Recombinant Human/Mouse FGF-8b Protein, CF

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1 μg/lane of Recombinant Human/Mouse FGF-8b was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 23 kDa.
Recombinant Human/Mouse FGF-8b (Catalog # 423-F8/CF) stimulates cell proliferation in the NR6R‑3T3 mouse fibroblast cell line. The ED50 for this effect is 6.5-40 ng/mL in the presence of 1 μg/mL heparin.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Catalog# & Formulation Size Price

Recombinant Human/Mouse FGF-8b Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 6.5-40 ng/mL in the presence of 1 µg/mL heparin.
Source
E. coli-derived FGF-8 protein
Gln23-Arg215, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
FGF8
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23 kDa, reducing condtions
Publications
Read Publications using
423-F8/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4 and Brij-35.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human/Mouse FGF-8b Protein, CF

  • AIGF
  • AIGFKAL6
  • Androgen-induced growth factor
  • FGF8
  • FGF-8
  • fibroblast growth factor 8 (androgen-induced)
  • fibroblast growth factor 8
  • HBGF-8
  • Heparin-binding growth factor 8
  • MGC149376

Background

FGF-8 is a member of the fibroblast growth factor family that was originally discovered as a growth factor essential for the androgen-dependent growth of mouse mammary carcinoma cells (1-3). Alternate splicing of mouse FGF-8 mRNA generates eight secreted isoforms, designated a-h, but only FGF-8a, b, e and f exist in humans (4). FGF-8 contains a 22 amino acid (aa) signal sequence, an N‑terminal domain that varies according to the isoform (30 aa for FGF-8b; 20 aa for the shortest, FGF-8a), a 125 aa FGF domain and a 37 aa proline‑rich C‑terminal sequence. The FGF domain of FGF-8 shares the most aa identity with FGF17 (75%) and FGF-18 (67%), and the three form an FGF subfamily (2). Mouse FGF-8b shares 100% aa identity with human FGF-8b. FGF-8 is widely expressed during embryogenesis, and mediates epithelial-mesenchymal transitions. It plays an organizing and inducing role during gastrulation, and regulates patterning of the midbrain/hindbrain, eye, ear, limbs and heart in the embryo (2, 5 - 8). The isoforms may play different roles in development. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity although FGF-8a and FGF-8e are also transforming and have been found in human prostate, breast or ovarian tumors (1, 5, 9-12). FGF-8 shows limited expression in the normal adult, but low levels are found in the reproductive and genitourinary tract, peripheral leukocytes and bone marrow hematopoietic cells (3, 9, 13).

  1. Mattila, M.M. and P.L. Harkonen (2007) Cytokine Growth Factor Rev. 18:257.
  2. Reuss, B. and O. von Bohlen und Halbach (2003) Cell Tiss. Res. 313:139.
  3. Tanaka, A. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8928.
  4. Gemel, J. et al. (1996) Genomics 35:253.
  5. Olsen, S.K. et al. (2006) Genes Dev. 20:185.
  6. Crossley, P.H. et al. (1996) Cell, 84:127.
  7. Heikinheimo, M. et al. (1994) Mech. Dev. 48:129.
  8. Sun, X. et al. (1999) Genes Dev. 13:1834.
  9. Ghosh, A.K. et al. (1996) Cell Growth Differ. 7:1425.
  10. Mattila, M.M. et al. (2001) Oncogene 20:2791.
  11. Valve, E. et al. (2000) Int. J. Cancer 88:718.
  12. Valve, E.M. et al. (2001) Lab. Invest. 81:815.
  13. Nezu, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:843.

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423-F8/CF
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Applications: Bioactivity

Publications for FGF-8 (423-F8/CF)(46)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Avian - Quail, Chicken, Rabbit, Xenopus, Zebrafish.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, In Ovo, In Vivo.


