Recombinant Human NT-3 Protein, CF

Images

 
1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human NT-3 (Catalog #267‑N3/CF) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human NT-3 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.
The specific activity of recombinant human NT-3 is approximately 2082 U/μg, which is calibrated against Recombinant Human NT-3 Standard (NIBSC code: 98/718).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived
Tyr139-Thr257 (K196R)
Accession #
N-terminal Sequence
Tyr139
Protein/Peptide Type
Recombinant Proteins
Gene
NTF3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
267-N3/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein, CF

  • 3-nucleotidase
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

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Publications for NT-3 (267-N3/CF)(27)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, N/A, Primate, Primate - Callitrix jacchus (Common Marmoset).

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, Neural Differentiation.


Filter By Application
Bioassay
(23)
ELISA Standard
(1)
In Vivo
(1)
Neural Differentiation
(1)
All Applications
Filter By Species
Human
(14)
Mouse
(7)
Rat
(2)
Canine
(1)
N/A
(1)
Primate
(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using 267-N3/CF Applications Species
M Zhang, MH Jiang, DW Kim, W Ahn, E Chung, Y Son, G Chi Comparative Analysis of the Cell Fates of Induced Schwann Cells from Subcutaneous Fat Tissue and Na�ve Schwann Cells in the Sciatic Nerve Injury Model Biomed Res Int, 2017;2017(0):1252851. 2017 [PMID: 28713821] (Bioassay, Rat) Bioassay Rat
J Drouin-Oue, S Lau, PL Brattås, D Rylander O, K Pircs, DA Grassi, LM Collins, R Vuono, A Andersson, G Westergren, C Graff, L Minthon, H Toresson, RA Barker, J Jakobsson, M Parmar REST suppression mediates neural conversion of adult human fibroblasts via microRNA-dependent and -independent pathways EMBO Mol Med, 2017;9(8):1117-1131. 2017 [PMID: 28646119] (Bioassay, Human) Bioassay Human
W Wu, XL Wu, YQ Ji, Z Gao Differentiation of nestin?negative human hair follicle outer root sheath cells into neurons in�vitro Mol Med Rep, 2017;0(0):. 2017 [PMID: 28534946] (Bioassay, Human) Bioassay Human
S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493. 2017 [PMID: 28270618] (Bioassay, Mouse) Bioassay Mouse
SK Goparaju, K Kohda, K Ibata, A Soma, Y Nakatake, T Akiyama, S Wakabayash, M Matsushita, M Sakota, H Kimura, M Yuzaki, SB Ko, MS Ko Rapid differentiation of human pluripotent stem cells into functional neurons by mRNAs encoding transcription factors Sci Rep, 2017;7(0):42367. 2017 [PMID: 28205555] (Bioassay, Human) Bioassay Human
T Yamashita, Y Miyamoto, Y Bando, T Ono, S Kobayashi, A Doi, T Araki, Y Kato, T Shirakawa, Y Suzuki, J Yamauchi, S Yoshida, N Sato Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells PLoS ONE, 2017;12(2):e0171947. 2017 [PMID: 28192470] (Bioassay, Primate) Bioassay Primate
Small molecules increase direct neural conversion of human fibroblasts Sci Rep, 2016;6(0):38290. 2016 [PMID: 27917895] (Bioassay, Human) Bioassay Human
Synapse Formation in Monosynaptic Sensory-Motor Connections Is Regulated by Presynaptic Rho GTPase Cdc42 J Neurosci, 2016;36(21):5724-35. 2016 [PMID: 27225763] (Bioassay, Mouse) Bioassay Mouse
Sequential Differentiation of Embryonic Stem Cells into Neural Epithelial-Like Stem Cells and Oligodendrocyte Progenitor Cells PLoS ONE, 2016;11(5):e0155227. 2016 [PMID: 27192219] (Bioassay, Mouse) Bioassay Mouse
Neurotrophin-3 Regulates Synapse Development by Modulating TrkC-PTP? Synaptic Adhesion and Intracellular Signaling Pathways J Neurosci, 2016;36(17):4816-31. 2016 [PMID: 27122038] (Bioassay, Human) Bioassay Human
Show All 27 Publications.

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Bioinformatics

Gene Symbol NTF3
Entrez
Uniprot