ErbB2/Her2 Antibody [Unconjugated]

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ErbB2/Her2 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human ErbB2/Her2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1129) at 1.7 µg/mL ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

ErbB2/Her2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2
Thr23-Thr652
Accession # P04626
Specificity
Detects human ErbB2/Her2 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse ErbB2 is observed and less than 5% cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ERBB2
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Inhibition of Cell Growth 0.06-0.3 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 3 Reviews rated 4
using
AF1129 in the following applications:

Publications
Read Publications using
AF1129 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody [Unconjugated]

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB2 is widely expressed in epithelial cells and has also been found to be over-expressed in a large number of breast carcinomas. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other members of the ErbB family (ErbB1 (EGFR), ErbB3, ErbB4) to form higher affinity signaling complexes. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. Interestingly, this same three amino acid signal has also been found in ErbB1 and ErbB4. Phosphoinositide 3-kinase has been shown to play a role in ErbB2 signal transduction. The cytoplasmic domain of ErbB2 has been shown to associate with beta-catenin and plakoglobin. Human ErbB2 consists of 1255 amino acids (aa) with a 21 aa signal sequence, a 631 aa extracellular domain, a 23 aa transmembrane region, and a 580 aa cytoplasmic domain. ErbB2 can be shed from the cell surface by proteolytic cleavage by an unidentified protease. ErbB2 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation (1-10).

  1. Coussens, L. et. al. (1985) Science 230:1132.
  2. Yamamoto, T. et. al. (1986) Nature 319:230.
  3. Kanai, Y. et. al. (1995) Biochem. Biophys. Res. Commun. 208:1067.
  4. Codony-Servat, J. et. al. (1999) Cancer Res. 59:1196.
  5. Carraway, K.L. 3rd et. al.  (1994) J. Biol. Chem. 269:14303.
  6. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Schlessinger, J. (2000) Cell 103:211.
  9. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB2/Her2 Antibody (AF1129)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ELISA Development, Flow Cytometry, Immunoprecipitation, Western Blot.


Filter By Application
ELISA Development
(1)
Flow Cytometry
(2)
Immunoprecipitation
(2)
Western Blot
(3)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1129 Applications Species
AS Nikitina, AV Lipatova, AO Goncharov, AA Kliuchniko, MA Pyatnitski, KG Kuznetsova, A Hamad, PO Vorobyev, ON Alekseeva, M Mahmoud, Y Shakiba, KS Anufrieva, GP Arapidi, MV Ivanov, IA Tarasova, MV Gorshkov, PM Chumakov, SA Moshkovski Multiomic Profiling Identified EGF Receptor Signaling as a Potential Inhibitor of Type I Interferon Response in Models of Oncolytic Therapy by Vesicular Stomatitis Virus International Journal of Molecular Sciences, 2022;23(9):. 2022 [PMID: 35563635] (Western Blot, Human) Western Blot Human
H Luan, TA Bailey, RJ Clubb, BC Mohapatra, AM Bhat, S Chakrabort, N Islam, I Mushtaq, MD Storck, SM Raja, V Band, H Band CHIP/STUB1 Ubiquitin Ligase Functions as a Negative Regulator of ErbB2 by Promoting Its Early Post-Biosynthesis Degradation Cancers, 2021;13(16):. 2021 [PMID: 34439093] (Western Blot, Human) Western Blot Human
J Zhang, LTH Nguyen, R Hickey, N Walters, X Wang, KJ Kwak, LJ Lee, AF Palmer, E Reátegui Immunomagnetic sequential ultrafiltration (iSUF) platform for enrichment and purification of extracellular vesicles from biofluids Scientific Reports, 2021;11(1):8034. 2021 [PMID: 33850163] (Flow Cytometry, Human) Flow Cytometry Human
J Zhang, S Liu, Q Li, Y Shi, Y Wu, F Liu, S Wang, MY Zaky, W Yousuf, Q Sun, D Guo, T Wang, Y Zhang, Y Wang, M Li, H Liu The deubiquitylase USP2 maintains ErbB2 abundance via counteracting endocytic degradation and represents a therapeutic target in ErbB2-positive breast cancer Cell Death Differ., 2020;0(0):. 2020 [PMID: 32327714] (Immunoprecipitation, Human) Immunoprecipitation Human
X Li, CG Nelson, RR Nair, L Hazlehurst, T Moroni, P Martinez-A, AR Nanna, D Hymel, TR Burke, C Rader Stable and Potent Selenomab-Drug Conjugates Cell Chem Biol, 2017;24(4):433-442.e6. 2017 [PMID: 28330604] (Flow Cytometry, Human) Flow Cytometry Human
Denisova G, Jenkins K, Evelegh C, Bramson J Novel method for differentiation between Trastuzumab and host adaptive response. Mol. Immunol., 2011;48(15):1882-5. 2011 [PMID: 21645926] (Western Blot, Human) Western Blot Human
Sarup J, Jin P, Turin L, Bai X, Beryt M, Brdlik C, Higaki JN, Jorgensen B, Lau FW, Lindley P, Liu J, Ni I, Rozzelle J, Kumari R, Watson SA, Zhang J, Shepard HM Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts. Mol. Cancer Ther., 2008;7(10):3223-36. 2008 [PMID: 18852126] (ELISA Development, Human) ELISA Development Human
Kunii K, Davis L, Gorenstein J, Hatch H, Yashiro M, Di Bacco A, Elbi C, Lutterbach B FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival. Cancer Res., 2008;68(7):2340-8. 2008 [PMID: 18381441] (Immunoprecipitation, Human) Immunoprecipitation Human

Reviews for ErbB2/Her2 Antibody (AF1129) (3) 43

Average Rating: 4
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Goat.

Reviews using AF1129:
Filter by Applications
ELISA
(1)
WB
(1)
IF
(1)
All Applications
Filter by Species
Human
(2)
Goat
(1)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
ELISA Goat 08/28/2017
View

Summary

ApplicationELISA
Sample TestedBreast cancer tissue
SpeciesGoat
LotILE316101
  4
reviewed by:
Anonymous -
WB Human 01/05/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22393464
SpeciesHuman
  3
reviewed by:
Anonymous -
IF Human 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 25225290
SpeciesHuman

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FAQs for ErbB2/Her2 Antibody (AF1129). (Showing 1 - 1 of 1 FAQs).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

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Blogs on ErbB2/Her2.

NPC1: A Potential Target For Triple-Negative Breast Cancer
By Natalia Gurule, PhD Breast Cancer is a Heterogeneous DiseaseBreast cancer is the most frequently identified malignancy in women, accounting for 30% of diagnosed cases of cancer in women in the US annuall...  Read full blog post.

Hypoxia-Dependent CAR Stabilizing Construct in T cells Improves Solid Tumor Targeting and Efficacy
By Victoria Osinski, PhDDespite advances in the development of cancer immunotherapies, those specifically targeting tumors still remains limited. Currently, there is great interest in utilizing chimeric antigen rece...  Read full blog post.

Harnessing Natural Killer Cell Activity for Anti-Tumor Immunotherapy
By Victoria Osinski, PhDWhat’s “Natural” About Natural Killer (NK) Cells?For immunologists, the term cytotoxicity often conjures up images of an army of antigen specific CD8+ T cells deploying to ...  Read full blog post.

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Recent Reviews

4
5
1
4
1
3
1
2
0
1
0

 
Verified Customer
08/28/2017
Application: ELISA
Species: Goat

 
Anonymous -
01/05/2015
Application: WB
Species: Human

 
Anonymous -
01/05/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol ERBB2
Uniprot