Recombinant Human MMR/CD206 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human MMR/CD206 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Mannan is coated at 0.2 μg/mL (100 μL/well), the concentration of Recombinant Human MMR/CD206 that produces 50% of the optimal binding response is approximately 0.200-1.20 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human MMR/CD206 protein
  Leu19-Lys1383 (Thr399Ala) & (Leu407Phe), with a C-terminal 6-His tag
• Accession #: P22897
• N-terminal Sequence: Leu19
• Protein/Peptide Type: Recombinant Proteins
• Gene: MRC1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 157 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 160 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MMR/CD206 Protein, CF

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C‑type lectin domain family 13, member D) (1‑4). CD206 is a 175‑190 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor‑associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1456 amino acid (aa) human CD206 precursor contains a signal sequence (18 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II repeat, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1371 aa), a transmembrane segment and a short (46 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The human CD206 ECD shares 83%, 84%, 89%, 89% and 90% aa sequence identity with mouse, rat, equine, porcine and canine CD206, respectively. The cysteine‑rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewis^a and ‑Lewis^x (1, 7, 9). The FNII domain mediates Ca2⁺‑independent binding of collagens (2, 10). The CTLDs participate in Ca2⁺‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin‑coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
3. Harris, N. et al. (1992) Blood 80:2363.
4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

Publications for MMR/CD206/Mannose Receptor (2534-MR/CF)(11)

Publications using 2534-MR/CF

• M Bruno, S Kersten, JM Bain, M Jaeger, D Rosati, MD Kruppa, DW Lowman, PJ Rice, B Graves, Z Ma, YN Jiao, A Chowdhary, G Renieris, FL van de Vee, BJ Kullberg, EJ Giamarello, A Hoischen, NAR Gow, AJP Brown, JF Meis, DL Williams, MG Netea Transcriptional and functional insights into the host immune response against the emerging fungal pathogen Candida auris Nat Microbiol, 2020-08-24;0(0):. 2020-08-24 [PMID: 32839538] (Bioassay, Human)
• PF Duffney, HH Kim, NA Porter, I Jaspers Ozone-derived oxysterols impair lung macrophage phagocytosis via adduction of some phagocytosis receptors J. Biol. Chem., 2020-07-20;0(0):. 2020-07-20 [PMID: 32690608] (ELISA Development, Human)
• I Kucinskait, M Simanavici, J Dapkunas, M Pleckaityt, A Zvirbliene Mapping of Recognition Sites of Monoclonal Antibodies Responsible for the Inhibition of Pneumolysin Functional Activity Biomolecules, 2020-07-08;10(7):. 2020-07-08 [PMID: 32650398] (ELISA (detection), Human)
• J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019-07-25;14(7):e0219480. 2019-07-25 [PMID: 31344060] (Bioassay, Human)
• E Bartheldyo, P Turánek Kn, K Zachová, J Mašek, P Kulich, R Effenberg, D Zyka, F Hubatka, J Kotou?ek, H ?elechovsk, R Héžová, A Tome?ková, E Mašková, M Fojtíková, S Macaulay, P Bystrický, L Paulovi?ov, E Paulovi?ov, L Drož, M Ledvina, M Raška, J Turánek N-Oxy lipid-based click chemistry for orthogonal coupling of mannan onto nanoliposomes prepared by microfluidic mixing: Synthesis of lipids, characterisation of mannan-coated nanoliposomes and in vitro stimulation of dendritic cells Carbohydr Polym, 2018-11-29;207(0):521-532. 2018-11-29 [PMID: 30600036] (Binding Assay)
• K Subramania, DR Neill, HA Malak, L Spelmink, S Khandaker, G Dalla Libe, E Dearing, A Kirby, M Yang, A Achour, J Nilvebrant, PÅ Nygren, L Plant, A Kadioglu, B Henriques- Pneumolysin binds to the mannose receptor C type 1 (MRC-1) leading to anti-inflammatory responses and enhanced pneumococcal survival Nat Microbiol, 2018-11-12;0(0):. 2018-11-12 [PMID: 30420782] (Surface Plasmon Resonance)
• R Yang, M Wu, S Lin, RP Nargund, X Li, T Kelly, L Yan, G Dai, Y Qian, Q Dallas-Yan, PA Fischer, Y Cui, X Shen, P Huo, DD Feng, MD Erion, DE Kelley, J Mu A glucose-responsive insulin therapy protects animals against hypoglycemia JCI Insight, 2018-01-11;3(1):. 2018-01-11 [PMID: 29321379] (Bioassay)
• Chung E, Lee G, Lee C, Ye M, Chung H, Kim H, Bae S, Hwang D, Bae H Bee Venom Phospholipase A2, a Novel Foxp3+ Regulatory T Cell Inducer, Protects Dopaminergic Neurons by Modulating Neuroinflammatory Responses in a Mouse Model of Parkinson&#039;s Disease. J Immunol, 2015-10-09;195(10):4853-60. 2015-10-09 [PMID: 26453752] (Surface Plasmon Resonance, Human)
• Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015-03-18;10(3):e0119244. 2015-03-18 [PMID: 25786219] (Bioassay, Human)
• Nandakumar , Subhadra, Kannanganat , Sunil, Dobos , Karen M, Lucas , Megan, Spencer , John S, Fang , Sunan, McDonald , Melissa, Pohl , Jan, Birkness , Kristin, Chamcha , Venkates, Ramirez , Melissa, Plikaytis , Bonnie B, Posey , James E, Amara , Rama Rao, Sable , Suraj B O-mannosylation of the Mycobacterium tuberculosis adhesin Apa is crucial for T cell antigenicity during infection but is expendable for protection. PLoS Pathog, 2013-10-10;9(10):e1003705. 2013-10-10 [PMID: 24130497] (Bioassay, Human)
• Gustafsson A, Sjoblom M, Strindelius L, Johansson T, Fleckenstein T, Chatzissavidou N, Lindberg L, Angstrom J, Rova U, Holgersson J Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system. Glycobiology, 2011-04-07;21(8):1071-86. 2011-04-07 [PMID: 21474492] (Surface Plasmon Resonance, Human)

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • P22897()