Recombinant Human Dkk-1 Protein, CF

Images

 
1 μg/lane of Recombinant Human Dkk-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 33-38 kDa. Multiple bands in gel are due to variable glycosylation.
Recombinant Human Wnt‑3a (Catalog # 5036-WN) induces a dose responsive increase in Wnt reporter activity in HEK293 cells (green circles). Recombinant Human Dkk-1 (Catalog #5439‑DK/CF) inhibits a constant dose of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human Dkk-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Human Dkk-1 (Catalog # 5439-DK/CF) inhibits a constant dose of 500 ng/mL of Recombinant Human Wnt-3a (Catalog # 5036‑WN). The ED50 for this effect is 10-60 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dkk-1 protein
Thr32-His266
Accession #
N-terminal Sequence
Thr32
Protein/Peptide Type
Recombinant Proteins
Gene
DKK1
Purity
>95%, by SDS-PAGE with silver staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
25.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
33-38 kDa, reducing conditions
Publications
Read Publications using
5439-DK/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Dkk-1 Protein, CF

  • dickkopf (Xenopus laevis) homolog 1
  • dickkopf homolog 1 (Xenopus laevis)
  • dickkopf related protein-1
  • Dickkopf-1
  • dickkopf-related protein 1
  • Dkk1
  • Dkk-1
  • hDkk-1
  • SKdickkopf-1 like

Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Each domain contains ten cysteine residues (1‑3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 87%, 90% and 91% aa sequence identity with mouse, rat, rabbit and bovine Dkk-1, respectively. It also shares 42% and 36% aa identity with human Dkk-2 and Dkk-4, respectively. Dkk-1 and Dkk-4 are well documented antagonists of the canonical Wnt signaling pathway (1, 2). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP5 or LRP6 (4). Dkk-1 antagonizes Wnt by forming ternary complexes of LRP5/6 with Kremen1 or Kremen2 (4, 5). Dkk‑1/LRP6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 5). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (6, 7). Other sites of expression include developing neurons, hair follicles and the retina of the eye (8, 9). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (10). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (8, 11). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (12).

  1. Krupnik, V.E. et al. (1999) Gene 238:301.  
  2. Niehrs, C. (2006) Oncogene 25:7469. 
  3. Bullock, C.M. et al. (2004) Mol. Pharmacol. 65:582. 
  4. Mao, B. et al. (2001) Nature 411:321. 
  5. Mao, B. et al. (2002) Nature 417:664.  
  6. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  7. Adamska, M. et al. (2004) Dev. Biol. 272:134.
  8. Li, J. et al. (2006) Bone 36:754.
  9. Verani, R. et al. (2006) J. Neurochem. 101:242.
  10. Pinzone, J.J. et al. (2009) Blood 113:517.
  11. Morvan, F. et al. (2006) J. Bone Miner. Res. 21:934.
  12. Cappuccio, I. et al. (2005) J. Neurosci. 25:2647.

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5439-DK/CF
Species: Hu
Applications: Bioactivity

Publications for Dkk-1 (5439-DK/CF)(62)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 3 applications: Antibody Absorption, Bioassay, In Vivo.


