Recombinant Human Fibronectin Fragment 4 Protein, CF

Images

 
There are currently no images for Fibronectin (3624-FN).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Fibronectin Fragment 4 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is 0.05-0.35 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Fibronectin protein
Val1913-Glu2477, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Val1913
Protein/Peptide Type
Recombinant Proteins
Gene
FN1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
63.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-95 kDa, reducing conditions
Publications
Read Publication using
3624-FN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibronectin Fragment 4 Protein, CF

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin (FN) is a large modular glycoprotein that is found in a polymeric fibrillar network in the extracellular matrix (ECM). It also forms a soluble disulfide-linked dimeric protomers in plasma and other body fluids (1,2). The protein subunit is made up of three types of homologous structural motifs termed FN type I, type II, and type III repeats (3-5). Alternative splicing generates multiple isoforms of fibronectin which may have insertions of extra type III domains (EDA and EDB) or alteration of the type III connecting segment (IIICS) (5). Fibronectin is a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, and integrins. It is involved in multiple cellular processes including cell adhesion/migration, blood clotting, morphogenesis, tissue repair, and cell signaling. Fibronectin functions are mediated by the insoluble polymeric fibrils in the ECM. Conversion of soluble fibronectin to fibronectin fibrils in the ECM is initiated by binding to cell surface integrins, resulting in exposure of cryptic epitopes necessary for polymerization (1). FN1.4 contains one type III domain, the IIICS domain, three type I domains, and the site of interchain disulfide linkage. Within FN1.4, human fibronectin shares 91% and 88% aa sequence identity with mouse and rat fibronectin, respectively. FN1.4 contains regions that enable association with heparin and fibrin. The IIICS domain contains two sites (CS1 and CS2) that interact with integrin alpha 4 beta 1 (6-8). The CS1 sequence is not accessible in full length fibronectin but is exposed by protease digestion, thereby enabling cell adhesion via integrin alpha 4 beta 1 (9). This is distinct from integrin alpha 5 beta 1-mediated adhesion through an RGD motif located N-terminal to FN1.4 (10). Differential splicing within the IIICS domain determines the presence of CS1 and CS2 sequences and the sensitivity to proteases (6,11).

  1. Mao, Y. and J.E. Schwarzbauer (2005) Matrix Biol. 24:389.
  2. Potts, J.R. and I.D. Campbell (1996) Matrix Biol. 15:313.
  3. Bernard, M.P. et al. (1985) Biochemistry 24:2698.
  4. Kornblihtt, A.R. et al. (1983) Proc. Natl. Acad. Sci. 80:3218.
  5. Kornblihtt, A.R. et al. (1985) EMBO J. 4:1755.
  6. Mould, A.P. et al. (1991) J. Biol. Chem. 266:3579.
  7. Komoriya, A., et al. (1991) J. Biol. Chem. 266:15075.
  8. Wayner, E.A., et al. (1989) J. Cell Biol. 109:1321.
  9. Ugarova, T.P. et al. (1996) Biochemistry 35:10913.
  10. Pytela, R. et al. (1985) Cell 40:191.
  11. Abe, Y. et al. (2005) Biochem. Biophys. Res. Commun. 338:1640.

Customers Who Viewed This Item Also Viewed...

NBP2-67511
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP2-67327
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB110-68136
Species: Hu, Mu, Rt, Fe
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, Flow-IC
NBP2-36561
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, B/N
NBP2-67360
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB600-930
Species: Hu, Rt
Applications: WB, ELISA
NBP1-47386
Species: Hu, Mu, Rb(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, Flow-IC
NBP2-79843
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, CyTOF-ready
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-724
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
MMP200
Species: Hu, Mu, Rt, Po, Ca
NB600-586
Species: Hu, Mu, Ca, Fe
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-22203
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
3624-FN
Species: Hu
Applications: Bioactivity

Publications for Fibronectin (3624-FN)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Fibronectin (3624-FN) (0)

There are no reviews for Fibronectin (3624-FN). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Fibronectin (3624-FN) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Fibronectin Products

Bioinformatics Tool for Fibronectin (3624-FN)

Discover related pathways, diseases and genes to Fibronectin (3624-FN). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Fibronectin (3624-FN)

Discover more about diseases related to Fibronectin (3624-FN).
 

Pathways for Fibronectin (3624-FN)

View related products by pathway.

PTMs for Fibronectin (3624-FN)

Learn more about PTMs related to Fibronectin (3624-FN).

Blogs on Fibronectin.

Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Fibronectin Fragment 4 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol FN1
Uniprot