Recombinant Human Fibulin 5 Protein, CF

Images

 
There are currently no images for Fibulin 5/DANCE (9006-FB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Fibulin 5 Protein, CF Summary

Additional Information
HEK293-derived
Details of Functionality
Measured by the ability of the immobilized protein to enhance the adhesion of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 15-90 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human Fibulin 5/DANCE protein
Gln24-Phe448, with an N-terminal HA tag
Accession #
N-terminal Sequence
Tyr
Protein/Peptide Type
Recombinant Proteins
Gene
FBLN5
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-72 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, EDTA and CHAPS with Trehalose.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibulin 5 Protein, CF

  • ARMD3fibulin-5
  • DANCE
  • DANCEFIBL-5
  • Developmental arteries and neural crest EGF-like protein
  • EVEC
  • FBLN5
  • Fibulin 5
  • UP50
  • UP50FLJ90059
  • Urine p50 protein

Background

Fibulin 5, also known as DANCE and EVEC, is a secreted 55 kDa matricellular glycoprotein that plays an important role in elastic fiber network assembly and angiogenesis (1). Mature human Fibulin 5 contains an N-terminal EGF-like domain with an RGD motif, a 44 amino acid (aa) spacer region, five more tandem EGF-like domains, and a 115 aa Fibulin-like C-terminal region (2, 3). Mature human Fibulin 5 shares 95% aa sequence identity with mouse and rat Fibulin 5. Fibulin 5 is expressed by smooth muscle cells and endothelial cells of the developing vasculature as well as by migrating neural crest cells and lung interstitial fibroblasts (2-4). It is downregulated in the adult vasculature but is re-expressed at aortic branching points, in the uterus, and at sites of mechanical or atherosclerotic injury (2, 3, 5). The RGD motif of Fibulin 5 binds to several cell surface Integrins including alpha V beta 3, alpha V beta 5, alpha 9 beta 1, alpha 4 beta 1, and alpha 5 beta 1 (2, 6, 7). The calcium-dependent binding of Fibulin 5 to elastic fibers serves to anchor cells to the extracellular matrix (8). Fibulin 5 promotes elastic fiber assembly and maturation by organizing Tropoelastin, LTBP-2,
LTBP-4, and the crosslinking lysyl oxidase-like enzymes LOXL1,2, and 4 along Fibrillin microfibrils (6, 9-12). In aged mice with decreased tissue elasticity, proteolytic removal of the N-terminal EGF-like domain prevents Fibulin 5 from interacting with Fibrillin-1 microfibrils (10). Fibulin 5 functions as an angiogenesis inhibitor by inhibiting vascular smooth muscle proliferation and migration and by limiting vascular sprouting (5, 13). Depending on the context, Fibulin 5 can function either as a tumor suppressor or enhancer of tumor cell invasiveness (14, 16). Defects in Fibulin 5 expression or function can result in a loss of connective tissue integrity, cardiac elasticity, and ability to remodel the vasculature after injury (8, 5, 15).
  1. Papke, C.L. and H. Yanagisawa (2014) Matrix Biol. 37:142.
  2. Nakamura, T. et al. (1999) J. Biol. Chem. 274:22476.
  3. Kowal, R.C. et al. (1999) Circ. Res. 84:1166.
  4. Kuang, P.-P. et al. (2003) Am. J. Physiol. Lung Cell. Mol. Physiol. 285:L1147.
  5. Spencer, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:2946.
  6. Nakamura, T. et al. (2002) Nature 415:171.
  7. Lomas, A.C. et al. (2007) Biochem. J. 405:417.
  8. Yanagisawa, H. et al. (2002) Nature 415:168.
  9. Wachi, H. et al. (2008) J. Biochem. 143:633.
  10. Hirai, M. et al. (2007) J. Cell Biol. 176:1061.
  11. Hirai, M. et al. (2007) EMBO J. 26:3283.
  12. Noda, K. et al. (2013) Proc. Natl. Acad. Sci. USA 110:2852.
  13. Sullivan, K.M. et al. (2007) Lab. Invest. 87:818.
  14. Lee, Y.-H. et al. (2008) Carcinogenesis 29:2243.
  15. Loeys, B. et al. (2002) Hum. Mol. Genet. 11:2113.
  16. Yue, W. et al. (2009) Cancer Res. 69:6339.

Customers Who Viewed This Item Also Viewed...

NB100-2076
Species: Hu, Po, Ca, Fe, Gt, GP, Sh
Applications: WB, IHC, IHC-P
NB110-8146
Species: Hu, Po, Bv, Fe
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IM
NB100-2527
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
H00004016-D01P
Species: Hu
Applications: WB, ICC/IF
NBP1-33479
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-77040
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF
NBP1-84724
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-52918
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, KD
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-91258
Species: Hu, Mu, Bv, Ca, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF5945
Species: Hu, Mu, Rt
Applications: WB
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF143
Species: Hu
Applications: WB, Simple Western, IHC
AF3850
Species: Hu
Applications: WB
9006-FB
Species: Hu
Applications: Bioactivity

Publications for Fibulin 5/DANCE (9006-FB) (0)

There are no publications for Fibulin 5/DANCE (9006-FB).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Fibulin 5/DANCE (9006-FB) (0)

There are no reviews for Fibulin 5/DANCE (9006-FB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Fibulin 5/DANCE (9006-FB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Bioinformatics Tool for Fibulin 5/DANCE (9006-FB)

Discover related pathways, diseases and genes to Fibulin 5/DANCE (9006-FB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fibulin 5/DANCE (9006-FB)

Discover more about diseases related to Fibulin 5/DANCE (9006-FB).
 

Pathways for Fibulin 5/DANCE (9006-FB)

View related products by pathway.

PTMs for Fibulin 5/DANCE (9006-FB)

Learn more about PTMs related to Fibulin 5/DANCE (9006-FB).

Blogs on Fibulin 5/DANCE

There are no specific blogs for Fibulin 5/DANCE, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Fibulin 5 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FBLN5
Uniprot