Recombinant Human MFG-E8 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human MFG-E8 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. When 5 x 104 cells/well are added to Recombinant Human MFG‑E8 coated plates (10 µg/mL, 100 µL/well), approximately 45‑75% will adhere after 60 minutes at 37 °C.
Source
Mouse myeloma cell line, NS0-derived human MFG-E8 protein
Leu24-Cys387, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu24
Protein/Peptide Type
Recombinant Proteins
Gene
MFGE8
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-58 kDa, reducing conditions
Publications
Read Publications using
2767-MF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4 and NaCl.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MFG-E8 Protein, CF

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66-75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Couto, J.R. et al. (1996) DNA Cell Biol. 15:281.
  3. Yamaguchi, H. et al. (2010) Eur. J. Immunol. 40:1778.
  4. Haggqvist, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:8669.
  5. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  6. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  7. Hanayama, R. et al. (2002) Nature 417:182.
  8. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  9. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  10. Hanayama, R. et al. (2004) Science 304:1147.
  11. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  12. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  13. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  14. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  15. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

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Publications for MFG-E8 (2767-MF)(14)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Bioassay, Cell Culture, ELISA (Standard), In Vivo, Surface Plasmon Resonance.


Filter By Application
Bioassay
(10)
Cell Culture
(1)
ELISA (Standard)
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(11)
Mouse
(1)
Rat
(1)
N/A
(1)
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Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 2767-MF Applications Species
Z Pan, EK Dumas, C Lawrence, L Pate, S Longobardi, X Wang, JA James, S Kovats, AD Farris Bacillus anthracis Edema Toxin Inhibits Efferocytosis in Human Macrophages and Alters Efferocytic Receptor Signaling Int J Mol Sci, 2019;20(5):. 2019 [PMID: 30866434] (Bioassay, Human) Bioassay Human
YY Gao, ZH Zhang, Z Zhuang, Y Lu, LY Wu, ZN Ye, XS Zhang, CL Chen, W Li, CH Hang Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin ?3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury Cell Death Dis, 2018;9(9):845. 2018 [PMID: 30154436] (In Vivo, Rat) In Vivo Rat
MA Calton, MO Beaulieu, G Benchorin, D Vollrath Method for measuring extracellular flux from intact polarized epithelial monolayers Mol. Vis., 2018;24(0):425-433. 2018 [PMID: 30034209] (Bioassay, Human) Bioassay Human
Y Nakashima, C Miyagi-Shi, H Noguchi, T Omasa Atorvastatin Inhibits the HIF1?-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells Mol. Ther., 2018;0(0):. 2018 [PMID: 29929789] (Bioassay, Human) Bioassay Human
G Inana, C Murat, W An, X Yao, IR Harris, J Cao RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018;16(1):63. 2018 [PMID: 29534722] (Bioassay, Human) Bioassay Human
C Müller, C Charniga, S Temple, SC Finnemann Quantified F-Actin Morphology Is Predictive of Phagocytic Capacity of Stem Cell-Derived Retinal Pigment Epithelium Stem Cell Reports, 2018;0(0):. 2018 [PMID: 29456184] (Bioassay, Human) Bioassay Human
Q Zhao, L Xu, X Sun, K Zhang, H Shen, Y Tian, F Sun, Y Li MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs signalling Tumour Biol., 2017;39(6):1010428317707. 2017 [PMID: 28653875] (Bioassay, Human) Bioassay Human
A novel affinity-based method for the isolation of highly purified extracellular vesicles Sci Rep, 2016;6(0):33935. 2016 [PMID: 27659060] (Bioassay, Human) Bioassay Human
Heo K, Cushman H, Akaike M, Woo C, Wang X, Qiu X, Fujiwara K, Abe J ERK5 activation in macrophages promotes efferocytosis and inhibits atherosclerosis. Circulation, 2014;130(2):180-91. 2014 [PMID: 25001623] (Bioassay, Mouse) Bioassay Mouse
Abe T, Shin J, Hosur K, Udey M, Chavakis T, Hajishengallis G Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol, 2014;193(3):1383-91. 2014 [PMID: 24958900] (Bioassay, Human) Bioassay Human
Show All 14 Publications.

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Bioinformatics

Gene Symbol MFGE8
Uniprot