Recombinant Human KGF/FGF-7 Heat Stable Protein, CF

Recombinant Human KGF/FGF-7 Heat Stable Protein (Catalog # BT-FGF7HS) exhibits a higher melting temperature (Tm) than wild-type human KGF/FGF-7. Thermal stability was assessed by Differential Scanning Fluorimetry (DSF).

Recombinant Human KGF/FGF‑7 Heat Stable Protein (Catalog # BT-FGF7HS) induced cell proliferation in Ba/F3 mouse pro-B cells transfected with human FGF RIIb.

Recombinant human FGF-7 protein activates SEAP reporter activity in HEK293 human embryonic kidney cells stably transfected with the secreted alkaline phosphatase (SEAP) reporter gene. (A) Recombinant heat-stable human FGF-7 (Catalog # BT-FGF7HS) and (B) recombinant human KGF/FGF-7 protein were either untreated or incubated at 37 °C for 3 days in cell culture media. Heat-stable (HS) FGF-7 retained comparable bioactivity after incubation relative to the untreated control, indicating increased thermal stability. In contrast, wild type (WT) FGF-7 showed a significant loss of activity after 3 days of heat-stress incubation, suggesting lower thermal stability.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human KGF/FGF-7 Heat Stable Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using Ba/F3 mouse pro B cells transfected with human FGF RIIb. The ED₅₀ for this effect is 6.00-60.0 ng/mL.
• Source: E. coli-derived human KGF/FGF-7 protein
  Proprietary, engineered based on P21781
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• SDS-PAGE: 19-23 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, Na₂SO₄ and EDTA with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human KGF/FGF-7 Heat Stable Protein, CF

• FGF7
• FGF-7
• fibroblast growth factor 7HBGF-7
• HBGF7
• HBGF-7
• Heparin-binding growth factor 7
• keratinocyte growth factor
• KGF
• KGFfibroblast growth factor 7 (keratinocyte growth factor)

Background

KGF (keratinocyte growth factor), also known as FGF-7 (fibroblast growth factor-7), is one of 22 known members of the mouse FGF family of secreted proteins that plays a key role in development, morphogenesis, angiogenesis, wound healing, and tumorigenesis (1-4). KGF expression is restricted to cells of mesenchymal origin. When secreted, it acts as a paracrine growth factor for nearby epithelial cells (1). KGF speeds wound healing by being dramatically upregulated in response to damage to skin or internal structures that results in high local concentrations of inflammatory mediators such as IL-1 and TNF-alpha . (2, 5). KGF promotes cell migration and invasion, and mediates melanocyte transfer to keratinocytes upon UVB radiation (6, 7). It has been used ectopically to avoid chemotherapy-induced oral mucositis in patients with hematological malignancies (1). Deletion of KGF affects kidney development, producing abnormally small ureteric buds and fewer nephrons (8). It also impedes hair follicle differentiation (9). The 194 amino acid (aa) KGF precursor contains a 31 aa signal sequence and, like all other FGFs, an ~120 aa beta -trefoil scaffold that includes receptor- and heparin-binding sites. KGF signals only through the IIIb splice form of the tyrosine kinase receptor, FGF R2 (FGF R2-IIIb/KGF R) (10). Receptor dimerization requires an octameric or larger heparin or heparin sulfate proteoglycan (11). FGF-10, also called KGF2, shares 51% aa identity and similar function to KGF, but shows more limited expression than KGF and uses an additional receptor, FGF R2-IIIc (12). Following receptor engagement, KGF is typically degraded, while FGF-10 is recycled (12). Mature human KGF, which is active across species, shares 98% aa sequence identity with bovine, equine, ovine and canine, 96% with mouse and porcine, and 92% with rat KGF, respectively. Our proprietary Heat Stable FGF-7 (HS) is engineered for superior thermostability compared to the wild-type protein. Additionally, FGF-7 HS retains full bioactivity under heat-challenge conditions in a functional reporter assay.

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