Recombinant Human M-CSF R/CD115 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human M-CSF R/CD115 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit the M-CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Halenbeck, R. et al. (1989) Biotechnology 7:710. The ED₅₀ for this effect is 0.004-0.012 µg/mL in the presence of 1 ng/mL of Recombinant Human M-CSF (Catalog # 216-MC).
• Source: Mouse myeloma cell line, NS0-derived human M-CSF R/CD115 protein
  

  Human M-CSF R (Ile20-Glu512) Accession # CAA27300	DIEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: CAA27300
• N-terminal Sequence: Ile20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: CSF1R
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 81 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 109-128 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human M-CSF R/CD115 Fc Chimera Protein, CF

• CD115 antigen
• CD115
• c-fms
• colony stimulating factor 1 receptor
• CSF1R
• CSF-1-R
• CSFR
• EC 2.7.10.1
• FMS proto-oncogene
• FMSFIM2
• macrophage colony stimulating factor I receptor
• macrophage colony-stimulating factor 1 receptor
• McDonough feline sarcoma viral (v-fms) oncogene homolog
• M-CSF R
• MCSFR
• M-CSFR
• Proto-oncogene c-Fms

Background

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75% and 76% aa identity with mouse, rat, bovine, canine, feline and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin  alpha _v beta ₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased
M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
7. Dai, X. et al. (2002) Blood 99:111.
8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
9. Li, M. et al. (2004) J. Neurochem. 91:623.
10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

Publications for M-CSFR/CD115 (329-MR/CF)(6)

Publications using 329-MR/CF

• Effects of Cigarette Smoke Condensate on Oxidative Stress, Apoptotic Cell Death, and HIV Replication in Human Monocytic Cells PLoS ONE, 2016-05-20;11(5):e0155791. 2016-05-20 [PMID: 27203850] (Bioassay, Human)
• Enhanced antagonism of BST-2 by a neurovirulent SIV envelope J Clin Invest, 2016-05-09;0(0):. 2016-05-09 [PMID: 27159392] (Bioassay, Primate)
• The nuclear receptor LXR modulates interleukin-18 levels in macrophages through multiple mechanisms Sci Rep, 2016-05-06;6(0):25481. 2016-05-06 [PMID: 27149934] (Bioassay, Human)
• JE Ussher, B van Wilgen, RF Hannaway, K Ruustal, P Phalora, A Kurioka, TH Hansen, CB Willberg, RE Phillips, P Klenerman TLR signalling in human antigen-presenting cells regulates MR1-dependent activation of MAIT cells Eur J Immunol, 2016-05-30;0(0):. 2016-05-30 [PMID: 27105778] (Bioassay, Human)
• AS Haka, VC Barbosa-Lo, HJ Lee, DJ Falcone, CA Hudis, AJ Dannenberg, FR Maxfield Exocytosis of Macrophage Lysosomes Leads to Digestion of Apoptotic Adipocytes and Foam Cell Formation J Lipid Res, 2016-04-04;0(0):. 2016-04-04 [PMID: 27044658] (Bioassay, Human)
• Lamprecht B, Walter K, Kreher S, Kumar R, Hummel M, Lenze D, Kochert K, Bouhlel MA, Richter J, Soler E, Stadhouders R, Johrens K, Wurster KD, Callen DF, Harte MF, Giefing M, Barlow R, Stein H, Anagnostopoulos I, Janz M, Cockerill PN, Siebert R, Dorken B, Bonifer C, Mathas S Derepression of an endogenous long terminal repeat activates the CSF1R proto-oncogene in human lymphoma. Nat. Med., 2010-05-02;16(5):571-9. 2010-05-02 [PMID: 20436485] (Bioassay, Human)

Bioinformatics

• Gene Symbol: CSF1R
• Uniprot:
  • CAA27300()