Ephrin-A5 Antibody [Unconjugated]

Ephrin‑A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Ephrin‑A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3743) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Ephrin-A5 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ephrin-A5
  Gln21-Asn203
  Accession # P52803
• Specificity: Detects human Ephrin-A5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-B3, rmEphrin-A4, rmEphrin-B1 and rmEphrin-B2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EFNA5

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-A5 Antibody [Unconjugated]

• AF1
• AL-1
• EFL-5
• EFNA5
• EPH-related receptor tyrosine kinase ligand 7
• EphrinA5
• Ephrin-A5
• EPLG7
• GLC1M
• LERK-7
• LERK7EFL5
• RAGS

Background

Ephrin-A5, also known as AL-1, RAGS, LERK-7, and EFL-5 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A5 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of murine and human Ephrin-A5 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Ephrin-A5 Antibody (AF3743)(10)

Publications using AF3743

• EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy Mol Med Rep, 2016;0(0):. 2016 [PMID: 27959424] (IHC, Mouse)
• Szepietowska, Barbara, Horvath, Tamas L, Sherwin, Robert S Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia. Diabetes, 2014;63(3):1140-7. 2014 [PMID: 24222347] (Western Blot, Rat)
• Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia. Diabetes, 2013;62(4):1282-8. 2013 [PMID: 23274893] (Western Blot, Rat)
• Henion T, Madany P, Faden A, Schwarting G beta3GnT2 null mice exhibit defective accessory olfactory bulb innervation. Mol Cell Neurosci, 2013;52(0):73-86. 2013 [PMID: 23006775] (IHC-Fr, Mouse)
• Li JJ, Liu DP, Liu GT, Xie D EphrinA5 acts as a tumor suppressor in glioma by negative regulation of epidermal growth factor receptor. Oncogene, 2009;28(15):1759-68. 2009 [PMID: 19270726] (ICC, Human)
• Yumoto N, Wakatsuki S, Kurisaki T, Hara Y, Osumi N, Frisen J, Sehara-Fujisawa A Meltrin beta/ADAM19 interacting with EphA4 in developing neural cells participates in formation of the neuromuscular junction. PLoS ONE, 2008;3(10):e3322. 2008 [PMID: 18830404] (IHC-Fr, Mouse)
• Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping. Neuron, 2008;59(5):746-58. 2008 [PMID: 18786358] (IHC-Fr, Mouse)
• Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y BIG-2 mediates olfactory axon convergence to target glomeruli. Neuron, 2008;57(6):834-46. 2008 [PMID: 18367085] (IHC, Mouse)
• Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets. Cell, 2007;129(2):359-70. 2007 [PMID: 17448994] (IHC, Mouse)
• Janes PW, Saha N, Barton WA, Kolev MV, Wimmer-Kleikamp SH, Nievergall E, Blobel CP, Himanen JP, Lackmann M, Nikolov DB Adam meets Eph: an ADAM substrate recognition module acts as a molecular switch for ephrin cleavage in trans. Cell, 2005;123(2):291-304. 2005 [PMID: 16239146] (Western Blot, Human)

Bioinformatics

• Gene Symbol: EFNA5
• Uniprot:
  • P52803()