Recombinant Human IL-2 Protein, CF


Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

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Applications Bioactivity

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Recombinant Human IL-2 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05‑0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
Read Publications using
202-IL/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein, CF

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF


Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL/CF)(245)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: Bioassay, ELISA Standard, In Vivo, Luminex Development, SPR.

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Bat - Pteropus alecto
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 245. Show All 245 Publications.
Publications using 202-IL/CF Applications Species
L Guo, Y Hou, L Song, S Zhu, F Lin, Y Bai D-Mannose Enhanced Immunomodulation of Periodontal Ligament Stem Cells via Inhibiting IL-6 Secretion Stem Cells Int, 2018;2018(0):7168231. 2018 [PMID: 30271438] (Bioassay, Human) Bioassay Human
S Krishna, P Ulrich, E Wilson, F Parikh, P Narang, S Yang, AK Read, S Kim-Schulz, JG Park, M Posner, MA Wilson Say, A Sikora, KS Anderson Human papilloma virus specific immunogenicity and dysfunction of CD8+ T cells in head and neck cancer Cancer Res., 2018;0(0):. 2018 [PMID: 30154146] (Bioassay, Human) Bioassay Human
C You, Y Yang, B Gao Imperatorin Targets MCL-1 to Sensitize CD133+ Lung Cancer Cells to ??-T Cell-Mediated Cytotoxicity Cell. Physiol. Biochem., 2018;49(1):235-244. 2018 [PMID: 30138935] (Bioassay, Human) Bioassay Human
SR Kim, JM Lewis, BM Cyrenne, PF Monico, FN Mirza, KR Carlson, FM Foss, M Girardi BET inhibition in advanced cutaneous T cell lymphoma is synergistically potentiated by BCL2 inhibition or HDAC inhibition Oncotarget, 2018;9(49):29193-29207. 2018 [PMID: 30018745] (Bioassay, Human) Bioassay Human
X Zhao, S Sankaran, J Yap, CT Too, ZZ Ho, G Dolton, M Legut, EC Ren, AK Sewell, A Bertoletti, PA MacAry, J Brzostek, NRJ Gascoigne Nonstimulatory peptide-MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling Nat Commun, 2018;9(1):2716. 2018 [PMID: 30006605] (Bioassay, Human) Bioassay Human
M Kim, S Pyo, CH Kang, CO Lee, HK Lee, SU Choi, CH Park Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancer PLoS ONE, 2018;13(6):e0198347. 2018 [PMID: 29874279] (Bioassay, Human) Bioassay Human
M Nag, Y Wang, K De Paris, J E Fogle Histone Modulation Blocks Treg-Induced Foxp3 Binding to the IL-2 Promoter of Virus-Specific CD8? T Cells from Feline Immunodeficiency Virus-Infected Cats Viruses, 2018;10(6):. 2018 [PMID: 29861472] (Bioassay, Feline) Bioassay Feline
H Stamm, F Klingler, EM Grossjohan, J Muschhamme, E Vettorazzi, M Heuser, U Mock, F Thol, G Vohwinkel, E Latuske, C Bokemeyer, R Kischel, C Dos Santos, S Stienen, M Friedrich, M Lutteropp, D Nagorsen, J Wellbrock, W Fiedler Immune checkpoints PVR and PVRL2 are prognostic markers in AML and their blockade represents a new therapeutic option Oncogene, 2018;0(0):. 2018 [PMID: 29855615] (Bioassay, Human) Bioassay Human
CY Kuo, JD Long, B Campo-Fern, S de Oliveir, AR Cooper, Z Romero, MD Hoban, AV Joglekar, GR Lill, ML Kaufman, S Fitz-Gibbo, X Wang, RP Hollis, DB Kohn Site-Specific Gene Editing of Human Hematopoietic Stem Cells for X-Linked Hyper-IgM Syndrome Cell Rep, 2018;23(9):2606-2616. 2018 [PMID: 29847792] (Bioassay, Human) Bioassay Human
A Schmidt, F Marabita, NA Kiani, CC Gross, HJ Johansson, S Éliás, S Rautio, M Eriksson, SJ Fernandes, G Silberberg, U Ullah, U Bhatia, H Lähdesmäki, J Lehtiö, D Gomez-Cabr, H Wiendl, R Lahesmaa, J Tegnér Time-resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3 BMC Biol., 2018;16(1):47. 2018 [PMID: 29730990] (Bioassay, Human) Bioassay Human
Show All 245 Publications.

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Gene Symbol IL2