Recombinant Human Fc gamma RIIA His Alexa Fluor® 488 Protein

Streptavidin coated beads conjugated to biotinylated anti-human Fc gamma RIIA/CD32a Antibody were stained with the indicated concentrations of Recombinant Human Fc gamma RIIA/CD32a (R167) His-tag Alexa Fluor® 488 (Catalog # AFG1330).

2 μg/lane of Recombinant Human Fc gamma RIIA/CD32a (R167) His-tag Alexa Fluor® 488 Protein (Catalog # AFG1330) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 26-36 kDa.

• Applications: Bioactivity

Recombinant Human Fc gamma RIIA His Alexa Fluor® 488 Protein Summary

• Additional Information: (R167)
• Details of Functionality: Measured by flow cytometry for its ability to bind anti-human Fc gamma RIIA/CD32a Antibody conjugated beads. The concentration of Recombinant Human Fc gamma RIIA/CD32a (R167) His-tag Alexa Fluor® 488 (Catalog # AFG1330) that produces 50% of the binding response is 20.0-200 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Fc gamma RIIA/CD32a protein
  Ala36-Ile218, with a C-terminal 10-His tag
• Accession #: AAA35827.1
• N-terminal Sequence: Ala36
• Structure / Form: Labeled with Alexa Fluor® 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515–545 nm
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 26-36 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fc gamma RIIA His Alexa Fluor® 488 Protein

• CD32 antigen
• CD32a
• CD32MGC23887
• CDw32fc-gamma-RIIa
• Fc fragment of IgG, low affinity IIa, receptor (CD32)
• Fc fragment of IgG, low affinity IIa, receptor for (CD32)
• Fc gamma RIIA
• FCG2
• Fc-gamma RII-a
• Fc-gamma-RIIa
• FcGR
• FCGR2
• FCGR2A
• FCGR2A1
• FcgRIIA
• FCRIIA
• fcRII-a
• IGFR2MGC30032
• IgG Fc receptor II-a
• Immunoglobulin G Fc receptor II
• low affinity immunoglobulin gamma Fc region receptor II-a

Background

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). The activating-type receptor either has or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high-affinity receptor that binds monomeric IgG, both Fc gamma RII and RIII are low-affinity receptors that bind aggregated or immune complexed IgG (IC). Three genes for human Fc gamma RII (A, B, and C) and one for mouse (Fc gamma RIIB), encoding type I transmembrane proteins with ITAM motifs (Fc gamma RII A and C) or ITIM motifs (Fc gamma RIIB) in their cytoplasmic domains, have been identified (1-3). The extracellular domain of human Fc gamma RIIA shares approximately 90% amino acid sequence homology with human Fc gamma RIIB and Fc gamma RIIC. Fc gamma RIIA is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells) where inhibitory ITIM-bearing receptors may also be coexpressed and co-engaged by specific ligands. Signaling through Fc gamma RIIA results in the initiation of inflammatory responses (cytolysis, phagocytosis, degranulation and cytokine production) that can be modulated by signals from the inhibitory receptors. The strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors. Besides IC, Fc gamma RII A also binds C-reactive protein (CRP) (4, 5). Two allelic variants (R167 and H167) of Fc gamma RIIA that differ in their ability to ligate human IgG2 or CRP exist. The H167 allele has been found to have a protective effect against lupus nephritis.

1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
4. Chi, M. et al. (2002) J. Immunol. 168:1413.
5. Zuniga, R. et al. (2003) Arthritis Rheum. 48:460.

FAQs for Fc gamma RIIA/CD32a (AFG1330). (Showing 1 - 1 of 1 FAQ).

1. Does any of your FCGR2A antibodies (19C10, 6D11, 13G9) cross react to FCGR2B?
   • For these antibodies, we have used the full-length protein FCGR2A (NP_067674) as the immunogen and we have not mapped the epitope that is detected with each Ab yet. I did an alignment of these two proteins and they are highly conserved thus it seems likely that there will be cross-reactivity.

Bioinformatics

• Uniprot:
  • AAA35827.1()