Recombinant Human mGluR3 Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human mGluR3 Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Chinese Hamster Ovary cell line, CHO-derived human mGluR3 protein
  Asp25-Ser507, with a C-terminal 6-His tag
• Accession #: Q14832-1
• N-terminal Sequence: Asp25
• Structure / Form: Disulfide linked homodimer
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-74 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human mGluR3 Protein, CF

• GLUR3
• glutamate receptor, metabotropic 3
• GPRC1C
• GPRC1Cglutamate metabotropic receptor 3
• GRM3
• mGlu3
• mGluR3
• MGLUR3metabotropic glutamate receptor 3

Background

Metabotropic glutamate receptors (mGluRs) are coupled to effector systems through GTP-binding proteins and modulate glutamate neurotransmission in the central and peripheral nervous systems (1). Structurally, members from this family are characterized by a large N-terminal extracellular domain (ECD), seven transmembrane, and a cytoplasmic carboxyl-terminal domain variable in length. Two ECDs dimerize together and large conformational changes are induced when agonists bind to one or both domains (3). Intracellularly, a short C-terminus interacts directly with a G-protein (2). The receptors are subdivided into three groups (I-III) based on sequence homology, signal transduction and pharmacological properties (1). The Group II receptors comprise mGluR2 and mGluR3, which share high sequence homology. mGluR3 is a presynaptic receptor expressed on both neurons and glia, and its activation regulates cAMP accumulation (1). Mature human mGluR3 is 857 amino acids (aa) in length and aa 2-507 represents the N-terminal ECD (4), with the apparent molecular mass approximately 66 kDa in SDS-PAGE under reducing conditions. Over aa 2-507, human mGluR3 shares 97% aa sequence identity with mouse and rat mGluR3.

1. Conn PJ, et al. (1997) Annu Rev Pharmacol Toxicol 37:205.
2. Pin JP, et al. (1995) Neuropharmacology. 34:1.
3. Niswender CM, et al. (2010) Annu Rev Pharmacol Toxicol 50:295.
4. Monn, J.A. et al. (2015) J Med Chem 58:7526.

Bioinformatics

• Uniprot:
  • Q14832-1()