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Antibody Absorption
(1)
Bioassay
(59)
In Vivo
(1)
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Human
(52)
Mouse
(5)
Rat
(1)
Bovine
(1)
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(2)
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Showing Publications 1 - 10 of 62. Show All 62 Publications.
Publications using 5439-DK/CF Applications Species
S Schivo, S Khurana, K Govindaraj, J Scholma, J Kerkhofs, L Zhong, X Huang, J van de Pol, R Langerak, AJ van Wijnen, L Geris, M Karperien, JN Post ECHO, the executable CHOndrocyte: A computational model to study articular chondrocytes in healthy and disease Cell. Signal., 2019;0(0):109471. 2019 [PMID: 31837466] (Bioassay, Human) Bioassay Human
M Haas, JL Gómez Vázq, DI Sun, HT Tran, M Brislinger, A Tasca, O Shomroni, K Vleminckx, P Walentek δN-Tp63 Mediates Wnt/ β-Catenin-Induced Inhibition of Differentiation in Basal Stem Cells of Mucociliary Epithelia Cell Rep, 2019;28(13):3338-3352.e6. 2019 [PMID: 31553905] (Bioassay, Human) Bioassay Human
JM Segal, D Kent, DJ Wesche, SS Ng, M Serra, B Oulès, G Kar, G Emerton, SJI Blackford, S Darmanis, R Miquel, TV Luong, R Yamamoto, A Bonham, W Jassem, N Heaton, A Vigilante, A King, R Sancho, S Teichmann, SR Quake, H Nakauchi, ST Rashid Single cell analysis of human foetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors Nat Commun, 2019;10(1):3350. 2019 [PMID: 31350390] (Bioassay, Human) Bioassay Human
OC Tysoe, AW Justin, T Brevini, SE Chen, KT Mahbubani, AK Frank, H Zedira, E Melum, K Saeb-Parsy, AE Markaki, L Vallier, F Sampazioti Isolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue Nat Protoc, 2019;14(6):1884-1925. 2019 [PMID: 31110298] (Bioassay, Human) Bioassay Human
O Lyros, AK Lamprecht, L Nie, R Thieme, K Götzel, M Gasparri, G Haasler, P Rafiee, R Shaker, I Gockel Dickkopf-1 (DKK1) promotes tumor growth via Akt-phosphorylation and independently of Wnt-axis in Barrett&#039;s associated esophageal adenocarcinoma Am J Cancer Res, 2019;9(2):330-346. 2019 [PMID: 30906632] (Bioassay, Human) Bioassay Human
S Pérez-Garc, M Carrión, R Villanueva, T Hermida-Gó, M Fernández-, M Mellado, FJ Blanco, Y Juarranz, RP Gomariz Wnt and RUNX2 mediate cartilage breakdown by osteoarthritis synovial fibroblast-derived ADAMTS-7 and -12 J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30903650] (Bioassay, Human) Bioassay Human
F Tsurumi, S Baba, D Yoshinaga, K Umeda, T Hirata, J Takita, T Heike The intracellular Ca2+ concentration is elevated in cardiomyocytes differentiated from hiPSCs derived from a Duchenne muscular dystrophy patient PLoS ONE, 2019;14(3):e0213768. 2019 [PMID: 30875388] (Bioassay, Human) Bioassay Human
M Pontremoli, M Brioschi, R Baetta, S Ghilardi, C Banfi Identification of DKK-1 as a novel mediator of statin effects in human endothelial cells Sci Rep, 2018;8(1):16671. 2018 [PMID: 30420710] (Bioassay, Human) Bioassay Human
IF LaRocque-d, JDB Rocha, MP Nunes, PAV Oliveira, DO Nascimento, L Freire-de-, CM Takiya, A Morrot, D Decote-Ric, JO Previato, GA DosReis, L Mendonça-P, CG Freire-de- Involvement of the capsular GalXM-induced IL-17 cytokine in the control of Cryptococcus neoformans infection Sci Rep, 2018;8(1):16378. 2018 [PMID: 30401972] (Bioassay, Human) Bioassay Human
H Takeuchi, S Inagaki, W Morozumi, Y Nakano, Y Inoue, Y Kuse, T Mizoguchi, S Nakamura, M Funato, H Kaneko, H Hara, M Shimazawa VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush Sci Rep, 2018;8(1):16443. 2018 [PMID: 30401804] (Bioassay, Human) Bioassay Human
Show All 62 Publications.

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The role of Wnts in neuroinflammation
By Michalina Hanzel, PhDThe multifaceted roles of the Wnt family of glycoproteins have been extensively characterized throughout embryonic development and adult homeostasis. The highly conserved, cell- and tissue- s...  Read full blog post.

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Bioinformatics

Gene Symbol DKK1
Uniprot