Recombinant Human GFR alpha-1/GDNF R alpha-1 His Protein, CF

Recombinant Human GFR alpha -1/GDNF R alpha -1 His-tag Protein (Catalog # 11398-GR) binds Recombinant Human GDNF Protein (212-GD/CF) with an ED50 of 26.0-260 ng/mL.

2 μg/lane of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 His-tag Protein (Catalog # 11398-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 51-64 kDa, under reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GFR alpha-1/GDNF R alpha-1 His Protein, CF Summary

• Additional Information: His-tag
• Details of Functionality: Measured by its binding ability in a functional ELISA. Recombinant Human GFR alpha -1/GDNF R alpha -1 His-tag (Catalog # 11398-GR) binds Recombinant Human GDNF Protein (Catalog # 212‑GD/CF) with an ED 50 of 26.0-260 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human GFR alpha-1/GDNF R alpha-1 protein Ala19-Ser429, with a C-terminal 6-His tag
• Accession #: P56159.2
• N-terminal Sequence: Ala19
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 51-64 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GFR alpha-1/GDNF R alpha-1 His Protein, CF

• DKFZp313E1012
• DKFZp686J0156
• GDNF family receptor alpha 1
• GDNF family receptor alpha-1
• GDNF R alpha-1
• GDNF receptor alpha-1
• GDNFRAGFR-ALPHA-1
• GDNFR-alpha-1
• GDNFRMGC23045
• GFR alpha1
• GFR alpha-1
• GFRA1
• GFRa-1
• GFR-alpha-1
• Glial cell line-derived neurotrophic factor receptor alpha
• GPI-linked anchor protein
• PI-linked cell-surface accessory protein
• RET ligand 1
• RET1L
• RETL1FLJ10561
• TGF-beta related neurotrophic factor receptor 1
• TGF-beta-related neurotrophic factor receptor 1
• TRNR1FLJ31546

Background

Glial cell line-derived growth factor (GDNF), neurturin (NTN), artemin and persephin are distant members of the TGF-beta superfamily. They function as neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits [GDNF receptor alpha -1 (GFR alpha -1) or GFR alpha -2]. GFR alpha -1 and -2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Human GFR alpha -1 cDNA encodes a 465 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, human GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% aa identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.

Bioinformatics

• Uniprot:
  • P56159.2()