Recombinant Human IL-2 Protein, CF

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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IL-2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05‑0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 9.1 x 106 IU/mg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
202-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid.  Alternatively, reconstitute at 100 μg/mL in sterile deionized water and use within 24 hours, store at 2 to 8 °C.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein, CF

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL/CF)(320)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.


Filter By Application
Bioassay
(279)
CAR-T
(1)
CAR-T (Bioassay)
(2)
CAR-T (Differentiation)
(1)
Cell Culture
(27)
ELISA (Standard)
(4)
In Vivo
(2)
Luminex Development
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(272)
Mouse
(36)
Bat - Pteropus alecto
(1)
Bovine
(1)
Canine
(1)
Chinchilla
(1)
Feline
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 320. Show All 320 Publications.
Publications using 202-IL/CF Applications Species
KO Jang, YW Lee, H Kim, DK Chung Complement Inactivation Strategy of Staphylococcus aureus Using Decay-Accelerating Factor and the Response of Infected HaCaT Cells International Journal of Molecular Sciences, 2021;22(8):. 2021 [PMID: 33924622] (Cell Culture, Human) Cell Culture Human
O Bartok, A Pataskar, R Nagel, M Laos, E Goldfarb, D Hayoun, R Levy, PR Körner, IZM Kreuger, J Champagne, EA Zaal, OB Bleijervel, X Huang, J Kenski, J Wargo, A Brandis, Y Levin, O Mizrahi, M Alon, S Lebon, W Yang, MM Nielsen, N Stern-Gino, M Altelaar, CR Berkers, T Geiger, DS Peeper, J Olweus, Y Samuels, R Agami Anti-tumour immunity induces aberrant peptide presentation in melanoma Nature, 2020;0(0):. 2020 [PMID: 33328638] (Cell Culture, Human) Cell Culture Human
KK Naidoo, ZB Shangase, T Rashid, A Ngubane, N Ismail, T Ndung'u, CF Thobakgale The DARC-null trait is associated with moderate modulation of NK cell profiles and unaltered cytolytic T cell profiles in black South Africans PLoS ONE, 2020;15(11):e0242448. 2020 [PMID: 33211774] (Bioassay, Human) Bioassay Human
MW Grunst, AG Grandea, SK Janaka, I Hammad, P Grimes, JA Karl, R Wiseman, DH O'Connor, DT Evans Functional Interactions of Common Allotypes of Rhesus Macaque Fc&amp;gammaR2A and Fc&amp;gammaR3A with Human and Macaque IgG Subclasses J Immunol, 2020;0(0):. 2020 [PMID: 33208458] (Cell Culture, Human) Cell Culture Human
BMF Winkel, LR Pelgrom, R van Schuij, E Baalbergen, MS Ganesh, H Gerritsma, CM de Korne, N Duszenko, MCC Langenberg, SC Chevalley-, HH Smits, EC de Jong, B Everts, B Franke-Fay, M Roestenber Plasmodium sporozoites induce regulatory macrophages PLoS Pathog., 2020;16(9):e1008799. 2020 [PMID: 32898164] (Cell Culture, Human) Cell Culture Human
M Bachsais, S Salti, K Zaoui, GS Hassan, F Aoudjit, W Mourad CD154 inhibits death of T cells via a Cis interaction with the &amp;alpha5&amp;beta1 integrin PLoS ONE, 2020;15(8):e0235753. 2020 [PMID: 32745080] (Cell Culture, Human) Cell Culture Human
IJ Beccard, L Hofmann, JC Schroeder, S Ludwig, S Laban, C Brunner, R Lotfi, TK Hoffmann, EK Jackson, PJ Schuler, MN Theodoraki Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer Cancers (Basel), 2020;12(7):. 2020 [PMID: 32708274] (Cell Culture, Human) Cell Culture Human
W Gao, S Xu, M Zhang, S Liu, SP Siu, H Peng, JC Ng, GS Tsao, AW Chan, VL Chow, JY Chan, TS Wong NADPH oxidase 5&amp;alpha promotes the formation of CD271 tumor-initiating cells in oral cancer Am J Cancer Res, 2020;10(6):1710-1727. 2020 [PMID: 32642285] (Cell Culture, Human) Cell Culture Human
Y Wan, C Zhang, Y Xu, M Wang, Q Rao, H Xing, Z Tian, K Tang, Y Mi, Y Wang, J Wang Hyperfunction of CD4 CD25 regulatory T cells in de novo acute myeloid leukemia BMC Cancer, 2020;20(1):472. 2020 [PMID: 32456622] (Cell Culture, Human) Cell Culture Human
K Froning, J Maguire, A Sereno, F Huang, S Chang, K Weichert, AJ Frommelt, J Dong, X Wu, H Austin, EM Conner, JR Fitchett, AR Heng, D Balasubram, MT Hilgers, B Kuhlman, SJ Demarest Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics Nat Commun, 2020;11(1):2330. 2020 [PMID: 32393818] (Bioassay, Human) Bioassay Human
Show All 320 Publications.

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Bioinformatics

Gene Symbol IL2
Uniprot