Recombinant Human LAP (TGF-beta 1) Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human LAP (TGF-beta 1) Protein Summary

Details of Functionality
Measured by its ability to inhibit TGF-beta 1 activity on HT‑2 mouse T cells. Tsang, M. et al. (1995) Cytokine 7:389. The ED50 for this effect is 50-300 ng/mL in the presence of 1 ng/mL of Recombinant Human TGF‑ beta 1 (Catalog # 240-B).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human LAP (TGF-beta 1) protein
Leu30-Arg278 (Cys33Ser)
Accession #
N-terminal Sequence
Leu30
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
TGFB1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
27 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
28-36 kDa, reducing & 60-70 kDa, non-reducing conditions (variably glycosylated)
Publications
Read Publications using
246-LP in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LAP (TGF-beta 1) Protein

  • CED
  • DPD1
  • LAP (TGFbeta 1)
  • LAP (TGF-beta 1)
  • LAP
  • TGFB
  • TGFB1
  • TGFbeta
  • transforming growth factor beta 1

Background

TGF-  beta 1 (transforming growth factor beta 1) and the closely related TGF-beta 2 and -beta 3 are members of the large TGF-beta  superfamily. TGF‑  beta proteins are highly pleiotropic cytokines that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑3). Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (4). A furin-like convertase processes the proprotein within the trans-Golgi to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-  beta 1 (4‑6). Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex (4‑8). Purified LAP is also capable of associating with active TGF-beta with high affinity, and can neutralize TGF-beta activity (9). Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (5‑7). TGF-beta activation from latency is controlled both spatially and temporally, by multiple pathways that include actions of proteases such as plasmin and MMP9, and/or by thrombospondin 1 or selected integrins (5, 8). The LAP portion of human TGF-beta 1 shares 91%, 92%, 85%, 86% and 88% aa identity with porcine, canine, mouse, rat and equine TGF-beta 1 LAP, respectively, while mature human TGF-beta 1 portion shares 100% aa identity with porcine, canine and bovine TGF-beta 1, and 99% aa identity with mouse, rat and equine TGF-beta 1. Although different isoforms of TGF-beta are naturally associated with their own distinct LAPs, the TGF-beta 1 LAP is capable of complexing with, and inactivating, all other human TGF-beta isoforms and those of most other species (9). Mutations within the LAP are associated with Camurati-Engelmann disease, a rare sclerosing bone dysplasia characterized by inappropriate presence of active TGF-beta 1 (10).

  1. Dunker, N. & K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
  2. Wahl, S.M. (2006) Immunol. Rev. 213:213.
  3. Chang, H. et al. (2002) Endocr. Rev. 23:787.
  4. Derynck, R. et al. (1985) Nature 316:701.
  5. Dabovic, B. and D.B. Rifkin (2008) “TGF-beta Bioavailability” in The TGF-beta Family. Derynck, R. and K. Miyazono (eds): Cold Spring Harbor Laboratory Press, p. 179.
  6. Brunner, A.M. et al. (1989) J. Biol. Chem. 264:13660.
  7. Miyazono, K. et al. (1991) EMBO J. 10:1091.
  8. Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
  9. Miller, D.M. et al. (1992) Mol. Endocrinol. 6:694.
  10. Janssens, K. et al. (2003) J. Biol. Chem. 278:7718.

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246-LP
Species: Hu
Applications: Bioactivity

Publications for LAP (TGF-beta 1) (246-LP)(22)

We have publications tested in 4 confirmed species: Human, Mouse, N/A, Porcine.

We have publications tested in 4 applications: Antibody Production, Bioassay, In Vivo, Surface Plasmon Resonance.


Filter By Application
Antibody Production
(1)
Bioassay
(16)
In Vivo
(2)
Surface Plasmon Resonance
(3)
All Applications
Filter By Species
Human
(12)
Mouse
(7)
N/A
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 22. Show All 22 Publications.
Publications using 246-LP Applications Species
M Zhou, J Niu, J Wang, H Gao, M Shahbaz, Z Niu, Z Li, X Zou, B Liang Integrin &amp;alphav&amp;beta8 serves as a Novel Marker of Poor Prognosis in Colon Carcinoma and Regulates Cell Invasiveness through the Activation of TGF-&amp;beta1 J Cancer, 2020;11(13):3803-3815. 2020 [PMID: 32328185] (Bioassay, Human) Bioassay Human
J Basta, L Robbins, L Stout, MJ Prinsen, DW Griggs, M Rauchman Pharmacologic inhibition of RGD-binding integrins ameliorates fibrosis and improves function following kidney injury Physiol Rep, 2020;8(7):e14329. 2020 [PMID: 32281744] (Bioassay, Human) Bioassay Human
DC Briggs, AWW Langford-S, HL Birchenoug, TA Jowitt, CM Kielty, JJ Enghild, C Baldock, CM Milner, AJ Day Inter-&amp;alpha-inhibitor heavy chain-1 has an integrin-like 3D structure mediating immune regulatory activities and matrix stabilization during ovulation J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32144206] (Surface Plasmon Resonance) Surface Plasmon Resonance
Lynne A Murray The TGF-? inhibitory activity of antibody 37E1B5 depends on its H-CDR2 glycan MAbs, 2016;0(0):0. 2016 [PMID: 27834568] (Bioassay, Mouse) Bioassay Mouse
PSG9 Stimulates Increase in FoxP3+ Regulatory T-Cells through the TGF-?1 Pathway PLoS ONE, 2016;11(7):e0158050. 2016 [PMID: 27389696] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice J. Biol. Chem., 2016;291(15):8070-89. 2016 [PMID: 26663085] (Bioassay, N/A) Bioassay N/A
Turner C, Badu-Nkansah K, Crowley D, van der Flier A, Hynes R alpha5 and alphav integrins cooperate to regulate vascular smooth muscle and neural crest functions in vivo. Development, 2015;142(4):797-808. 2015 [PMID: 25670798] (Bioassay, Mouse) Bioassay Mouse
Fedele C, Singh A, Zerlanko B, Iozzo R, Languino L The alphavbeta6 integrin is transferred intercellularly via exosomes. J Biol Chem, 2015;290(8):4545-51. 2015 [PMID: 25568317] (Bioassay, Human) Bioassay Human
Ballesteros A, Mentink-Kane M, Warren J, Kaplan G, Dveksler G Induction and activation of latent transforming growth factor-beta1 are carried out by two distinct domains of pregnancy-specific glycoprotein 1 (PSG1). J Biol Chem, 2015;290(7):4422-31. 2015 [PMID: 25548275] (Surface Plasmon Resonance, Human) Surface Plasmon Resonance Human
Guha R, Das S, Ghosh J, Sundar S, Dujardin J, Roy S Antimony resistant Leishmania donovani but not sensitive ones drives greater frequency of potent T-regulatory cells upon interaction with human PBMCs: role of IL-10 and TGF-beta in early immune response. PLoS Negl Trop Dis, 2014;8(7):e2995. 2014 [PMID: 25032977] (Bioassay, Human) Bioassay Human
Show All 22 Publications.

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Bioinformatics

Gene Symbol TGFB1
Uniprot