Recombinant Human EphA2 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human EphA2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rhEphA2 at 2 µg/mL (100 µL/well) can bind rmEphrin-A1/Fc Chimera with a linear range of 0.078-5 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human EphA2 protein
  Gln25-Asn534, with a C-terminal 6-His tag
• Accession #: P29317
• N-terminal Sequence: No results obtained: Gln25 predicted & Lys27
• Protein/Peptide Type: Recombinant Proteins
• Gene: EPHA2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 56.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-75 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EphA2 Protein, CF

• ARCC2
• EC 2.7.10
• EC 2.7.10.1
• Eck
• ECKepithelial cell receptor protein tyrosine kinase
• EPH receptor A2
• EphA2
• ephrin type-A receptor 2
• Epithelial cell kinase
• Myk2
• Sek2
• soluble EPHA2 variant 1
• Tyrosine-protein kinase receptor ECK

Background

EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1, 2, 3, 4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90 - 94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).

1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419. 
3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
5. Bohme, B et al. (1993) Oncogene 8:2857.
6. Pandey, A. et al. (1995) Science 268:567.
7. Bartley, T.D. et al. (1994) Nature 368:558.
8. Pandey, A. et al. (1994) J. Biol. Chem. 269:30154.
9. Miao, H. et al. (2001) Nat. Cell Biol. 3:527.
10. Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793.
11. Dohn, M. et al. (2001) Oncogene 20:6503.
12. Zelinski, D.P. et al. (2001) Cancer Res. 61:2301.
13. Brantley, D.M. et al. (2002) Oncogene 21:7011.
14. Ogawa, K. et al. (2000) Oncogene 19:6043.
15. Sulman, E.P. et al. (1997) Genomics 40:371.

Publications for EphA2 (3035-A2)(10)

Publications using 3035-A2

• T Fricke, AK Gro beta kopf, A Ensser, M Backovic, AS Hahn Antibodies Targeting KSHV gH/gL Reveal Distinct Neutralization Mechanisms Viruses, 2022-03-05;14(3):. 2022-03-05 [PMID: 35336948] (Bioassay, Human)
• JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-03-10;0(0):101817. 2022-03-10 [PMID: 35278434] (ELISA Capture, N/A)
• L Teo, AG Boghdadi, J Homman-Lud, IC Mundinano, WC Kwan, JA Bourne Replicating infant-specific reactive astrocyte functions in the injured adult brain Progress in neurobiology, 2021-06-17;0(0):102108. 2021-06-17 [PMID: 34147584] (Bioassay, Human)
• M Mukai, N Suruga, N Saeki, K Ogawa EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes BMC Cell Biol., 2017-08-29;18(1):28. 2017-08-29 [PMID: 28851287] (Bioassay, Human)
• M Takasugi, R Okada, A Takahashi, D Virya Chen, S Watanabe, E Hara Small extracellular vesicles secreted from senescent cells promote cancer cell proliferation through EphA2 Nat Commun, 2017-06-06;8(0):15729. 2017-06-06 [PMID: 28585531] (Bioassay, Human)
• Park S, Park S, Kim D, Pyo A, Kimura R, Sathirachinda A, Choy H, Min J, Gambhir S, Hong Y Isolation and Characterization of a Monobody with a Fibronectin Domain III Scaffold That Specifically Binds EphA2. PLoS ONE, 2015-07-15;10(7):e0132976. 2015-07-15 [PMID: 26177208] (Flow Cytometry, Yeast)
• Liao-Chan , Sindy, Daine-Matsuoka , Barbara, Heald , Nathan, Wong , Tiffany, Lin , Tracey, Cai , Allen G, Lai , Michelle, D'Alessio , Joseph A, Theunissen , Jan-Will Quantitative assessment of antibody internalization with novel monoclonal antibodies against Alexa fluorophores. PLoS ONE, 2015-04-20;10(4):e0124708. 2015-04-20 [PMID: 25894652] (Bioassay, Human)
• Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi&#039;s sarcoma-associated herpesvirus. Nat. Med., 2012-06-01;18(6):961-6. 2012-06-01 [PMID: 22635007] (In Vivo, Neutralization, Human)
• Wang QS, Xiang Y, Cui YL, Lin KM, Zhang XF Dietary blue pigments derived from genipin, attenuate inflammation by inhibiting LPS-induced iNOS and COX-2 expression via the NF-kappaB inactivation. PLoS ONE, 2012-03-30;7(3):e34122. 2012-03-30 [PMID: 22479539] (Immunization, Human)
• Jackson D, Gooya J, Mao S, Kinneer K, Xu L, Camara M, Fazenbaker C, Fleming R, Swamynathan S, Meyer D, Senter PD, Gao C, Wu H, Kinch M, Coats S, Kiener PA, Tice DA A human antibody-drug conjugate targeting EphA2 inhibits tumor growth in vivo. Cancer Res., 2008-11-15;68(22):9367-74. 2008-11-15 [PMID: 19010911] (Binding Assay)

Bioinformatics

• Gene Symbol: EPHA2
• Uniprot:
  • P29317()