB4 Antibody (MAB24251) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Lei Nie WB Human 04/03/2020

Summary

• Application: Western Blot
• Sample Tested: Caco-2 cell line,HUH7
• Species: Human
• Lot: WNX011809A

Bioinformatics

• Gene Symbol: LILRB1
• Uniprot:
  • ABM86208()