Recombinant Human IL-2 Protein, CF


Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Product Details

Reactivity HuSpecies Glossary
Applications BA

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Recombinant Human IL-2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05‑0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
E. coli-derived
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
Read Publications using
202-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein, CF

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF


Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

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Publications for (207)

We have publications tested in 6 confirmed species: Human, Mouse, Bat - Pteropus alecto, Canine, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, Luminex Development.

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In Vivo
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Filter By Species
Bat - Pteropus alecto
Primate - Macaca mulatta (Rhesus Macaque)
All Species
Showing Publications 1 - 10 of 207. Show All 207 Publications.
Publications using 202-IL/CF Applications Species
S Galland, J Vuille, P Martin, I Letovanec, A Caignard, G Fregni, I Stamenkovi Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets Cell Rep, 2017;20(12):2891-2905. 2017 [PMID: 28930684] (Bioassay, Human) Bioassay Human
L Sarmiento, J De Rosa, A Machicote, LA Billordo, P Baz, PM Fernández, I Kaimen Mac, A Blanco, EI Arana Characterization of tonsillar IL10 secreting B cells and their role in the pathophysiology of tonsillar hypertrophy Sci Rep, 2017;7(1):11077. 2017 [PMID: 28894116] (Bioassay, Human) Bioassay Human
Y Nakayama, J Kosek, L Capone, EM Hur, PH Schafer, GE Ringheim Aiolos Overexpression in Systemic Lupus Erythematosus B Cell Subtypes and BAFF-Induced Memory B Cell Differentiation Are Reduced by CC-220 Modulation of Cereblon Activity J. Immunol., 2017;199(7):2388-2407. 2017 [PMID: 28848067] (Bioassay, Human) Bioassay Human
VA Gall, AV Philips, N Qiao, K Clise-Dwye, AA Perakis, M Zhang, GT Clifton, P Sukhumalch, Q Ma, SM Reddy, D Yu, JJ Molldrem, GE Peoples, G Alatrash, EA Mittendorf Trastuzumab Increases HER2 Uptake and Cross-Presentation by Dendritic Cells Cancer Res., 2017;0(0):. 2017 [PMID: 28819024] (Bioassay, Human) Bioassay Human
Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors Oncol Lett, 2017;14(2):1363-1372. 2017 [PMID: 28808483] (Bioassay, Human) Bioassay Human
A Tovar-Sala, A Weinberg Cytomegalovirus infection in HIV-infected and uninfected individuals is characterized by circulating regulatory T cells of unconstrained antigenic specificity PLoS ONE, 2017;12(7):e0180691. 2017 [PMID: 28683106] (Bioassay, Human) Bioassay Human
U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab PLoS ONE, 2017;12(7):e0180088. 2017 [PMID: 28672038] (Bioassay, Human) Bioassay Human
DL Bolton, K McGinnis, G Finak, P Chattopadh, R Gottardo, M Roederer Combined single-cell quantitation of host and SIV genes and proteins ex vivo reveals host-pathogen interactions in individual cells PLoS Pathog., 2017;13(6):e1006445. 2017 [PMID: 28654687] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque)) Bioassay Primate - Macaca mulatta (Rhesus Macaque)
M Jourdan, N Robert, M Cren, C Thibaut, C Duperray, A Kassambara, M Cogné, K Tarte, B Klein, J Moreaux Characterization of human FCRL4-positive B cells PLoS ONE, 2017;12(6):e0179793. 2017 [PMID: 28636654] (Bioassay, Human) Bioassay Human
A Pavesi, AT Tan, S Koh, A Chia, M Colombo, E Antonecchi, C Miccolis, E Ceccarello, G Adriani, MT Raimondi, RD Kamm, A Bertoletti A 3D microfluidic model for preclinical evaluation of TCR-engineered T cells against solid tumors JCI Insight, 2017;2(12):. 2017 [PMID: 28614795] (Bioassay, Human) Bioassay Human
Show All 207 Publications.

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Gene Symbol IL2