Recombinant Human mGluR4 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human mGluR4 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human mGluR4 protein
  Lys33-Ser518, with a C-terminal 6-His tag
• Accession #: Q14833-1
• N-terminal Sequence: Lys33
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 62-76 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human mGluR4 Protein, CF

• glutamate receptor, metabotropic 4
• GPRC1D
• GPRC1Dmetabotropic glutamate receptor 4
• GRM4
• MGC177594
• mGlu4
• mGluR4
• MGLUR4glutamate metabotropic receptor 4

Background

Metabotropic glutamate receptors (mGluRs) are members of the G-protein-coupled receptor (GPCR) superfamily, modulating glutamate neurotransmission in the central and peripheral nervous systems through GTP-binding proteins (1). Structurally, members of this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane domain, and a cytoplasmic C-terminal domain that is variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (2). The C-terminal region is subject to alternative splicing, regulation by phosphorylation and interacts directly with a G-protein to modulate protein-protein interactions (2, 3). The receptors are subdivided into three groups (I–III) based on sequence homology, signal transduction and pharmacological properties (1, 2). The Group III receptors include mGLuRs 4, 6, 7 and 8 (2). mGLuR4 has been shown to be expressed postsynaptically in certain areas such as the hippocampus and retina (4). Mature human mGluR4 is 880 amino acids in length. In the ECD region comprising amino acids (aa) 33-587, human mGluR4 shares 97% aa sequence identity with mouse and rat mGluR4.

1. Conn P.J. et al. (1997) Annu. Rev. Pharmacol. Toxicol. 37:205.
2. Niswender C.M. et al. (2010) Annu. Rev. Pharmacol. Toxicol. 5:295.
3. Pin J.P. et al. (1995) Neuropharmacology. 34:1.
4. Bradley, S.R. et al. (1996) J. Neurosci. 16:2044.

Bioinformatics

• Uniprot:
  • Q14833-1()