Recombinant Human NT-3 Protein, CF


1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human NT-3 (Catalog #267‑N3/CF) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.

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Recombinant Human NT-3 Protein, CF Summary

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Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 4-40 ng/mL.
The specific activity of recombinant human NT-3 is approximately 2082 U/μg, which is calibrated against Recombinant Human NT-3 Standard (NIBSC code: 98/718).
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-3 protein
Tyr139-Thr257 (K196R)
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
267-N3/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein, CF

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3


Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

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Publications for NT-3 (267-N3/CF)(49)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Primate, Primate - Callitrix jacchus (Common Marmoset).

We have publications tested in 6 applications: Bioassay, CAR-T, Cell Culture, Differentiation, ELISA (Standard), In Vivo.

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Primate - Callitrix jacchus (Common Marmoset)
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Showing Publications 1 - 10 of 49. Show All 49 Publications.
Publications using 267-N3/CF Applications Species
H Jiang, F Niu, Y Zheng, Y Xu CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating &amp;beta?amyloid metabolism?associated enzymes Molecular Medicine Reports, 2021;23(4):1-12. 2021 [PMID: 33604684] (CAR-T, Mouse) CAR-T Mouse
J Giacomoni, A Bruzelius, CA Stamouli, D Rylander O Direct Conversion of Human Stem Cell-Derived Glial Progenitor Cells into GABAergic Interneurons Cells, 2020;9(11):. 2020 [PMID: 33182669] (Bioassay, Human) Bioassay Human
KC Allan, LR Hu, MA Scavuzzo, AR Morton, AS Gevorgyan, EF Cohn, BLL Clayton, IR Bederman, S Hung, CF Bartels, M Madhavan, PJ Tesar Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33091368] (Bioassay, Mouse) Bioassay Mouse
KM Prautsch, A Schmidt, V Paradiso, DJ Schaefer, R Guzman, DF Kalbermatt, S Madduri Modulation of Human Adipose Stem Cells&#039; Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro Cells, 2020;9(9):. 2020 [PMID: 32839392] (Cell Culture, Human) Cell Culture Human
N Michel, P Narayanan, O Shomroni, M Schmidt Maturational Changes in Mouse Cutaneous Touch and Piezo2-Mediated Mechanotransduction Cell Rep, 2020;32(3):107912. 2020 [PMID: 32697985] (Bioassay, Mouse) Bioassay Mouse
N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay Variation of Human Neural Stem Cells Generating Organizer States In�Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 2020;31(5):107599. 2020 [PMID: 32375049] (Cell Culture, Mouse) Cell Culture Mouse
EE Burke, JG Chenoweth, JH Shin, L Collado-To, SK Kim, N Micali, Y Wang, C Colantuoni, RE Straub, DJ Hoeppner, HY Chen, A Sellers, K Shibbani, GR Hamersky, M Diaz Busta, BN Phan, WS Ulrich, C Valencia, A Jaishankar, AJ Price, A Rajpurohit, SA Semick, RW Bürli, JC Barrow, DJ Hiler, SC Page, K Martinowic, TM Hyde, JE Kleinman, KF Berman, JA Apud, AJ Cross, NJ Brandon, DR Weinberger, BJ Maher, RDG McKay, AE Jaffe Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs Nat Commun, 2020;11(1):462. 2020 [PMID: 31974374] (Cell Culture, Human) Cell Culture Human
AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020;30(3):932-946.e7. 2020 [PMID: 31968264] (Differentiation, Human) Differentiation Human
M Matjusaiti, LJ Wagstaff, A Martella, B Baranowski, C Blin, S Gogolok, A Williams, SM Pollard Reprogramming of Fibroblasts to Oligodendrocyte Progenitor-like Cells Using CRISPR/Cas9-Based Synthetic Transcription Factors Stem Cell Reports, 2019;13(6):1053-1067. 2019 [PMID: 31708478] (Cell Culture, Mouse) Cell Culture Mouse
CY Lin, M Yoshida, LT Li, A Ikenaka, S Oshima, K Nakagawa, H Sakurai, E Matsui, T Nakahata, MK Saito iPSC-derived functional human neuromuscular junctions model the pathophysiology of neuromuscular diseases JCI Insight, 2019;4(18):. 2019 [PMID: 31534050] (Bioassay, Human) Bioassay Human
Show All 49 Publications.

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Gene Symbol NTF3