Recombinant Human Fc mu R/FAIM3 Protein, CF

When human IgM is immobilized at 2 μg/mL, 100 μL/well,Recombinant Human Fc mu R/FAIM3 (Catalog # 9494‑MU) binds with an ED50 of 0.4‑2 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Fc mu R/FAIM3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When human IgM is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Fc mu R/FAIM3 binds with an ED₅₀ of 0.4-2 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human Fc mu R/FAIM3 protein
  

  Human Fc mu R/FAIM3 (Arg18-Gly251) Accession # O60667-1	HisHis	HA (YPYDVPDYA)
  N-terminus		C-terminus

• Accession #: O60667-1
• N-terminal Sequence: Arg18
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 27 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 39-50 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc mu R/FAIM3 Protein, CF

• FAIM3
• Fas apoptotic inhibitory molecule 3
• Fc mu R
• Fc mu receptor
• FCMR
• immunoglobulin mu Fc receptor
• Regulator of Fas-induced apoptosis Toso
• Toso
• TOSOIgM Fc receptor

Background

The human FAIM3 gene (also known as FCMR or TOSO), is a transmembrane sialoglycoprotein expressed mainly by lymphocytes. FAIM3 is a type I membrane protein with an intracellular C-terminal domain and an extracellular N-terminal domain (1). The extracellular domain has homology to the immunoglobulin variable region domains (1) and FAIM3 is identified as an Fc receptor for IgM (2, 3). The amino acid sequence of human FAIM3 is 58% and 55% identical to that of mouse and rat FAIM3, respectively. FAIM3 was shown to be over-expressed in chronic lymphocytic leukemia (CLL) (4) and associated with disease progression (5, 6). FAIM3 has also been linked to the homeostasis and activation of the innate immune system (7). Interestingly, there is growing evidence that neurodegenerative diseases are associated with the activation of the immune surveillance system. This system is responsible for controlling danger signals and responding accordingly to the magnitude and duration of the threat (8, 9).

1. Song, Y. et al (2005) J. Biol. Chem. 280(10):9618.
2. Shima H et al. (2010) Int. Immunol. 22:149.
3. Kubagawa H et al. (2009) J. Exp. Med. 206:2779.
4. Proto-Siqueira R et al. (2008) Blood, 112:394.
5. Pallasch, C et al. (2008) Blood, 112:4213.
6. Pallasch, C P et al (2009) Leukemia & lymphoma, 50:498.
7. Sigruener, A et al (2007) Biochemical and biophysical research communications, 359:723.
8. Richards R I et al (2016) Front. Neurosci. 10:193.
9. Planells-Ferrer L et al (2016) J Neurochem. 139:11.

Publications for FAIM3 (9494-MU)(1)

Publication using 9494-MU

• EY Wang, T Mao, J Klein, Y Dai, JD Huck, F Liu, NS Zheng, T Zhou, B Israelow, P Wong, C Lucas, J Silva, JE Oh, E Song, ES Perotti, S Fischer, M Campbell, JB Fournier, AL Wyllie, CBF Vogels, IM Ott, CC Kalinich, ME Petrone, AE Watkins, CD Cruz, SF Farhadian, WL Schulz, ND Grubaugh, AI Ko, A Iwasaki, AM Ring Diverse Functional Autoantibodies in Patients with COVID-19 medRxiv : the preprint server for health sciences, 2021-02-01;0(0):. 2021-02-01 [PMID: 33330894] (ELISA Capture, Mouse)

Bioinformatics

• Uniprot:
  • O60667-1()