Recombinant Human IL-2 Protein, CF


1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.

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Recombinant Human IL-2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05‑0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 9.1 x 106 IU/mg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).   Specific activity is for reference purposes only and is not routinely tested.  
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
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202-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid.  Alternatively, reconstitute at 100 μg/mL in sterile deionized water and use within 24 hours, store at 2 to 8 °C.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein, CF

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF


Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL/CF)(320)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.

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Bat - Pteropus alecto
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 320. Show All 320 Publications.
Publications using 202-IL/CF Applications Species
KO Jang, YW Lee, H Kim, DK Chung Complement Inactivation Strategy of Staphylococcus aureus Using Decay-Accelerating Factor and the Response of Infected HaCaT Cells International Journal of Molecular Sciences, 2021;22(8):. 2021 [PMID: 33924622] (Cell Culture, Human) Cell Culture Human
O Bartok, A Pataskar, R Nagel, M Laos, E Goldfarb, D Hayoun, R Levy, PR Körner, IZM Kreuger, J Champagne, EA Zaal, OB Bleijervel, X Huang, J Kenski, J Wargo, A Brandis, Y Levin, O Mizrahi, M Alon, S Lebon, W Yang, MM Nielsen, N Stern-Gino, M Altelaar, CR Berkers, T Geiger, DS Peeper, J Olweus, Y Samuels, R Agami Anti-tumour immunity induces aberrant peptide presentation in melanoma Nature, 2020;0(0):. 2020 [PMID: 33328638] (Cell Culture, Human) Cell Culture Human
KK Naidoo, ZB Shangase, T Rashid, A Ngubane, N Ismail, T Ndung'u, CF Thobakgale The DARC-null trait is associated with moderate modulation of NK cell profiles and unaltered cytolytic T cell profiles in black South Africans PLoS ONE, 2020;15(11):e0242448. 2020 [PMID: 33211774] (Bioassay, Human) Bioassay Human
MW Grunst, AG Grandea, SK Janaka, I Hammad, P Grimes, JA Karl, R Wiseman, DH O'Connor, DT Evans Functional Interactions of Common Allotypes of Rhesus Macaque Fc&amp;gammaR2A and Fc&amp;gammaR3A with Human and Macaque IgG Subclasses J Immunol, 2020;0(0):. 2020 [PMID: 33208458] (Cell Culture, Human) Cell Culture Human
BMF Winkel, LR Pelgrom, R van Schuij, E Baalbergen, MS Ganesh, H Gerritsma, CM de Korne, N Duszenko, MCC Langenberg, SC Chevalley-, HH Smits, EC de Jong, B Everts, B Franke-Fay, M Roestenber Plasmodium sporozoites induce regulatory macrophages PLoS Pathog., 2020;16(9):e1008799. 2020 [PMID: 32898164] (Cell Culture, Human) Cell Culture Human
M Bachsais, S Salti, K Zaoui, GS Hassan, F Aoudjit, W Mourad CD154 inhibits death of T cells via a Cis interaction with the &amp;alpha5&amp;beta1 integrin PLoS ONE, 2020;15(8):e0235753. 2020 [PMID: 32745080] (Cell Culture, Human) Cell Culture Human
IJ Beccard, L Hofmann, JC Schroeder, S Ludwig, S Laban, C Brunner, R Lotfi, TK Hoffmann, EK Jackson, PJ Schuler, MN Theodoraki Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer Cancers (Basel), 2020;12(7):. 2020 [PMID: 32708274] (Cell Culture, Human) Cell Culture Human
W Gao, S Xu, M Zhang, S Liu, SP Siu, H Peng, JC Ng, GS Tsao, AW Chan, VL Chow, JY Chan, TS Wong NADPH oxidase 5&amp;alpha promotes the formation of CD271 tumor-initiating cells in oral cancer Am J Cancer Res, 2020;10(6):1710-1727. 2020 [PMID: 32642285] (Cell Culture, Human) Cell Culture Human
Y Wan, C Zhang, Y Xu, M Wang, Q Rao, H Xing, Z Tian, K Tang, Y Mi, Y Wang, J Wang Hyperfunction of CD4 CD25 regulatory T cells in de novo acute myeloid leukemia BMC Cancer, 2020;20(1):472. 2020 [PMID: 32456622] (Cell Culture, Human) Cell Culture Human
K Froning, J Maguire, A Sereno, F Huang, S Chang, K Weichert, AJ Frommelt, J Dong, X Wu, H Austin, EM Conner, JR Fitchett, AR Heng, D Balasubram, MT Hilgers, B Kuhlman, SJ Demarest Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics Nat Commun, 2020;11(1):2330. 2020 [PMID: 32393818] (Bioassay, Human) Bioassay Human
Show All 320 Publications.

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Gene Symbol IL2