Recombinant Human IL-21R His-tag Avi-tag Protein, CF

When Recombinant Human IL-21 Protein (8879-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-21R His-tag Avi-tag (Catalog # AVI9249) that produces 50% of the optimal binding response is 40.0-240 ng/mL.

2 μg/lane of Biotinylated Recombinant Human IL-21R His-tag Avi-tag Protein (Catalog # AVI9249) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 51-59 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-21R His-tag Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL-21 Protein (Catalog # 8879-IL) is immobilized at 1.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-21R His-tag Avi-tag (Catalog # AVI9247) that produces 50% of the optimal binding response is 40.0-240 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-21R protein
  

  Human IL-21R (Cys20-Pro236) Accession # Q9HBE5.1	6-His tag	Avi-tag
  N-terminus		C-terminus

• Accession #: Q9HBE5.1
• N-terminal Sequence: Cys20
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 51-59 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-21R His-tag Avi-tag Protein, CF

• CD360 antigen
• CD360
• IL-21 R
• IL-21 receptor
• IL21R
• IL-21R
• interleukin 21 receptor
• interleukin-21 receptor
• MGC10967
• NILR
• Novel interleukin receptor

Background

Interleukin-21 Receptor (IL-21 R )is a type I transmembrane glycoprotein within the class I cytokine receptor family (1). IL-21 R associates with the common gamma  chain ( gamma _c) which is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15 (2, 3). Mature human IL-21 R consists of a 213 amino acid (aa) extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSxWS motif, followed by a 21 aa transmembrane domain and a 285 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). Within the ECD, human IL-21 R shares 69% aa identity with mouse and rat IL-21 R, respectively. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 4, 5). Both IL-21 and IL-4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (6). B cell IL-21 R engagement induces Blimp-1 (which mediates plasma cell differentiation) and is important for memory responses (7, 8). IL-21 R engagement enhances NK cell mediated cytotoxic activity and IFN-gamma production (4, 9), control of viral infection and tumor growth by CD8⁺ T cells (10), development of regulatory T cells (11), IL-23 responsiveness of encephalitogenic Th17 cells (12), but suppresses the accumulation of IL-17 secreting gamma δ T cells in the airway (13). IL-21 R expression is often upregulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (14, 15). Our Avi-tag Biotinylated human IL-21R features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Tangye, S.G. (2015) Curr. Opin. Immunol. 34:107.
2. Asao, H. et al. (2001) J. Immunol. 167:1.
3. Habib, T. et al. (2002) Biochemistry 41:8725.
4. Parrish-Novak, et al. (2000) Nature 408:57.
5. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439.
6. Ozaki, K. et al. (2002) Science 298:1630.
7. Rankin, A.L. et al. (2011) J. Immunol. 186:667.
8. King, I.L. et al. (2010) J. Immunol. 185:6138.
9. Kasaian, M.T. et al. (2002) Immunity 16:559.
10. Frohlich, A. et al. (2009 Science 324:1576.
11. Tortola, L. et al. (2010) Blood 116:5200.
12. Lee, Y. et al. (2015) J. Clin. Invest. 125:4011.
13. Moser, E.K. et al. (2015) PLoS One 10:e0120169.
14. Jin, H. et al. (2009) J. Clin. Invest. 119:47.
15. Sarosiek, K.A. et al. (2010) Blood 115:570.

Publications for IL-21R (AVI9249)(1)

Publication using AVI9249

• Chun, JH;Lim, BS;Roy, S;Walsh, MJ;Abhiraman, GC;Zhangxu, K;Atajanova, T;Revach, OY;Clark, EC;Li, P;Palin, CA;Khanna, A;Tower, S;Kureshi, R;Hoffman, MT;Sharova, T;Lawless, A;Cohen, S;Boland, GM;Nguyen, T;Peprah, F;Tello, JG;Liu, SY;Kim, CJ;Shin, H;Quijano-Rubio, A;Jude, KM;Gerben, S;Murray, A;Heine, P;DeWitt, M;Ulge, UY;Carter, L;King, NP;Silva, DA;Kueh, HY;Kalia, V;Sarkar, S;Jenkins, RW;Garcia, KC;Leonard, WJ;Dougan, M;Dougan, SK;Baker, D; Potent antitumor activity of a designed interleukin-21 mimic bioRxiv : the preprint server for biology 2024-12-07 [PMID: 39677740] (Bioassay, N/A)

Bioinformatics

• Uniprot:
  • Q9HBE5.1()