Recombinant Human NT-3 Protein, CF

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1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Recombinant Human NT-3 (Catalog #267‑N3/CF) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is 1-10 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human NT-3 Protein, CF Summary

Additional Information
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Details of Functionality
Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 4-40 ng/mL.
The specific activity of recombinant human NT-3 is approximately 2082 U/μg, which is calibrated against Recombinant Human NT-3 Standard (NIBSC code: 98/718).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human NT-3 protein
Tyr139-Thr257 (K196R)
Accession #
N-terminal Sequence
Tyr139
Protein/Peptide Type
Recombinant Proteins
Gene
NTF3
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
267-N3/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human NT-3 Protein, CF

  • HDNF
  • MGC129711
  • Nerve growth factor 2
  • Neurotrophic factor
  • neurotrophin 3
  • neurotrophin-3
  • NGF2
  • NGF-2
  • NT3
  • NT-3
  • NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

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Publications for NT-3 (267-N3/CF)(66)

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Canine, Chicken, N/A, Primate, Primate - Callitrix jacchus (Common Marmoset), Transgenic Mouse.

We have publications tested in 6 applications: Bioassay, CAR-T, Cell Culture, Differentiation, ELISA (Standard), In Vivo.


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Bioassay
(54)
CAR-T
(1)
Cell Culture
(5)
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(2)
ELISA (Standard)
(1)
In Vivo
(2)
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Human
(37)
Mouse
(17)
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(6)
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(1)
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(1)
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(1)
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(1)
Primate - Callitrix jacchus (Common Marmoset)
(1)
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(1)
All Species
Showing Publications 1 - 10 of 66. Show All 66 Publications.
Publications using 267-N3/CF Applications Species
S Martin, KC Allan, O Pinkard, T Sweet, PJ Tesar, J Coller Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 2022;13(1):5003. 2022 [PMID: 36008413] (Bioassay, Mouse) Bioassay Mouse
M Sawamura, K Imamura, R Hikawa, T Enami, A Nagahashi, H Yamakado, H Ichijo, T Fujisawa, H Yamashita, S Minamiyama, M Kaido, H Wada, M Urushitani, H Inoue, N Egawa, R Takahashi Cellular analysis of SOD1 protein-aggregation propensity and toxicity: a case of ALS with slow progression harboring homozygous SOD1-D92G mutation Scientific Reports, 2022;12(1):12636. 2022 [PMID: 35879519] (Bioassay, Human) Bioassay Human
MK Eltahir, M Nakamori, S Hattori, T Kimura, H Mochizuki, S Nagano Pharmacotherapy alleviates pathological changes in human direct reprogrammed neuronal cell model of myotonic dystrophy type 1 PLoS ONE, 2022;17(7):e0269683. 2022 [PMID: 35776705] (Bioassay, Human) Bioassay Human
Salokas K, Liu X, Ohman T, Chowdhury I, Gawriyski L, Keskitalo S, Varjosalo M Physical and functional interactome atlas of human receptor tyrosine kinases. EMBO Rep, 0;23(6):e54041. 0 [PMID: 35384245] (Bioassay, Human) Bioassay Human
JM Suárez-Riv, CJ Pastor-Mal, A Romero-Gon, D Gómez-Fern, S Povea-Cabe, M Álvarez-Có, I Villalón-G, M Talaverón-, A Suárez-Car, M Munuera-Ca, JA Sánchez-Al Pterostilbene in Combination With Mitochondrial Cofactors Improve Mitochondrial Function in Cellular Models of Mitochondrial Diseases Frontiers in Pharmacology, 2022;13(0):862085. 2022 [PMID: 35370630] (Bioassay, Human) Bioassay Human
A Bruzelius, S Kidnapilla, J Drouin-Oue, T Stoker, RA Barker, D Rylander O Reprogramming Human Adult Fibroblasts into GABAergic Interneurons Cells, 2021;10(12):. 2021 [PMID: 34943958] (Bioassay, Human) Bioassay Human
D Segelcke, HK Fischer, M Hütte, S Dennerlein, F Benseler, N Brose, EM Pogatzki-Z, M Schmidt Tmem160 contributes to the establishment of discrete nerve injury-induced pain behaviors in male mice Cell Reports, 2021;37(12):110152. 2021 [PMID: 34936870] (Bioassay, Mouse) Bioassay Mouse
P Göttle, K Schichel, L Reiche, L Werner, A Zink, A Prigione, P Küry TLR4 Associated Signaling Disrupters as a New Means to Overcome HERV-W Envelope-Mediated Myelination Deficits Frontiers in Cellular Neuroscience, 2021;15(0):777542. 2021 [PMID: 34887730] (Bioassay, Rat) Bioassay Rat
F Rousset, D Schmidbaue, S Fink, Y Adel, B Obexer, M Müller, R Glueckert, H Löwenheim, P Senn Phoenix auditory neurons as 3R cell model for high throughput screening of neurogenic compounds Hearing research, 2021;414(0):108391. 2021 [PMID: 34844170] (Bioassay, Mouse) Bioassay Mouse
P Chennampal, A Sayed-Zahi, P Soundarara, J Sharp, GA Cox, SD Collins, RL Smith A microfluidic approach to rescue ALS motor neuron degeneration using rapamycin Scientific Reports, 2021;11(1):18168. 2021 [PMID: 34518579] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Show All 66 Publications.

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Bioinformatics

Gene Symbol NTF3
Uniprot