Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF Summary

Details of Functionality
Measured by its ability to inhibit the IL-18-induced response of KG‑1 human acute myelogenous leukemia cells in the presence of soluble Recombinant Human IL‑18 R beta /IL‑1 R7 Fc Chimera (Catalog # 118-AP). Approximately 5‑15 µg/mL of rhIL-18 Ra/Fc will inhibit 50% of the biological response due to 40 ng/mL of rhIL-18 in the presence of 30 µg/mL of rhIL-18 R beta  (AcPL)/Fc Chimera.
Source
Mouse myeloma cell line, NS0-derived human IL-18 R alpha/IL-1 R5 protein
Human IL-18 R alpha
(Glu20 - Arg329)
Accession # Q13478
IEGRDID Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus


Accession #
N-terminal Sequence
Glu20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL18R1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
63 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95-105 kDa, reducing conditions
Publications
Read Publications using
816-LR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 1 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-18 R alpha/IL-1 R5 Fc Chimera, CF

  • CD218a
  • IL-1 R5
  • IL-1 Rrp
  • IL-18 R alpha
  • IL-18 R1
  • IL18R alpha
  • IL18R1
  • IL-18Ra

Background

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL-18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

  1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
  2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.

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816-LR
Species: Hu
Applications: Bioactivity

Publications for IL-18 R alpha/IL-1 R5 (816-LR)(2)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: ELISA Standard, Surface Plasmon Resonance.


Filter By Application
ELISA Standard
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(1)
N/A
(1)
All Species

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Bioinformatics

Gene Symbol IL18R1
Entrez
Uniprot