Recombinant Human/Mouse FGF-8b Protein, CF


1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications BA

Order Details

Recombinant Human/Mouse FGF-8b Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin.
E. coli-derived
Gln23-Arg215, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
23 kDa, reducing condtions
Read Publications using
423-F8/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and Brij-35.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse FGF-8b Protein, CF

  • AIGF
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376


FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

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Publications for (33)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Rabbit, Xenopus, Zebrafish.

We have publications tested in 4 applications: Bioassay, Expansion/Differentiation, In Ovo, In Vivo.

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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using 423-F8/CF Applications Species
AR Rodrigues, N Yakushiji-, Y Atsuta, G Andrey, P Schorderet, D Duboule, CJ Tabin Integration of Shh and Fgf signaling in controlling Hox gene expression in cultured limb cells Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017 [PMID: 28270602] (Bioassay, Chicken) Bioassay Chicken
SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres Neurotoxic reactive astrocytes are induced by activated microglia Nature, 2017;541(7638):481-487. 2017 [PMID: 28099414] (Bioassay, Human) Bioassay Human
Fibulin-1 Binds to Fibroblast Growth Factor 8 with High Affinity: Effects on Embryo Survival J Biol Chem, 2016;0(0):. 2016 [PMID: 27402846] (Bioassay, Human) Bioassay Human
Daniel A Monti N-Acetyl Cysteine May Support Dopamine Neurons in Parkinson&#039;s Disease: Preliminary Clinical and Cell Line Data PLoS ONE, 2016;11(6):e0157602. 2016 [PMID: 27309537] (Bioassay, Human) Bioassay Human
Eva Hedlund Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors Sci Rep, 2016;6(0):26448. 2016 [PMID: 27246266] (Bioassay, Mouse) Bioassay Mouse
Chan W, Howe K, Clegg J, Guimond S, Price D, Turnbull J, Pratt T 2-O Heparan Sulfate Sulfation by Hs2st Is Required for Erk/Mapk Signalling Activation at the Mid-Gestational Mouse Telencephalic Midline. PLoS ONE, 2015;10(6):e0130147. 2015 [PMID: 26075383] (Bioassay, Mouse) Bioassay Mouse
Shen, Shih-Che, Shen, Ching-I, Lin, Ho, Chen, Chun-Jun, Chang, Chia-Yu, Chen, Sheng-Me, Lee, Hsiu-Chi, Lai, Ping-Sha, Su, Hong-Lin Susceptibility of human embryonic stem cell-derived neural cells to Japanese encephalitis virus infection. PLoS ONE, 2014;9(12):e114990. 2014 [PMID: 25517725] (Bioassay, Human) Bioassay Human
Attia L, Schneider J, Yelin R, Schultheiss T Collective cell migration of the nephric duct requires FGF signaling. Dev Dyn, 2015;244(2):157-67. 2015 [PMID: 25516335] (Bioassay, Chicken) Bioassay Chicken
Yamamoto-Shiraishi Y, Higuchi H, Yamamoto S, Hirano M, Kuroiwa A Etv1 and Ewsr1 cooperatively regulate limb mesenchymal Fgf10 expression in response to apical ectodermal ridge-derived fibroblast growth factor signal. Dev Biol, 2014;394(1):181-90. 2014 [PMID: 25109552] (Bioassay, Chicken) Bioassay Chicken
Lewandowski J, Pursell T, Rabinowitz A, Vokes S Manipulating gene expression and signaling activity in cultured mouse limb bud cells. Dev Dyn, 2014;243(7):928-36. 2014 [PMID: 24633820] (Bioassay, Mouse) Bioassay Mouse
Show All 33 Publications.

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Gene Symbol FGF8