Recombinant Human Erythropoietin R Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Erythropoietin R Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Epo-dependent proliferation of TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 1-4 ng/mL in the presence of 0.1 U/mL of recombinant human Epo.
• Source: Mouse myeloma cell line, NS0-derived human Erythropoietin R protein
  

  Human Epo R (Ala25-Pro250) Accession # P19235	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: P19235
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPOR
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51.4 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Erythropoietin R Fc Chimera Protein, CF

• EpoR
• EPO-R
• Erythropoietin R
• erythropoietin receptor
• MGC138358

Background

Erythropoietin (EPO), a glycoprotein produced primarily by the kidney, is the principal factor that regulates erythropoiesis by stimulating the proliferation and differentiation of erythroid progenitor cells. The biological effects of EPO are mediated by the erythropoietin receptor (EPO R). The genes for human and mouse EPO R have been cloned and characterized. The full-length human EPO R cDNA encodes a type I membrane-spanning protein with 508 amino acid (aa) residues (a 24 aa residue hydrophobic signal sequence, a 226 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 236 aa residue cytoplasmic domain). At the protein sequence level, the human EPO R is approximately 82% identical to the mouse protein. As a result of alternative splicing of the EPO R gene, cDNA clones encoding a truncated form of the EPO R as well as a soluble form of EPO R has been found. The presence of a soluble form of the EPO R has also been detected in human sera. Recombinant soluble EPO R binds EPO with high affinity and is a potent EPO antagonist.

1. Barber, D.L. and A.D. D’Andrea (1992) Seminars in Hematology 29:293.
2. Youssoufian, H. et al. (1993) Blood 9:2223.
3. Lodish, H.F. et al. (1995) Cold Spring Harbor Symposia on Quantitative Biology LX:93.
4. Baynes, R.D. et al. (1993) Blood 82:2088.

Publications for Erythropoietin R (963-ER)(5)

Publications using 963-ER

• J Yang, RK Sumbria The concentration of brain homogenates with the Amicon Ultra Centrifugal filters MethodsX, 2021-11-18;8(0):101584. 2021-11-18 [PMID: 35004217] (ELISA Capture, Mouse)
• B Cho, SJ Yoo, SY Kim, CH Lee, YI Lee, SR Lee, C Moon Second-generation non-hematopoietic erythropoietin-derived peptide for neuroprotection Redox Biology, 2021-12-21;49(0):102223. 2021-12-21 [PMID: 34953452] (Surface Plasmon Resonance, Human)
• DR Burrill, Burrill D, A Vernet, Vernet A, JJ Collins, PA Silver, JC Way, Way J Targeted erythropoietin selectively stimulates red blood cell expansion in vivo. Proc Natl Acad Sci U S A, 2016-04-25;113(19):5245-50. 2016-04-25 [PMID: 27114509] (Enzyme Assay, Human)
• Khankin EV, Mutter WP, Tamez H Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease. PLoS ONE, 2010-02-16;5(2):e9246. 2010-02-16 [PMID: 20169072]
• Lupia E, Bosco O, Goffi A Thrombopoietin contributes to enhanced platelet activation in cigarette smokers. Atherosclerosis, 2009-11-20;210(1):314-9. 2009-11-20 [PMID: 20006331] (Bioassay, Human)

Bioinformatics

• Gene Symbol: EPOR
• Uniprot:
  • P19235()