Fibulin-3/EFEMP1 Antibody

Images

 
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and human muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Fibulin 3 Antigen Affinity-purified Polyclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Fibulin-3/EFEMP1 Antibody Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Fibulin 3
Gln19-Phe493
Accession # Q12805
Specificity
Detects human Fibulin 3 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
EFEMP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fibulin-3/EFEMP1 Antibody

  • DHRD
  • EFEMP1
  • EGF containing fibulin-like extracellular matrix protein 1
  • EGF-containing fibulin-like extracellular matrix protein 1
  • Extracellular protein S1-5
  • FBLN3
  • FBLN3DRAD
  • FBNLFLJ35535
  • FIBL-3
  • Fibrillin-like protein
  • fibrillin-like
  • Fibulin 3
  • fibulin-3
  • MGC111353
  • MLVT
  • MTLV
  • S1-5

Background

Fibulin 3 (also S1-5 and EFEMP1) is a secreted, monomeric 55‑57 kDa member of the fibulin family of proteins. In mouse, Fibulin 3 appears in an O-glycosylated 63 and 80 kDa form. Given the high amino acid (aa) conservation between mouse and human, human Fibulin 3 may also demonstrate like isoforms. Fibulin 3 is expressed by fibroblasts, endothelial cells and prechondrocytes, and appears to provide both stability to ECM (elastin and basement membrane), and to regulate cell maturation/ differentiation. Fibulin 3 is reported to activate the EGF receptor, an effect that may influence multiple cell types under multiple scenarios. Mature human Fibulin 3 is 476 aa in length. It contains six EGF-like domains (aa 26‑378), at least one of which binds to the EGF receptor and induces EGFR phosphorylation. There is at least one alternative start site at Met107 that generates a 43 kDa short form. Full-length human Fibulin 3 shares 95% aa identity with mouse Fibulin 3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB3095
Species: Hu
Applications: WB, IHC
NBP1-33479
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-86286
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-84725
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-84724
Species: Hu
Applications: WB, IHC, IHC-P
NB100-2076
Species: Hu, Po, Ca, Fe, Gt, GP, Sh
Applications: WB, IHC, IHC-P
NBP1-30032
Species: Hu, Mu, Bv, Xp
Applications: WB, IHC, IHC-Fr
NBP2-29464
Species: Hu, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NB300-164
Species: Hu, Po, Ca, Rt(-)
Applications: WB, ICC/IF, IHC-Fr, IHC-P, IP
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB200-193
Species: Hu, Mu, Rt, Ch, GP
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF6235
Species: Hu
Applications: WB

Publications for Fibulin-3/EFEMP1 Antibody (AF6235) (0)

There are no publications for Fibulin-3/EFEMP1 Antibody (AF6235).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Fibulin-3/EFEMP1 Antibody (AF6235) (0)

There are no reviews for Fibulin-3/EFEMP1 Antibody (AF6235). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Fibulin-3/EFEMP1 Antibody (AF6235) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Fibulin-3/EFEMP1 Products

Bioinformatics Tool for Fibulin-3/EFEMP1 Antibody (AF6235)

Discover related pathways, diseases and genes to Fibulin-3/EFEMP1 Antibody (AF6235). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fibulin-3/EFEMP1 Antibody (AF6235)

Discover more about diseases related to Fibulin-3/EFEMP1 Antibody (AF6235).
 

Pathways for Fibulin-3/EFEMP1 Antibody (AF6235)

View related products by pathway.

PTMs for Fibulin-3/EFEMP1 Antibody (AF6235)

Learn more about PTMs related to Fibulin-3/EFEMP1 Antibody (AF6235).

Blogs on Fibulin-3/EFEMP1

There are no specific blogs for Fibulin-3/EFEMP1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Fibulin-3/EFEMP1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol EFEMP1
Entrez
Uniprot