Recombinant Human IMP Dehydrogenase 2/IMPDH2 Protein, CF

Images

 
1 μg/lane of Recombinant Human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by silverstaining,showing a band at 57 kDa.
Recombinant Human IMPDH2 (Catalog # 8349-DH) is measured byits ability to convert the substrate inosine-5'-phosphate (IMP) toxanthosine-5'-phosphate (XMP). The activity (orange) is approximately 3.5-foldgreater than the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human IMP Dehydrogenase 2/IMPDH2 Protein, CF Summary

Details of Functionality
Measured by its ability to convert the substrate inosine-5'-phosphate (IMP) to xanthosine-5'-phosphate (XMP). The specific activity is >30 pmol/min/μg, as measured under the described conditions.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human IMP Dehydrogenase 2/IMPDH2 protein
Met1-Phe514, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Met1 predicted
Protein/Peptide Type
Recombinant Enzymes
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Enzyme Activity
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
52-61 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Assay Procedure
  • Assay Buffer: 50 mM Tris, 300 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 8.0
  • Recombinant Human Inosine 5'-Monophosphate Dehydrogenase 2/IMPDH2 (rhIMPDH2) (Catalog # 8349-DH)
  • Nicotinamide adenine dinucleotide sodium salt ( beta -NAD) (Sigma, Catalog # N6522), 100 mM stock in deionized water
  • Inosine 5'-monophosphate (IMP) (Sigma, Catalog # I4625), 100 mM stock in deionized water.
  • UV plate (Costar, Catalog # 3635)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhIMPDH2 to 20 μg/mL in Assay Buffer.
  2. Create Substrate Mixture containing 500 μM IMP and 1 mM beta -NAD in Assay Buffer.
  3. Load 50 μL of 20 μg/mL rhIMPDH-2 into a plate, and start the reaction by adding 50 μL of Substrate Mixture. For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of Substrate Mixture.
  4. Read plate at a wavelength of 339 nm (bottom read) in kinetic mode for 5 minutes.
  5. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol
ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg)

 

*Adjusted for Substrate Blank.
**Using the extinction coefficient 6220 M-1cm-1.
***Using the path correction 0.320 cm.
Note: the output of many spectrophotometers is in mOD.

Per Well:
  • rhIMPDH-2: 1.0 μg
  • beta -NAD: 500 μM
  • IMP: 250 μM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IMP Dehydrogenase 2/IMPDH2 Protein, CF

  • EC 1.1.1.205
  • IMDH2
  • IMP (inosine 5'-monophosphate) dehydrogenase 2
  • IMP (inosine monophosphate) dehydrogenase 2
  • IMP Dehydrogenase 2
  • IMP Oxireductase 2
  • IMPD 2
  • IMPD2
  • IMPD2IMP oxireductase 2
  • IMPDH 2
  • IMPDH2
  • IMPDH-II
  • inosine 5' phosphate dehydrogenase 2
  • inosine monophosphate dehydrogenase type II
  • inosine-5'-monophosphate dehydrogenase 2

Background

IMPDH2 (inosine monophosphate dehydrogenase) is one of two cytosolic and nuclear enzyme isoforms that play a central role in guanine nucleotide metabolism. The isoforms are 84% identical but distinctly regulated. While IMPDH1 is generally constitutively expressed, IMPDH2 is inducible during proliferation and transformation (1, 2). Both isoforms form a homotetramer of approximately 55 kDa monomers containing a catalytic barrel domain and a subdomain with two cystathione beta-synthase domains which mediate RNA and DNA binding (1, 3). Both enzymes catalyze the NAD-dependent conversion of inosine monophosphate (IMP) to hypoxanthine monophosphate (XMP) which is a precursor for the synthesis of GMP, guanosine, and guanine. These compounds are critical for DNA synthesis and cell proliferation (4, 5) which explains the importance of IMPDH in cancer and viral infection (6-9). Although IMPDH1 and IMPDH2 are known to be inhibited by the immunosuppressant drug mycophenolic acid (MPA), much research has targeted discovery of additional and selective inhibitors for IMPDH isoforms given they are targets for several major therapeutic areas (1, 9-13). Human IMPDH2 shares 99% amino acid sequence identity with mouse IMPDH2.
  1. Hedstrom, L. (2009) Chem. Rev. 109:2903.
  2. Thomas, E.C. et al. (2012) PLoS One 12:e51096.
  3. McLean, J.E. et al. (2004) Biochem. J. 379:243.
  4. Lane, A.N. and T.W. Fan (2015) Nucleic Acids Res. 43:2466.
  5. Jackson, R.C. et al. (1975) Nature 256:331.
  6. Zho, J. et al. (2015) Med. Oncol. 32:373.
  7. Xu, Y. et al. (2017) Sci. Rep. 7:745.
  8. Nair, V. (2007) Antivir. Chem. Chemother. 18:245.
  9. Trapero, A. et al. (2018) J. Med. Chem. 61:2806.
  10. Barnes, B.J. et al. (2001) Int. J. Cancer 94:275.
  11. Cholewinksi, G. et al. (2015) J. Enzyme Inhib. Med. Chem. 30:550.
  12. Liao, L.X. et al. (2017) Proc. Natl. Acad. Sci. USA 114:E5986.
  13. Cuny, G.D. et al. (2017) Expert Opin. Ther. Pat. 27:677.

Customers Who Viewed This Item Also Viewed...

AF8554
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
NB100-56722
Species: Ca, Hu, Mu, Rb, Rt
Applications: B/N, DB, ELISA, Flow-CS, Flow, Func, ICC/IF, IP, In vitro, WB
H00001072-M04
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB300-270
Species: Ch, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, In vitro, Simple Western, WB
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP2-67667
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NBP1-81293
Species: Hu
Applications: ICC/IF, IHC, IHC-P, KD, WB
AF1245
Species: Mu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, ICFlow, WB
NBP1-90824
Species: Hu
Applications: ICC/IF, WB
NBP1-33527
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-90365
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PLA, WB
NBP1-88296
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
AF3589
Species: Hu, Mu, Rt
Applications: ICC, Simple Western, WB
H00001719-M01
Species: Hu, Mu, Rt, Ye
Applications: ELISA, ICC/IF, S-ELISA, WB
NBP1-87741
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-302
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NB600-101
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
NBP2-03825
Species: Ca, Hu, Pm, Mu, Pm, Rt
Applications: Flow, IHC, IHC-P, WB
8349-DH
Species: Hu
Applications: Enzyme Activity

Publications for IMP Dehydrogenase 2/IMPDH2 (8349-DH) (0)

There are no publications for IMP Dehydrogenase 2/IMPDH2 (8349-DH).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for IMP Dehydrogenase 2/IMPDH2 (8349-DH) (0)

There are no reviews for IMP Dehydrogenase 2/IMPDH2 (8349-DH). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IMP Dehydrogenase 2/IMPDH2 (8349-DH) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IMP Dehydrogenase 2/IMPDH2 Protein, CF and receive a gift card or discount.

Bioinformatics

Uniprot