Recombinant Human Dkk-1 Protein, CF


There are currently no images for Dkk-1 (5439-DK/CF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Human Dkk-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK/CF) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036‑WN). The ED50 for this effect is 10-60 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dkk-1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
25.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
33-38 kDa, reducing conditions
Read Publications using
5439-DK/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Dkk-1 Protein, CF

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like


Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

Customers Who Viewed This Item Also Viewed...

Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Species: Hu
Applications: WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: BA, BA
Species: Hu
Applications: CyTOF-ready, Flow, WB
Species: Hu
Applications: BA
Species: Mu
Applications: BA
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Species: Mu
Applications: IHC, WB
Species: Hu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), ELISA, IHC
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: BA, BA
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Species: Hu, Mu, Pm, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, Simple Western, WB
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Hu
Applications: Bioactivity

Publications for Dkk-1 (5439-DK/CF)(86)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Rabbit.

We have publications tested in 4 applications: Antibody Absorption, Bioassay, Cell Culture, In Vivo.

Filter By Application
Antibody Absorption
Cell Culture
In Vivo
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 86. Show All 86 Publications.
Publications using 5439-DK/CF Applications Species
M Rickman, M Ghim, K Pang, AC von Huelse, EM Drudi, M Sureda-Viv, N Ayoub, V Tajadura-O, SJ George, PD Weinberg, CM Warboys Disturbed flow increases endothelial inflammation and permeability via a Frizzled-4-beta-catenin-dependent pathway Journal of Cell Science, 2023-03-24;0(0):. 2023-03-24 [PMID: 36846872] (Bioassay, Human) Bioassay Human
L Feige, T Kozaki, G Dias de Me, V Guillemot, F Larrous, F Ginhoux, H Bourhy Susceptibilities of CNS Cells towards Rabies Virus Infection Is Linked to Cellular Innate Immune Responses Viruses, 2022-12-29;15(1):. 2022-12-29 [PMID: 36680128] (Bioassay, Human) Bioassay Human
ESY Ng, N Kady, J Hu, A Dave, Z Jiang, J Pei, MB Gorin, A Matynia, RA Radu Membrane Attack Complex Mediates Retinal Pigment Epithelium Cell Death in Stargardt Macular Degeneration Cells, 2022-11-02;11(21):. 2022-11-02 [PMID: 36359858] (Bioassay, Human) Bioassay Human
K Nagano, K Yamana, H Saito, R Kiviranta, AC Pedroni, D Raval, C Niehrs, F Gori, R Baron R-spondin 3 deletion induces Erk phosphorylation to enhance Wnt signaling and promote bone formation in the appendicular skeleton Elife, 2022-11-02;11(0):. 2022-11-02 [PMID: 36321691] (Bioassay, Mouse) Bioassay Mouse
Y Song, Z Wang, J Jiang, Y Piao, Q Bai, Q Piao, L Li, C Xu, H Liu, H Piao, L Li, G Yan miR-181-5p attenuates neutrophilic inflammation in asthma by targeting DEK International immunopharmacology, 2022-09-14;112(0):109243. 2022-09-14 [PMID: 36115279] (Bioassay, Human) Bioassay Human
C Kornsuthis, A Chansaenro, J Manokawinc, KA Tompkins, N Pirarat, T Osathanon Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells Scientific Reports, 2022-05-09;12(1):7583. 2022-05-09 [PMID: 35534526] (Cell Culture, Human) Cell Culture Human
TF Amaral, JGV de Grazia, LAG Martinhao, F De Col, LGB Siqueira, JHM Viana, PJ Hansen Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium Scientific Reports, 2022-05-07;12(1):7503. 2022-05-07 [PMID: 35525843] (Cell Culture, Bovine) Cell Culture Bovine
T Suda, T Yamashita, H Sunagozaka, H Okada, K Nio, Y Sakai, T Yamashita, E Mizukoshi, M Honda, S Kaneko Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma International Journal of Molecular Sciences, 2022-03-03;23(5):. 2022-03-03 [PMID: 35269944] (Bioassay, Human) Bioassay Human
B Ghosh, K Nishida, L Chandrala, S Mahmud, S Thapa, C Swaby, S Chen, AA Khosla, J Katz, VK Sidhaye Epithelial plasticity in COPD results in cellular unjamming due to an increase in polymerized actin Journal of Cell Science, 2022-02-24;0(0):. 2022-02-24 [PMID: 35118497] (Cell Culture, Human) Cell Culture Human
R Kunimatsu, A Kimura, S Sakata, Y Tsuka, Y Yoshimi, T Abe, I Kado, Y Yashima, J Izumino, A Nakatani, M Kitagawa, M Miyauchi, T Takata, K Tanimoto Effects of baicalin on the proliferation and expression of OPG and RANKL in human cementoblast-lineage cells Journal of dental sciences, 2021-06-12;17(1):162-169. 2021-06-12 [PMID: 35028034] (Bioassay, Human) Bioassay Human
Show All 86 Publications.

Reviews for Dkk-1 (5439-DK/CF) (0)

There are no reviews for Dkk-1 (5439-DK/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Dkk-1 (5439-DK/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human Dkk-1 Protein, CF and receive a gift card or discount.


Gene Symbol DKK1