Recombinant Human Dkk-1 Protein, CF

Images

 
1 μg/lane of Recombinant Human Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 33-38 kDa. Multiple bands in gel are due to variable glycosylation.
Recombinant Human Wnt‑3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-1 (Catalog #5439‑DK/CF) inhibits a constant dose of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human Dkk-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK/CF) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036‑WN). The ED50 for this effect is 10-60 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dkk-1 protein
Thr32-His266
Accession #
N-terminal Sequence
Thr32
Protein/Peptide Type
Recombinant Proteins
Gene
DKK1
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
25.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
33-38 kDa, reducing conditions
Publications
Read Publications using
5439-DK/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Dkk-1 Protein, CF

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

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Publications for Dkk-1 (5439-DK/CF)(80)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 4 applications: Antibody Absorption, Bioassay, Cell Culture, In Vivo.


Filter By Application
Antibody Absorption
(1)
Bioassay
(72)
Cell Culture
(5)
In Vivo
(1)
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Human
(68)
Mouse
(6)
Rat
(1)
Bovine
(2)
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Showing Publications 1 - 10 of 80. Show All 80 Publications.
Publications using 5439-DK/CF Applications Species
Y Song, Z Wang, J Jiang, Y Piao, Q Bai, Q Piao, L Li, C Xu, H Liu, H Piao, L Li, G Yan miR-181-5p attenuates neutrophilic inflammation in asthma by targeting DEK International immunopharmacology, 2022;112(0):109243. 2022 [PMID: 36115279] (Bioassay, Human) Bioassay Human
C Kornsuthis, A Chansaenro, J Manokawinc, KA Tompkins, N Pirarat, T Osathanon Non-canonical Wnt signaling participates in Jagged1-induced osteo/odontogenic differentiation in human dental pulp stem cells Scientific Reports, 2022;12(1):7583. 2022 [PMID: 35534526] (Cell Culture, Human) Cell Culture Human
TF Amaral, JGV de Grazia, LAG Martinhao, F De Col, LGB Siqueira, JHM Viana, PJ Hansen Actions of CSF2 and DKK1 on bovine embryo development and pregnancy outcomes are affected by composition of embryo culture medium Scientific Reports, 2022;12(1):7503. 2022 [PMID: 35525843] (Cell Culture, Bovine) Cell Culture Bovine
T Suda, T Yamashita, H Sunagozaka, H Okada, K Nio, Y Sakai, T Yamashita, E Mizukoshi, M Honda, S Kaneko Dickkopf-1 Promotes Angiogenesis and is a Biomarker for Hepatic Stem Cell-like Hepatocellular Carcinoma International Journal of Molecular Sciences, 2022;23(5):. 2022 [PMID: 35269944] (Bioassay, Human) Bioassay Human
B Ghosh, K Nishida, L Chandrala, S Mahmud, S Thapa, C Swaby, S Chen, AA Khosla, J Katz, VK Sidhaye Epithelial plasticity in COPD results in cellular unjamming due to an increase in polymerized actin Journal of Cell Science, 2022;0(0):. 2022 [PMID: 35118497] (Cell Culture, Human) Cell Culture Human
R Kunimatsu, A Kimura, S Sakata, Y Tsuka, Y Yoshimi, T Abe, I Kado, Y Yashima, J Izumino, A Nakatani, M Kitagawa, M Miyauchi, T Takata, K Tanimoto Effects of baicalin on the proliferation and expression of OPG and RANKL in human cementoblast-lineage cells Journal of dental sciences, 2022;17(1):162-169. 2022 [PMID: 35028034] (Bioassay, Human) Bioassay Human
M Dahlmann, A Monks, ED Harris, D Kobelt, M Osterland, F Khaireddin, P Herrmann, W Kemmner, S Burock, W Walther, RH Shoemaker, U Stein Combination of Wnt/beta-Catenin Targets S100A4 and DKK1 Improves Prognosis of Human Colorectal Cancer Cancers, 2021;14(1):. 2021 [PMID: 35008201] (Bioassay, Human) Bioassay Human
M Kowalski-J, H Schihada, A Turku, T Huber, TP Sakmar, G Schulte Frizzled BRET sensors based on bioorthogonal labeling of unnatural amino acids reveal WNT-induced dynamics of the cysteine-rich domain Science Advances, 2021;7(46):eabj7917. 2021 [PMID: 34757789] (Bioassay, Human) Bioassay Human
L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021;22(19):. 2021 [PMID: 34639189] (Bioassay, Human) Bioassay Human
HJ Wu, A Landshamme, EK Stamenova, A Bolondi, H Kretzmer, A Meissner, F Michor Topological isolation of developmental regulators in mammalian genomes Nature Communications, 2021;12(1):4897. 2021 [PMID: 34385432] (Bioassay, Human) Bioassay Human
Show All 80 Publications.

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Bioinformatics

Gene Symbol DKK1
Uniprot