Recombinant Human IL-2 Protein, CF

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Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications BA
Format
Carrier-Free

Order Details

Recombinant Human IL-2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05‑0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Source
E. coli-derived
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
202-IL/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein, CF

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

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Publications for (207)

We have publications tested in 6 confirmed species: Human, Mouse, Bat - Pteropus alecto, Canine, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, Luminex Development.


Filter By Application
Bioassay
(199)
ELISA Standard
(4)
In Vivo
(2)
Luminex Development
(1)
All Applications
Filter By Species
Human
(174)
Mouse
(24)
Bat - Pteropus alecto
(1)
Canine
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 207. Show All 207 Publications.
Publications using 202-IL/CF Applications Species
S Galland, J Vuille, P Martin, I Letovanec, A Caignard, G Fregni, I Stamenkovi Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets Cell Rep, 2017;20(12):2891-2905. 2017 [PMID: 28930684] (Bioassay, Human) Bioassay Human
L Sarmiento, J De Rosa, A Machicote, LA Billordo, P Baz, PM Fernández, I Kaimen Mac, A Blanco, EI Arana Characterization of tonsillar IL10 secreting B cells and their role in the pathophysiology of tonsillar hypertrophy Sci Rep, 2017;7(1):11077. 2017 [PMID: 28894116] (Bioassay, Human) Bioassay Human
Y Nakayama, J Kosek, L Capone, EM Hur, PH Schafer, GE Ringheim Aiolos Overexpression in Systemic Lupus Erythematosus B Cell Subtypes and BAFF-Induced Memory B Cell Differentiation Are Reduced by CC-220 Modulation of Cereblon Activity J. Immunol., 2017;199(7):2388-2407. 2017 [PMID: 28848067] (Bioassay, Human) Bioassay Human
VA Gall, AV Philips, N Qiao, K Clise-Dwye, AA Perakis, M Zhang, GT Clifton, P Sukhumalch, Q Ma, SM Reddy, D Yu, JJ Molldrem, GE Peoples, G Alatrash, EA Mittendorf Trastuzumab Increases HER2 Uptake and Cross-Presentation by Dendritic Cells Cancer Res., 2017;0(0):. 2017 [PMID: 28819024] (Bioassay, Human) Bioassay Human
Z Lu, N Xu, X Zhou, G Gao, L Li, J Huang, Y Li, Q Lu, B He, C Pan, X Liu Therapeutic immune monitoring of CD4(+)CD25(+) T cells in chronic myeloid leukemia patients treated with tyrosine kinase inhibitors Oncol Lett, 2017;14(2):1363-1372. 2017 [PMID: 28808483] (Bioassay, Human) Bioassay Human
A Tovar-Sala, A Weinberg Cytomegalovirus infection in HIV-infected and uninfected individuals is characterized by circulating regulatory T cells of unconstrained antigenic specificity PLoS ONE, 2017;12(7):e0180691. 2017 [PMID: 28683106] (Bioassay, Human) Bioassay Human
U Bughani, A Saha, A Kuriakose, R Nair, RB Sadashivar, R Venkataram, S Patel, AT Deshchougu, SK S, E Montero, HV Pai, DV Palanivelu, R Melarkode, P Nair T cell activation and differentiation is modulated by a CD6 domain 1 antibody Itolizumab PLoS ONE, 2017;12(7):e0180088. 2017 [PMID: 28672038] (Bioassay, Human) Bioassay Human
DL Bolton, K McGinnis, G Finak, P Chattopadh, R Gottardo, M Roederer Combined single-cell quantitation of host and SIV genes and proteins ex vivo reveals host-pathogen interactions in individual cells PLoS Pathog., 2017;13(6):e1006445. 2017 [PMID: 28654687] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque)) Bioassay Primate - Macaca mulatta (Rhesus Macaque)
M Jourdan, N Robert, M Cren, C Thibaut, C Duperray, A Kassambara, M Cogné, K Tarte, B Klein, J Moreaux Characterization of human FCRL4-positive B cells PLoS ONE, 2017;12(6):e0179793. 2017 [PMID: 28636654] (Bioassay, Human) Bioassay Human
A Pavesi, AT Tan, S Koh, A Chia, M Colombo, E Antonecchi, C Miccolis, E Ceccarello, G Adriani, MT Raimondi, RD Kamm, A Bertoletti A 3D microfluidic model for preclinical evaluation of TCR-engineered T cells against solid tumors JCI Insight, 2017;2(12):. 2017 [PMID: 28614795] (Bioassay, Human) Bioassay Human
Show All 207 Publications.

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Bioinformatics

Gene Symbol IL2
Entrez
Uniprot