Filter By Application
Bioassay
(32)
Cell Culture
(3)
Differentiation
(2)
In Ovo
(1)
In Vivo
(8)
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Human
(18)
Mouse
(10)
Rat
(3)
Avian - Quail
(1)
Chicken
(8)
Rabbit
(1)
Xenopus
(2)
Zebrafish
(3)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using 423-F8/CF Applications Species
KX Liang, CK Kristianse, S Mostafavi, GH Vatne, GA Zantingh, A Kianian, C Tzoulis, LE Høyland, M Ziegler, RM Perez, J Furriol, Z Zhang, N Balafkan, Y Hong, R Siller, GJ Sullivan, LA Bindoff Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations EMBO Mol Med, 2020;0(0):e12146. 2020 [PMID: 32840960] (Bioassay, Human) Bioassay Human
H Gla beta, P Neumann, A Pal, P Reinhardt, A Storch, J Sternecker, A Hermann Combined Dendritic and Axonal Deterioration Are Responsible for Motoneuronopathy in Patient-Derived Neuronal Cell Models of Chorea-Acanthocytosis Int J Mol Sci, 2020;21(5):. 2020 [PMID: 32151030] (Cell Culture, Human) Cell Culture Human
S Mahajani, A Raina, C Fokken, S Kügler, M Bähr Homogenous generation of dopaminergic neurons from multiple hiPSC lines by transient expression of transcription factors Cell Death Dis, 2019;10(12):898. 2019 [PMID: 31776327] (Cell Culture, Human) Cell Culture Human
I Allodi, J Nijssen, JA Benitez, C Schweingru, A Fuchs, G Bonvicini, M Cao, O Kiehn, E Hedlund Modeling Motor Neuron Resilience in ALS Using Stem Cells Stem Cell Reports, 2019;0(0):. 2019 [PMID: 31080111] (Bioassay, Mouse) Bioassay Mouse
M Elkouris, G Kouroupi, A Vourvoukel, N Papagianna, V Kaltezioti, R Matsas, L Stefanis, M Xilouri, PK Politis Long Non-coding RNAs Associated With Neurodegeneration-Linked Genes Are Reduced in Parkinson&#039;s Disease Patients Front Cell Neurosci, 2019;13(0):58. 2019 [PMID: 30853899] (Bioassay, Human) Bioassay Human
G Collo, L Cavalleri, M Zoli, U Maskos, E Ratti, E Merlo Pich Alpha6-Containing Nicotinic Acetylcholine Receptors Mediate Nicotine-Induced Structural Plasticity in Mouse and Human iPSC-Derived Dopaminergic Neurons Front Pharmacol, 2018;9(0):572. 2018 [PMID: 29910731] (Bioassay, Human) Bioassay Human
J Li, F Xing, F Chen, L He, KF So, Y Liu, J Xiao Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018;0(0):9636897187803. 2018 [PMID: 29895168] (Bioassay, Human) Bioassay Human
X Hu, JW Lee, X Zheng, J Zhang, X Lin, Y Song, B Wang, X Hu, HH Chang, Y Chen, CP Lin, Y Zhang Efficient induction of functional ameloblasts from human keratinocyte stem cells Stem Cell Res Ther, 2018;9(1):126. 2018 [PMID: 29720250] (Bioassay, Human) Bioassay Human
G Collo, L Cavalleri, F Bono, C Mora, S Fedele, RW Invernizzi, M Gennarelli, G Piovani, T Kunath, MJ Millan, E Merlo Pich, P Spano Ropinirole and Pramipexole Promote Structural Plasticity in Human iPSC-Derived Dopaminergic Neurons via BDNF and mTOR Signaling Neural Plast., 2018;2018(0):4196961. 2018 [PMID: 29531524] (Differentiation, Human) Differentiation Human
G Shall, M Menosky, S Decker, P Nethala, R Welchko, X Leveque, M Lu, M Sandstrom, U Hochgeschw, J Rossignol, G Dunbar Effects of Passage Number and Differentiation Protocol on the Generation of Dopaminergic Neurons from Rat Bone Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2018;19(3):. 2018 [PMID: 29498713] (Bioassay, Rat) Bioassay Rat
Show All 46 Publications.

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