Recombinant Human Dkk-1 Protein, CF


1 μg/lane of Recombinant Human Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 33-38 kDa. Multiple bands in gel are due to variable glycosylation.
Recombinant Human Wnt‑3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-1 (Catalog #5439‑DK/CF) inhibits a constant dose of more

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Dkk-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK/CF) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036‑WN). The ED50 for this effect is 10-60 ng/mL.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dkk-1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
25.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
33-38 kDa, reducing conditions
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5439-DK/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Dkk-1 Protein, CF

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like


Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

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Applications: Bioactivity

Publications for Dkk-1 (5439-DK/CF)(62)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 3 applications: Antibody Absorption, Bioassay, In Vivo.

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Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using 5439-DK/CF Applications Species
S Schivo, S Khurana, K Govindaraj, J Scholma, J Kerkhofs, L Zhong, X Huang, J van de Pol, R Langerak, AJ van Wijnen, L Geris, M Karperien, JN Post ECHO, the executable CHOndrocyte: A computational model to study articular chondrocytes in healthy and disease Cell. Signal., 2019;0(0):109471. 2019 [PMID: 31837466] (Bioassay, Human) Bioassay Human
M Haas, JL Gómez Vázq, DI Sun, HT Tran, M Brislinger, A Tasca, O Shomroni, K Vleminckx, P Walentek δN-Tp63 Mediates Wnt/ β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia Cell Rep, 2019;28(13):3338-3352.e6. 2019 [PMID: 31553905] (Bioassay, Human) Bioassay Human
JM Segal, D Kent, DJ Wesche, SS Ng, M Serra, B Oulès, G Kar, G Emerton, SJI Blackford, S Darmanis, R Miquel, TV Luong, R Yamamoto, A Bonham, W Jassem, N Heaton, A Vigilante, A King, R Sancho, S Teichmann, SR Quake, H Nakauchi, ST Rashid Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors Nat Commun, 2019;10(1):3350. 2019 [PMID: 31350390] (Bioassay, Human) Bioassay Human
OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue Nat Protoc, 2019;14(6):1884-1925. 2019 [PMID: 31110298] (Bioassay, Human) Bioassay Human
O Lyros, AK Lamprecht, L Nie, R Thieme, K Götzel, M Gasparri, G Haasler, P Rafiee, R Shaker, I Gockel Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett&#039;s associated esophageal adenocarcinoma Am J Cancer Res, 2019;9(2):330-346. 2019 [PMID: 30906632] (Bioassay, Human) Bioassay Human
S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12 J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30903650] (Bioassay, Human) Bioassay Human
F Tsurumi, S Baba, D Yoshinaga, K Umeda, T Hirata, J Takita, T Heike The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient PLoS ONE, 2019;14(3):e0213768. 2019 [PMID: 30875388] (Bioassay, Human) Bioassay Human
M Pontremoli, M Brioschi, R Baetta, S Ghilardi, C Banfi Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells Sci Rep, 2018;8(1):16671. 2018 [PMID: 30420710] (Bioassay, Human) Bioassay Human
IF LaRocque-d, JDB Rocha, MP Nunes, PAV Oliveira, DO Nascimento, L Freire-de-, CM Takiya, A Morrot, D Decote-Ric, JO Previato, GA DosReis, L Mendonça-P, CG Freire-de- Involvement of the capsular GalXM-induced IL-17 cytokine in the control of Cryptococcus neoformans infection Sci Rep, 2018;8(1):16378. 2018 [PMID: 30401972] (Bioassay, Human) Bioassay Human
H Takeuchi, S Inagaki, W Morozumi, Y Nakano, Y Inoue, Y Kuse, T Mizoguchi, S Nakamura, M Funato, H Kaneko, H Hara, M Shimazawa VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush Sci Rep, 2018;8(1):16443. 2018 [PMID: 30401804] (Bioassay, Human) Bioassay Human
Show All 62 Publications.

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Gene Symbol DKK1