Recombinant Human GDNF Protein, CF

Equivalent bioactivity of GMP (BT-GDNF-GMP), Animal-Free (BT-GDNF-AFL) and RUO (Catalog # BT-GDNF) grades of Recombinant Human GDNF as measured in a cell proliferation assay using the cell proliferation of SH-SY5Y cells (orange, green, red, respectively).

Recombinant Human GDNF Protein (Catalog # BT-GDNF) stimulates proliferation in the SH-SY5Y human neuroblastoma cell line. The ED50 for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFRa-1/GDNF Ra-1 Fc Chimera Protein (714-GR).

2 μg/lane ofRecombinant Human GDNFProtein (Catalog # BT-GDNF)was resolved with SDS-PAGEunder reducing (R) and nonreducing (NR) conditions andvisualized by Coomassie® Bluestaining, showing a band at 8 kDaunder reducing conditions.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GDNF Protein, CF Summary

• Details of Functionality: Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED₅₀ for this effect is 0.750-9.00 ng/mL in the presence of Recombinant Human GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 714‑GR).
• Source: E. coli-derived human GDNF protein
  Gly113-Ile211
• N-terminal Sequence: Gly113 & Met
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 8 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >97%, by SDS-PAGE with quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute the 10 μg size at 100 μg/mL in water. Reconstitute all other sizes at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GDNF Protein, CF

• Astrocyte-derived trophic factor
• ATF
• ATF1
• ATF2
• GDNF
• glial cell derived neurotrophic factor
• glial cell line derived neurotrophic factor
• glial cell line-derived neurotrophic factor
• HFB1-GDNF
• HGDNF
• HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell‑expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.