Recombinant Human MMR/CD206 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity

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Recombinant Human MMR/CD206 Protein Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Mannan is coated at 0.2 μg/mL (100 μL/well), the concentration of Recombinant Human MMR/CD206 that produces 50% of the optimal binding response is approximately 0.2-1.2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human MMR/CD206 protein
Leu19-Lys1383 (Thr399Ala) & (Leu407Phe), with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu19
Protein/Peptide Type
Recombinant Proteins
Gene
MRC1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
157 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
180 kDa, reducing conditions
Publications
Read Publications using
2534-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MMR/CD206 Protein

  • CD206
  • CLEC13D
  • CLEC13Dmacrophage mannose receptor 1
  • C-type lectin domain family 13 member D
  • mannose receptor, C type 1
  • MMR
  • MMRCD206 antigen
  • MRC1

Background

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175‑190 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) human CD206 precursor contains a signal sequence (18 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1371 aa), a transmembrane segment and a short (46 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The human CD206 ECD shares 83%, 84%, 89%, 89% and 90% aa sequence identity with mouse, rat, equine, porcine and canine CD206, respectively. The cysteine‑rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewisa and ‑Lewisx (1, 7, 9). The FNII domain mediates Ca2+‑independent binding of collagens (2, 10). The CTLDs participate in Ca2+‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin‑coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

  1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
  2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
  3. Harris, N. et al. (1992) Blood 80:2363.
  4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
  5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
  6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
  8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
  9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
  11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
  12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

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Publications for MMR/CD206/Mannose Receptor (2534-MR)(5)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Bioassay, Surface Plasmon Resonance.


Filter By Application
Bioassay
(3)
Surface Plasmon Resonance
(2)
All Applications
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Human
(4)
All Species
Showing Publications 1 - 5 of 5.
Publications using 2534-MR Applications Species
R Yang, M Wu, S Lin, RP Nargund, X Li, T Kelly, L Yan, G Dai, Y Qian, Q Dallas-Yan, PA Fischer, Y Cui, X Shen, P Huo, DD Feng, MD Erion, DE Kelley, J Mu A glucose-responsive insulin therapy protects animals against hypoglycemia JCI Insight, 2018;3(1):. 2018 [PMID: 29321379] (Bioassay) Bioassay
Chung E, Lee G, Lee C, Ye M, Chung H, Kim H, Bae S, Hwang D, Bae H Bee Venom Phospholipase A2, a Novel Foxp3+ Regulatory T Cell Inducer, Protects Dopaminergic Neurons by Modulating Neuroinflammatory Responses in a Mouse Model of Parkinson&#039;s Disease. J Immunol, 2015;195(10):4853-60. 2015 [PMID: 26453752] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015;10(3):e0119244. 2015 [PMID: 25786219] (Bioassay, Human) Bioassay Human
Nandakumar, Subhadra, Kannanganat, Sunil, Dobos, Karen M, Lucas, Megan, Spencer, John S, Fang, Sunan, McDonald, Melissa, Pohl, Jan, Birkness, Kristin, Chamcha, Venkates, Ramirez, Melissa, Plikaytis, Bonnie B, Posey, James E, Amara, Rama Rao, Sable, Suraj B O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection. PLoS Pathog, 2013;9(10):e1003705. 2013 [PMID: 24130497] (Bioassay, Human) Bioassay Human
Gustafsson A, Sjoblom M, Strindelius L, Johansson T, Fleckenstein T, Chatzissavidou N, Lindberg L, Angstrom J, Rova U, Holgersson J Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system. Glycobiology, 2011;21(8):1071-86. 2011 [PMID: 21474492] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human

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Bioinformatics

Gene Symbol MRC1
Entrez
Uniprot