Recombinant Human LAP (TGF-beta 1) Protein


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Applications Bioactivity

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Recombinant Human LAP (TGF-beta 1) Protein Summary

Details of Functionality
Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 50-300 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF‑ beta 1 (Catalog # 240-B).
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human LAP (TGF-beta 1) protein
Leu30-Arg278 (Cys33Ser)
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
27 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
28-36 kDa, reducing & 60-70 kDa, non-reducing conditions (variably glycosylated)
Read Publications using
246-LP in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LAP (TGF-beta 1) Protein

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1


TGF-  beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF‑  beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 1 (4‑6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (4‑8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).

  1. Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  2. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  3. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  4. Derynck, R. et al. (1985) Nature 316:701.
  5. Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179.
  6. Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660.
  7. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  8. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  9. Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694.
  10. Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.

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Publications for LAP (TGF-beta 1) (246-LP)(22)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Porcine.

We have publications tested in 4 applications: Antibody Production, Bioassay, In Vivo, Surface Plasmon Resonance.

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In Vivo
Surface Plasmon Resonance
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Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 246-LP Applications Species
M Zhou, J Niu, J Wang, H Gao, M Shahbaz, Z Niu, Z Li, X Zou, B Liang Integrin &amp;alphav&amp;beta8 serves as a Novel Marker of Poor Prognosis in Colon Carcinoma and Regulates Cell Invasiveness through the Activation of TGF-&amp;beta1 J Cancer, 2020;11(13):3803-3815. 2020 [PMID: 32328185] (Bioassay, Human) Bioassay Human
J Basta, L Robbins, L Stout, MJ Prinsen, DW Griggs, M Rauchman Pharmacologic inhibition of RGD-binding integrins ameliorates fibrosis and improves function following kidney injury Physiol Rep, 2020;8(7):e14329. 2020 [PMID: 32281744] (Bioassay, Human) Bioassay Human
DC Briggs, AWW Langford-S, HL Birchenoug, TA Jowitt, CM Kielty, JJ Enghild, C Baldock, CM Milner, AJ Day Inter-&amp;alpha-inhibitor heavy chain-1 has an integrin-like 3D structure mediating immune regulatory activities and matrix stabilization during ovulation J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32144206] (Surface Plasmon Resonance) Surface Plasmon Resonance
Lynne A Murray The TGF-? inhibitory activity of antibody 37E1B5 depends on its H-CDR2 glycan MAbs, 2016;0(0):0. 2016 [PMID: 27834568] (Bioassay, Mouse) Bioassay Mouse
PSG9 Stimulates Increase in FoxP3+ Regulatory T-Cells through the TGF-?1 Pathway PLoS ONE, 2016;11(7):e0158050. 2016 [PMID: 27389696] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice J. Biol. Chem., 2016;291(15):8070-89. 2016 [PMID: 26663085] (Bioassay, N/A) Bioassay N/A
Turner C, Badu-Nkansah K, Crowley D, van der Flier A, Hynes R alpha5 and alphav integrins cooperate to regulate vascular smooth muscle and neural crest functions in vivo. Development, 2015;142(4):797-808. 2015 [PMID: 25670798] (Bioassay, Mouse) Bioassay Mouse
Fedele C, Singh A, Zerlanko B, Iozzo R, Languino L The alphavbeta6 integrin is transferred intercellularly via exosomes. J Biol Chem, 2015;290(8):4545-51. 2015 [PMID: 25568317] (Bioassay, Human) Bioassay Human
Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). J Biol Chem, 2015;290(7):4422-31. 2015 [PMID: 25548275] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response. PLoS Negl Trop Dis, 2014;8(7):e2995. 2014 [PMID: 25032977] (Bioassay, Human) Bioassay Human
Show All 22 Publications.

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Gene Symbol TGFB1