Recombinant Human Netrin-1 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Netrin-1 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Rat UNC5H2 Fc Chimera (Catalog # 1006-UN) is immobilized at 5 μg/mL, Recombinant Human Netrin-1 binds with an apparent Kd <1 nM. Measured in a cell proliferation assay using RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.5-2.0 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Netrin-1 protein
Human Netrin-1
(Val22-Ala604)
Accession # O95631
HPGGGSGGGSGGGS 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Val22
Protein/Peptide Type
Recombinant Proteins
Gene
NTN1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
67.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
80-85 kda, reducing conditions
Publications
Read Publications using
6419-N1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Netrin-1 Protein

  • netrin 1
  • Netrin1
  • Netrin-1
  • NTN1
  • NTN1Lnetrin 1, mouse, homolog of

Background

Human Netrin-1 (netr: Sanskrit for “one who guides”) is a 75 kDa glycoprotein that is closely related to the laminin  gamma  domain and functions as a chemoattractive or chemorepulsive guidance cue in the central nervous system (CNS) during development (1, 2). The protein is synthesized as a 604 amino acid (aa) precursor that contains a 24 aa signal sequence and a 580 aa mature chain. Residues 46-283 constitute a laminin N-terminal domain (domain VI), while 285-453 make up three laminin-type epidermal growth factor‑like domains. There is a final domain that runs from aa 487-601 that qualifies as a Netrin-1 like domain. It is also known as domain C in the context of C. elegans. There are four potential sites for N‑linked glycosylation. Human Netrin-1 is 99% aa identical to mouse and rat Netrin-1. Netrin-1 is expressed in adult and embryonic tissues. In the adult, the protein is expressed in Schwann cells, oligodendrocytes and multiple neurons. In embryonic tissues, Netrin-1 is found in somatic mesoderm, heart, branchial pouch, and neuroepithelium. Netrin-1 is a secreted protein that, in addition to its involvement in outgrowth and migration orientation in the developing CNS, plays a significant role in the morphogenesis of endothelial cells and vascular smooth-muscle cells. It is also involved in the processes of cytoskeleton reorganization, angiogenesis, epithelial cell adhesion, and cell migration in the lungs, mammary gland, and pancreas (1, 3).

Netrin-1 effects are controlled through different transmembrane receptors (2). Four of the receptors exist in the Unc-5 (Unc=uncontrolled behaviorally) family of proteins, and these include Unc5h1, Unc5h2, Unc5h3/RCM, and Unc5h4. There are also two receptors that belong to the UNC-40 family of molecules. The first is DCC (deleted in colorectal cancer), and the second is neogenin (newly‑generated). UNC-5 receptors are noted to mediate the attraction response of axons and netrins. However, UNC-5 molecules, when co‑expressed with DCC in the presence of Netrin-1, will mediate repulsion by varying cellular calcium levels (3). The adenosine A2b receptor may also be involved in chemoattraction, either by binding directly with Netrin-1 or by serving as a co‑receptor for DCC. These receptors are known as dependence receptors because they depend on their ligand, in this case Netrin-1, for survival (4). Unbound, the receptors induce a specific death signal (4). It is the dysregulation of these receptor systems that may have important roles in tumor biology. DCC and UNC5 proteins make up a system for either initiating or inhibiting apoptosis (4), and it is now believed that Netrin-1 and its dependence receptors play a major role in tumor biology (4-6).

  1. Dakouane-Giudicelli, M. et al. (2010) J. Histochem. Cytochem. 58:73.
  2. Masuda, T. et al. (2008) J. of Neurosci. 28:10380.
  3. Bernet, A. and P. Mehlen (2007) Bull. Cancer 94:E12.
  4. Cirulli, V. and M. Yebra, (2007) Nat. Rev. Mol. Cell. Biol. 8:296.
  5. Dumartin, L. et al. (2010) Gastroenterology 137:1595.
  6. Mazelin, L. et al. (2004) Nature 431:80.

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Publications for Netrin-1 (6419-N1)(12)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(9)
In Vivo
(3)
All Applications
Filter By Species
Human
(8)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 6419-N1 Applications Species
B Mondal, H Jin, S Kallappago, Y Sedkov, T Martinez, MF Sentmanat, GJ Poet, C Li, Y Fan, SM Pruett-Mil, HM Herz The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth Elife, 2020;9(0):. 2020 [PMID: 32297854] (Bioassay, Mouse) Bioassay Mouse
CP Prieto, BS Casas, P Falcón, A Villanueva, P Lois, J Lattus, V Palma Downregulation of the Netrin-1 Receptor UNC5b Underlies Increased Placental Angiogenesis in Human Gestational Diabetes Mellitus Int J Mol Sci, 2019;20(6):. 2019 [PMID: 30897795] (Bioassay, Human) Bioassay Human
J Chen, Y Xuan, Y Chen, T Wu, L Chen, H Guan, S Yang, J He, D Shi, Y Wang Netrin-1 alleviates subarachnoid haemorrhage-induced brain injury via the PPAR?/NF-KB signalling pathway J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30614619] (In Vivo, Mouse) In Vivo Mouse
F Huang, M Ben Aissa, G Lévesque, M Carreau FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis BMC Res Notes, 2018;11(1):662. 2018 [PMID: 30213274] (Bioassay, Human) Bioassay Human
F Huang, M Ben Aissa, G Lévesque, M Carreau FANCC localizes with UNC5A at neurite outgrowth and promotes neuritogenesis BMC Res Notes, 2018;11(1):662. 2018 [PMID: 30213274] (Bioassay, Human) Bioassay Human
I Hernandez-, S Koss, S Sharma, MJ Pitman Influence of Netrin-1 on Reinnervation of Laryngeal Muscles Following Recurrent Laryngeal Nerve Injury Neurosci. Lett., 2017;0(0):. 2017 [PMID: 28527715] (In Vivo, Rat) In Vivo Rat
Q Shao, T Yang, H Huang, F Alarmanazi, G Liu Uncoupling of UNC5C with Polymerized TUBB3 in Microtubules Mediates Netrin-1 Repulsion J. Neurosci., 2017;0(0):. 2017 [PMID: 28483977] (Bioassay, Human) Bioassay Human
Z Xie, L Huang, B Enkhjargal, C Reis, W Wan, J Tang, Y Cheng, JH Zhang Intranasal administration of recombinant Netrin-1 attenuates neuronal apoptosis by activating DCC/APPL-1/AKT signaling pathway after subarachnoid hemorrhage in rats Neuropharmacology, 2017;0(0):. 2017 [PMID: 28347836] (In Vivo, Rat) In Vivo Rat
Deming P, Campbell S, Stone J, Rivard R, Mercier A, Howe A Anchoring of protein kinase A by ERM (ezrin-radixin-moesin) proteins is required for proper netrin signaling through DCC (deleted in colorectal cancer). J Biol Chem, 2015;290(9):5783-96. 2015 [PMID: 25575591] (Bioassay, Human) Bioassay Human
Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation. J Bone Miner Res, 2015;30(5):837-54. 2015 [PMID: 25483983] (Bioassay, Human) Bioassay Human
Show All 12 Publications.

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Bioinformatics

Gene Symbol NTN1
Uniprot