Recombinant Human Fibulin 5 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Fibulin 5 Protein, CF Summary

• Additional Information: HEK293-derived
• Details of Functionality: Measured by the ability of the immobilized protein to enhance the adhesion of HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is 15-90 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Fibulin 5/DANCE protein
  Gln24-Phe448, with an N-terminal HA tag
• Accession #: Q9UBX5
• N-terminal Sequence: Tyr
• Protein/Peptide Type: Recombinant Proteins
• Gene: FBLN5
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-72 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA and CHAPS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibulin 5 Protein, CF

• ARMD3fibulin-5
• DANCE
• DANCEFIBL-5
• Developmental arteries and neural crest EGF-like protein
• EVEC
• FBLN5
• Fibulin 5
• UP50
• UP50FLJ90059
• Urine p50 protein

Background

Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2-4). It is downregulated in the adult vasculature but is re-expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alpha V beta 3, alpha V beta 5, alpha 9 beta 1, alpha 4 beta 1, and alpha 5 beta 1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2,
LTBP-4, and the crosslinking lysyl oxidase-like enzymes LOXL1,2, and 4 along Fibrillin microfibrils (6, 9-12). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 13). Depending on the context, Fibulin 5 can function either as a tumor suppressor or enhancer of tumor cell invasiveness (14, 16). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 15).

1. Papke, C.L. and H. Yanagisawa (2014) Matrix Biol. 37:142.
2. Nakamura, T. et al. (1999) J. Biol. Chem. 274:22476.
3. Kowal, R.C. et al. (1999) Circ. Res. 84:1166.
4. Kuang, P.-P. et al. (2003) Am. J. Physiol. Lung Cell. Mol. Physiol. 285:L1147.
5. Spencer, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:2946.
6. Nakamura, T. et al. (2002) Nature 415:171.
7. Lomas, A.C. et al. (2007) Biochem. J. 405:417.
8. Yanagisawa, H. et al. (2002) Nature 415:168.
9. Wachi, H. et al. (2008) J. Biochem. 143:633.
10. Hirai, M. et al. (2007) J. Cell Biol. 176:1061.
11. Hirai, M. et al. (2007) EMBO J. 26:3283.
12. Noda, K. et al. (2013) Proc. Natl. Acad. Sci. USA 110:2852.
13. Sullivan, K.M. et al. (2007) Lab. Invest. 87:818.
14. Lee, Y.-H. et al. (2008) Carcinogenesis 29:2243.
15. Loeys, B. et al. (2002) Hum. Mol. Genet. 11:2113.
16. Yue, W. et al. (2009) Cancer Res. 69:6339.

Publications for Fibulin 5/DANCE (9006-FB)(1)

Publication using 9006-FB

• The Different Binding Properties of Cultured Human Corneal Endothelial Cell Subpopulations to Descemet&#039;s Membrane Components Invest Ophthalmol Vis Sci, 2016-09-01;57(11):4599-605. 2016-09-01 [PMID: 27598865] (Bioassay, Human)

Bioinformatics

• Gene Symbol: FBLN5
• Uniprot:
  • Q9UBX5()