Recombinant Human IL-1 RI Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-1 RI Protein Summary

Details of Functionality
Measured by its ability to inhibit IL-1 beta -dependent proliferation in D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. Approximately 0.2-1.0 µg/mL of IL-1 RI will inhibit 50% of the biological response due to 30 pg/mL of recombinant human IL-1 beta.
Source
Mouse myeloma cell line, NS0-derived human IL-1 RI protein
Asp21-Thr332
Accession #
N-terminal Sequence
Asp21
Protein/Peptide Type
Recombinant Proteins
Gene
IL1R1
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55 kDa, reducing conditions
Publications
Read Publications using
269-1R in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 RI Protein

  • CD121 antigen-like family member A
  • CD121a antigen
  • CD121a
  • IL-1 RI
  • IL1R
  • IL1R1
  • IL1RI
  • IL-1RI
  • IL1RT1
  • IL-1RT1
  • IL-1RT-1
  • Interleukin 1 Receptor 1
  • interleukin 1 receptor, type I
  • interleukin receptor 1
  • interleukin-1 receptor type 1
  • p80

Background

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor Type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts, and endothelial cells. IL-1 receptor Type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence identity in their extracellular domains. The two receptor types do not heterodimerize into a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the Type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This Type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor Type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the Type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling Type I receptor complex. Recombinant IL-1 soluble receptor Type I is a potent antagonist of IL-1 action.

  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

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Publications for IL-1 RI (269-1R)(5)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 5 applications: Antibody Absorption, Bioassay, ELISA (Standard), In Vivo, Surface Plasmon Resonance.


Filter By Application
Antibody Absorption
(1)
Bioassay
(1)
ELISA (Standard)
(1)
In Vivo
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(1)
N/A
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using 269-1R Applications Species
Issafras H, Corbin J, Goldfine I, Roell M Detailed mechanistic analysis of gevokizumab, an allosteric anti-IL-1beta antibody with differential receptor-modulating properties. J Pharmacol Exp Ther, 2014;348(1):202-15. 2014 [PMID: 24194526] (Surface Plasmon Resonance, N/A) Surface Plasmon Resonance N/A
Narita M, Shimamura M, Imai S, Kubota C, Yajima Y, Takagi T, Shiokawa M, Inoue T, Suzuki M, Suzuki T Role of interleukin-1beta and tumor necrosis factor-alpha-dependent expression of cyclooxygenase-2 mRNA in thermal hyperalgesia induced by chronic inflammation in mice. Neuroscience, 2007;152(2):477-86. 2007 [PMID: 18262365] (In Vivo, Mouse) In Vivo Mouse
Pfleger C, Schloot N, ter Veld F Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays. J. Immunol. Methods, 2007;329(1):214-8. 2007 [PMID: 17964594] (ELISA (Standard), N/A) ELISA (Standard) N/A
Herrmann-Lavoie C, Rao CV, Akoum A Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells. Endocrinology, 2007;148(11):5377-84. 2007 [PMID: 17702847] (Antibody Absorption, N/A) Antibody Absorption N/A
Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies. Arthritis Rheum., 2007;56(2):674-87. 2007 [PMID: 17265504] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol IL1R1
Uniprot