Recombinant Human/Mouse/Rat Irisin Protein, CF

3T3-L1 preadipocytes were incubated with 1 µg/ml of RecombinantHuman Irisin for 15 minutes. The cellswere lysed and Western blots were performed with anti-phospho p38 MAPK andtotal p38 MAPK antibodies. Experiments are shown in duplicate.

• Reactivity: Hu, Mu, Rt
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human/Mouse/Rat Irisin Protein, CF Summary

• Details of Functionality: Measured by its ability to induce p38 MAPK activation in 3T3‑L1 mouse embryonic fibroblast adipose-like cells. 1 μg/mL of Recombinant Human Irisin can effectively induce p38 MAPK activation.
• Source: Chinese Hamster Ovary cell line, CHO-derived Irisin/FNDC5 protein
  Asp32-Glu143, with a C-terminal 6-His tag
• Accession #: Q8NAU1
• N-terminal Sequence: Asp32
• Protein/Peptide Type: Recombinant Proteins
• Gene: FNDC5
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 15-37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl and TCEP .
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse/Rat Irisin Protein, CF

• fibronectin type III domain containing 5
• fibronectin type III domain-containing protein 5
• FNDC5
• FRCP2
• FRCP2Fibronectin type III repeat-containing protein 2
• Irisin

Background

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

1. Bostrom, P. et al. (2012) Nature 481:463.
2. Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
3. Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
4. Teufel, A. et al. (2002) Gene 297:79.
5. Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
6. Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
7. Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
8. Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
9. Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
10. Dun, S.L. et al. (2013) Neuroscience 240:155.
11. Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
12. Hashemi, M.S. et al. (2013) Neuroscience 231:296.
13. Moon, H.S. et al. (2013) Metabolism 62:1131.
14. Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.

Publications for Irisin/FNDC5 (8880-IR/CF)(1)

Publication using 8880-IR/CF

• W Zhou, Y Shi, H Wang, L Chen, C Yu, X Zhang, L Yang, X Zhang, A Wu Exercise-induced FNDC5/irisin protects nucleus pulposus cells against senescence and apoptosis by activating autophagy Oncogene, 2022-07-26;54(7):1038-1048. 2022-07-26 [PMID: 35882943] (Bioassay, Rat)

Bioinformatics

• Gene Symbol: FNDC5
• Uniprot:
  • Q8NAU1()