Cofilin Antibody (1A1)

Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in NIH/3T3 (Cat # L018V1).

Immunocytochemistry/Immunofluorescence: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. Antibody concentration 1.5 ug/ml

Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Western Blot analysis of CFL1 expression in PC-12(Cat # L012V1 ).

Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in HeLa (Cat # L013V1).

Western Blot: Cofilin Antibody (1A1) [H00001072-M04] - Analysis of CFL1 expression in Raw 264.7 (Cat # L024V1).

ELISA: Cofilin Antibody (1A1) [H00001072-M04] - Detection limit for recombinant GST tagged CFL1 is approximately 10ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clone: 1A1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cofilin Antibody (1A1) Summary

• Immunogen: CFL1 (AAH11005, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASGVAVSDGVIKVFNDMKVRKSSTPEEVKKRKKAVLFCLSEDKKNIILEEGKEILVGDVGQTVDDPYATFAKMLPDKDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKLTGIKHELQANCYEGVKDRCTLAEKLGGSAVISLEGKPL
• Specificity: cofilin 1 (non-muscle)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CFL1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA. Use in Immunohistochemistry reported in scientific literature (PMID: 23712570).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Cofilin Antibody (1A1)

• 18 kDa phosphoprotein
• CFL
• cofilin 1 (non-muscle)
• Cofilin, non-muscle isoform
• cofilin-1
• p18

Background

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their pointed (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+- independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cofilin Antibody (H00001072-M04)(4)

Publications using H00001072-M04

• Chang YF, Chao CH, Lin LY et al. Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor. J Biomed Opt. 2014-01-01 [PMID: 23788282]
• Franciosi L, Govorukhina N, Fusetti F et al. Proteomic analysis of human Epithelial Lining Fluid by microfluidics-based nanoLC-MS/MS: a feasibility study. Electrophoresis 2013-09-01 [PMID: 23712570] (IF/IHC, Human)
• Sakai A, Otani M, Miyamoto A et al. Identification of phosphorylated serine-15 and -82 residues of HSPB1 in 5-fluorouracil- resistant colorectal cancer cells by proteomics. Journal of Proteomics. 2011-01-01 [PMID: 21989268]
• Nishimura S, Tsuda H, Kataoka F et al. Overexpression of cofilin 1 can predict progression-free survival in patients with epithelial ovarian cancer receiving standard therapy. Hum Pathol. 2011-01-13 [PMID: 21237490]

Bioinformatics

• Gene Symbol: CFL1
• Entrez:
  • 1072(Human)
• OMIM:
  • 601442(Human)
• Uniprot:
  • AAH11005(Human)