Recombinant Human Keratocan His-tag Protein, CF

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2 μg/lane of Recombinant Human Keratocan His-tag (Catalog # 10838-KE) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Keratocan His-tag Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human Keratocan His-tag is immobilized at 1.00 μg/mL (100 µL/well), Recombinant Human BLMH/Bleomycin Hydrolase  (Catalog # 6200-CY) binds with an ED50 of 1.20-12.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human Keratocan protein
Arg21-Ile352, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Arg21
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
44-62 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Keratocan His-tag Protein, CF

  • CNA2
  • KERA
  • Keratocan
  • KTN
  • SLRR2B
  • SLRR2BKeratan sulfate proteoglycan keratocan

Background

Keratocan is a keratan sulfate-containing proteoglycan that is highly expressed in human cornea (1, 2). It is an extracellular matrix protein that belongs to the class II small leucine-rich proteoglycan family (1). This class of protein contains 10-12 leucine-rich repeat motifs flanked by conserved cysteine residues in N-and C-terminal domains of the core proteins (1, 3). Human keratocan is expressed as a 352 amino acid (aa) protein that includes a 20 aa signal peptide and the 332 aa keratocan protein containing ten LRR motifs (2). Human keratocan shares 87% and 88% aa identity with mouse and rat keratocan, respectively. Keratocan is expressed in the cornea and tendon where it is likely to play a role in collagen fibrillogenesis and in maintaining corneal clarity (4, 5). Keratocan is also expressed in the bone and can modulate osteogenic differentiation (6).
  1. Kao, W. et al. (2003) Ocul Surf. 1:5.
  2. Pellegata, N.S. et al. (2000) Nat. Genet. 25:91.
  3. Ameye, L. et al. (2002) Glycobiology 12:107R.
  4. Liu, C. et al. (2003) J. Biol. Chem. 278:21672.
  5. Rees, S.G. et al. (2009) Osteoarthritis Cartilage 17:276.
  6. Igwe, J.C. et al. (2011) Connect. Tissue Res. 52:401.

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